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Literature summary for 1.11.1.6 extracted from

  • Calandrelli, V.; Gambacorta, A.; Romano, I.; Carratore, V.; Lama, L.
    A novel thermo-alkali stable catalase-peroxidase from Oceanobacillus oncorhynchi subsp. incaldaniensis: Purification and characterization (2008), World J. Microbiol. Biotechnol., 24, 2269-2275.
No PubMed abstract available

Inhibitors

Inhibitors Comment Organism Structure
3-Amino-1,2,4-triazole 3-amino-1,2,4-triazole at concentrations of 10 mM has no inhibition on the activity Oceanobacillus oncorhynchi
Ba2+ partial inhibition at 2 mM Oceanobacillus oncorhynchi
Co2+ partial inhibition at 2 mM Oceanobacillus oncorhynchi
EDTA 10 mM EDTA reduces activity by 27% Oceanobacillus oncorhynchi
H2O2 H2O2 causes 17% inhibition at 80 mM Oceanobacillus oncorhynchi
histidine 10 mM histidine reduces activity by 19% Oceanobacillus oncorhynchi
Hydroxylamine hydrochloride strong inhibition Oceanobacillus oncorhynchi
Sodium azide strong inhibition Oceanobacillus oncorhynchi

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
24
-
H2O2 in 20 mM Tris-HCl buffer (pH 9.0), at 30°C Oceanobacillus oncorhynchi

Metals/Ions

Metals/Ions Comment Organism Structure
Fe2+ contains 1 atom Fe2+ per homodimer Oceanobacillus oncorhynchi
K+ stimulatory effect at 2 mM Oceanobacillus oncorhynchi
Mg2+ stimulatory effect at 2 mM Oceanobacillus oncorhynchi
Mn2+ stimulatory effect at 2 mM Oceanobacillus oncorhynchi
Na+ stimulatory effect at 2 mM Oceanobacillus oncorhynchi
NaCl there is a stimulation of catalase by addition of NaCl 10 mM (176%) Oceanobacillus oncorhynchi

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
72000
-
4 * 72000, SDS-PAGE Oceanobacillus oncorhynchi
280000
-
gel filtration Oceanobacillus oncorhynchi

Organism

Organism UniProt Comment Textmining
Oceanobacillus oncorhynchi
-
subspecies Oceanobacillus oncorhynchi incaldaniensis, strain 20AG
-

Purification (Commentary)

Purification (Comment) Organism
Q-Sepharose column chromatography, Superdex 200 gel filtration, and Mono Q column chromatography Oceanobacillus oncorhynchi

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
316
-
crude extract, at 30°C Oceanobacillus oncorhynchi
35000
-
after 110.7fold purification, at 30°C Oceanobacillus oncorhynchi

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
H2O2
-
Oceanobacillus oncorhynchi O2 + H2O
-
?

Subunits

Subunits Comment Organism
homotetramer 4 * 72000, SDS-PAGE Oceanobacillus oncorhynchi

Synonyms

Synonyms Comment Organism
catalase-peroxidase
-
Oceanobacillus oncorhynchi
H2O2:H2O2 oxidoreductase
-
Oceanobacillus oncorhynchi

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
40
-
-
Oceanobacillus oncorhynchi

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
30 60
-
Oceanobacillus oncorhynchi

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
40 60 the enzyme is stable for 24 h at 40°C with a half-life of 4 h 60°C, at 50°C the half life is about 18 h Oceanobacillus oncorhynchi

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
9
-
-
Oceanobacillus oncorhynchi

pH Range

pH Minimum pH Maximum Comment Organism
5 10
-
Oceanobacillus oncorhynchi

pH Stability

pH Stability pH Stability Maximum Comment Organism
5 10 the enzyme is stable in the pH range of 5.0 to 10.0 after 1 h of treatment at 40°C Oceanobacillus oncorhynchi

Cofactor

Cofactor Comment Organism Structure
heme contains 1 heme per homodimer Oceanobacillus oncorhynchi