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Literature summary for 1.11.1.6 extracted from

  • Chagas, R.F.; Bailao, A.M.; Fernandes, K.F.; Winters, M.S.; Pereira, M.; Soares, C.M.
    Purification of Paracoccidioides brasiliensis catalase P: subsequent kinetic and stability studies (2010), J. Biochem., 147, 345-351.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
NADPH addition of NADPH stimulates the activity of CatP in a dose-dependent manner most likely by preventing the inactivation from oxidative damage through its substrate H2O2 Paracoccidioides brasiliensis

Inhibitors

Inhibitors Comment Organism Structure
3-Amino-1,2,4-triazole specific irreversible inhibitor, complete inhibition at 20 mM Paracoccidioides brasiliensis
azide irreversible inhibitor, effective at concentrations less than1 mM Paracoccidioides brasiliensis
hydroxylamine
-
Paracoccidioides brasiliensis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
61000
-
4 * 61000, SDS-PAGE Paracoccidioides brasiliensis
244000
-
gel filtration Paracoccidioides brasiliensis

Organic Solvent Stability

Organic Solvent Comment Organism
guanidine-HCl CatP is highly resistant to denaturation by guanidine-HCl. The enzyme conserves activity after 48 h exposure to 1 M guanidine-HCl. Guanidine-HCl at 1.5 M for 48 h decreases the catalase P activity to about 60% Paracoccidioides brasiliensis

Organism

Organism UniProt Comment Textmining
Paracoccidioides brasiliensis Q8X220
-
-
Paracoccidioides brasiliensis ATCC MYA-826 Q8X220
-
-

Purification (Commentary)

Purification (Comment) Organism
acetone precipitation and Sephadex G-150 gel filtration Paracoccidioides brasiliensis

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
366.6
-
crude extract, in 50 mM sodium phosphate buffer, pH 7.8, at 37°C Paracoccidioides brasiliensis
1134
-
after 3.1fold purification, in 50 mM sodium phosphate buffer, pH 7.8, at 37°C Paracoccidioides brasiliensis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
H2O2
-
Paracoccidioides brasiliensis O2 + H2O
-
?
H2O2
-
Paracoccidioides brasiliensis ATCC MYA-826 O2 + H2O
-
?

Subunits

Subunits Comment Organism
homotetramer 4 * 61000, SDS-PAGE Paracoccidioides brasiliensis

Synonyms

Synonyms Comment Organism
catalase P
-
Paracoccidioides brasiliensis
CatP
-
Paracoccidioides brasiliensis

pH Range

pH Minimum pH Maximum Comment Organism
4 12 the purified CatP is stable and yields similar activity from pH 4.0 to 12.0 Paracoccidioides brasiliensis

pH Stability

pH Stability pH Stability Maximum Comment Organism
3.8 7.8 approximately 20% activity is observed at pH 3.8 followed by a steady rise of activity as the pH is increased to 7.8 (100% activity) Paracoccidioides brasiliensis

pI Value

Organism Comment pI Value Maximum pI Value
Paracoccidioides brasiliensis isoelectric focusing
-
6.2

IC50 Value

IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
0.0015
-
in 50 mM sodium phosphate buffer, pH 7.8, at 37°C Paracoccidioides brasiliensis azide
0.002
-
in 50 mM sodium phosphate buffer, pH 7.8, at 37°C Paracoccidioides brasiliensis hydroxylamine

Expression

Organism Comment Expression
Paracoccidioides brasiliensis CatP presents high levels of expression in the yeast parasitic phase up