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Literature summary for 1.11.1.6 extracted from

  • Jeoung, J.H.; Pippig, D.A.; Martins, B.M.; Wagener, N.; Dobbek, H.
    HTHP: a novel class of hexameric, tyrosine-coordinated heme proteins (2007), J. Mol. Biol., 368, 1122-1131.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli Rosetta (DE3) Ruegeria pomeroyi DSS-3

Crystallization (Commentary)

Crystallization (Comment) Organism
-
Ruegeria pomeroyi DSS-3

Inhibitors

Inhibitors Comment Organism Structure
3-Amino-1,2,4-triazole 500 microM reduces the reactivity of catalase to 50% Ruegeria pomeroyi DSS-3

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
17.2
-
H2O2
-
Ruegeria pomeroyi DSS-3

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
8300
-
ring-like arrangement of six monomers, 6 * 7900 (SDS-PAGE), 6 * 8300 calculated Ruegeria pomeroyi DSS-3
54000
-
gel filtration Ruegeria pomeroyi DSS-3

Organism

Organism UniProt Comment Textmining
Ruegeria pomeroyi DSS-3 Q5LLG6
-
-

Purification (Commentary)

Purification (Comment) Organism
purified to homogeneity as holoprotein by affinity chromatography Ruegeria pomeroyi DSS-3

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
H2O2
-
Ruegeria pomeroyi DSS-3 O2 + H2O
-
?

Subunits

Subunits Comment Organism
hexamer ring-like arrangement of six monomers, 6 * 7900 (SDS-PAGE), 6 * 8300 calculated Ruegeria pomeroyi DSS-3

Synonyms

Synonyms Comment Organism
HTHP peroxidase and catalase activity Ruegeria pomeroyi DSS-3
tyrosine-coordinated heme protein
-
Ruegeria pomeroyi DSS-3

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
23
-
highest specific activity Ruegeria pomeroyi DSS-3

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
additional information
-
high thermal stability. HTHP is stable under a wide range of temperatures. Thermal unfolding is measured up to 110°C and in the presence of high concentrations of guanidinium hydrochloride. The melting point of HTHP is estimated to be around 130°C. Catalatic activity is tested after incubation for 10 min at 85°C and 90°C and is found to be reduced by less than 5% under both conditions Ruegeria pomeroyi DSS-3

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
10.5
-
highest specific activity Ruegeria pomeroyi DSS-3

pH Stability

pH Stability pH Stability Maximum Comment Organism
additional information
-
HTHP is stable under a wide range of pH values Ruegeria pomeroyi DSS-3