Activating Compound | Comment | Organism | Structure |
---|---|---|---|
NADPH | addition of NADPH stimulates the activity of CatP in a dose-dependent manner most likely by preventing the inactivation from oxidative damage through its substrate H2O2 | Paracoccidioides brasiliensis |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
3-Amino-1,2,4-triazole | specific irreversible inhibitor, complete inhibition at 20 mM | Paracoccidioides brasiliensis | |
azide | irreversible inhibitor, effective at concentrations less than1 mM | Paracoccidioides brasiliensis | |
hydroxylamine | - |
Paracoccidioides brasiliensis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
61000 | - |
4 * 61000, SDS-PAGE | Paracoccidioides brasiliensis |
244000 | - |
gel filtration | Paracoccidioides brasiliensis |
Organic Solvent | Comment | Organism |
---|---|---|
guanidine-HCl | CatP is highly resistant to denaturation by guanidine-HCl. The enzyme conserves activity after 48 h exposure to 1 M guanidine-HCl. Guanidine-HCl at 1.5 M for 48 h decreases the catalase P activity to about 60% | Paracoccidioides brasiliensis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Paracoccidioides brasiliensis | Q8X220 | - |
- |
Paracoccidioides brasiliensis ATCC MYA-826 | Q8X220 | - |
- |
Purification (Comment) | Organism |
---|---|
acetone precipitation and Sephadex G-150 gel filtration | Paracoccidioides brasiliensis |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
366.6 | - |
crude extract, in 50 mM sodium phosphate buffer, pH 7.8, at 37°C | Paracoccidioides brasiliensis |
1134 | - |
after 3.1fold purification, in 50 mM sodium phosphate buffer, pH 7.8, at 37°C | Paracoccidioides brasiliensis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
H2O2 | - |
Paracoccidioides brasiliensis | O2 + H2O | - |
? | |
H2O2 | - |
Paracoccidioides brasiliensis ATCC MYA-826 | O2 + H2O | - |
? |
Subunits | Comment | Organism |
---|---|---|
homotetramer | 4 * 61000, SDS-PAGE | Paracoccidioides brasiliensis |
Synonyms | Comment | Organism |
---|---|---|
catalase P | - |
Paracoccidioides brasiliensis |
CatP | - |
Paracoccidioides brasiliensis |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
4 | 12 | the purified CatP is stable and yields similar activity from pH 4.0 to 12.0 | Paracoccidioides brasiliensis |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
3.8 | 7.8 | approximately 20% activity is observed at pH 3.8 followed by a steady rise of activity as the pH is increased to 7.8 (100% activity) | Paracoccidioides brasiliensis |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Paracoccidioides brasiliensis | isoelectric focusing | - |
6.2 |
IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|
0.0015 | - |
in 50 mM sodium phosphate buffer, pH 7.8, at 37°C | Paracoccidioides brasiliensis | azide | |
0.002 | - |
in 50 mM sodium phosphate buffer, pH 7.8, at 37°C | Paracoccidioides brasiliensis | hydroxylamine |
Organism | Comment | Expression |
---|---|---|
Paracoccidioides brasiliensis | CatP presents high levels of expression in the yeast parasitic phase | up |