Inhibitors | Comment | Organism | Structure |
---|---|---|---|
3-Amino-1,2,4-triazole | 3-amino-1,2,4-triazole at concentrations of 10 mM has no inhibition on the activity | Oceanobacillus oncorhynchi | |
Ba2+ | partial inhibition at 2 mM | Oceanobacillus oncorhynchi | |
Co2+ | partial inhibition at 2 mM | Oceanobacillus oncorhynchi | |
EDTA | 10 mM EDTA reduces activity by 27% | Oceanobacillus oncorhynchi | |
H2O2 | H2O2 causes 17% inhibition at 80 mM | Oceanobacillus oncorhynchi | |
histidine | 10 mM histidine reduces activity by 19% | Oceanobacillus oncorhynchi | |
Hydroxylamine hydrochloride | strong inhibition | Oceanobacillus oncorhynchi | |
Sodium azide | strong inhibition | Oceanobacillus oncorhynchi |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
24 | - |
H2O2 | in 20 mM Tris-HCl buffer (pH 9.0), at 30°C | Oceanobacillus oncorhynchi |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Fe2+ | contains 1 atom Fe2+ per homodimer | Oceanobacillus oncorhynchi | |
K+ | stimulatory effect at 2 mM | Oceanobacillus oncorhynchi | |
Mg2+ | stimulatory effect at 2 mM | Oceanobacillus oncorhynchi | |
Mn2+ | stimulatory effect at 2 mM | Oceanobacillus oncorhynchi | |
Na+ | stimulatory effect at 2 mM | Oceanobacillus oncorhynchi | |
NaCl | there is a stimulation of catalase by addition of NaCl 10 mM (176%) | Oceanobacillus oncorhynchi |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
72000 | - |
4 * 72000, SDS-PAGE | Oceanobacillus oncorhynchi |
280000 | - |
gel filtration | Oceanobacillus oncorhynchi |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Oceanobacillus oncorhynchi | - |
subspecies Oceanobacillus oncorhynchi incaldaniensis, strain 20AG | - |
Purification (Comment) | Organism |
---|---|
Q-Sepharose column chromatography, Superdex 200 gel filtration, and Mono Q column chromatography | Oceanobacillus oncorhynchi |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
316 | - |
crude extract, at 30°C | Oceanobacillus oncorhynchi |
35000 | - |
after 110.7fold purification, at 30°C | Oceanobacillus oncorhynchi |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
H2O2 | - |
Oceanobacillus oncorhynchi | O2 + H2O | - |
? |
Subunits | Comment | Organism |
---|---|---|
homotetramer | 4 * 72000, SDS-PAGE | Oceanobacillus oncorhynchi |
Synonyms | Comment | Organism |
---|---|---|
catalase-peroxidase | - |
Oceanobacillus oncorhynchi |
H2O2:H2O2 oxidoreductase | - |
Oceanobacillus oncorhynchi |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
- |
Oceanobacillus oncorhynchi |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | 60 | - |
Oceanobacillus oncorhynchi |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | 60 | the enzyme is stable for 24 h at 40°C with a half-life of 4 h 60°C, at 50°C the half life is about 18 h | Oceanobacillus oncorhynchi |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
9 | - |
- |
Oceanobacillus oncorhynchi |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5 | 10 | - |
Oceanobacillus oncorhynchi |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
5 | 10 | the enzyme is stable in the pH range of 5.0 to 10.0 after 1 h of treatment at 40°C | Oceanobacillus oncorhynchi |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
heme | contains 1 heme per homodimer | Oceanobacillus oncorhynchi |