Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 1.11.1.6 extracted from

  • Wang, W.; Sun, M.; Liu, W.; Zhang, B.
    Purification and characterization of a psychrophilic catalase from Antarctic Bacillus (2008), Can. J. Microbiol., 54, 823-828.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
azide
-
Bacillus sp. (in: Bacteria)
beta-mercaptoethanol
-
Bacillus sp. (in: Bacteria)
hydroxylamine
-
Bacillus sp. (in: Bacteria)

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
41.5
-
H2O2 in 50 mM potassium phosphate buffer (pH 7.0), at 4°C Bacillus sp. (in: Bacteria)
43.6
-
H2O2 in 50 mM potassium phosphate buffer (pH 7.0), at 25°C Bacillus sp. (in: Bacteria)

Metals/Ions

Metals/Ions Comment Organism Structure
Fe3+ the active site of the enzyme contains Fe3+ Bacillus sp. (in: Bacteria)

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
56000
-
4 * 56000, gel filtration Bacillus sp. (in: Bacteria)
230000
-
gel filtration Bacillus sp. (in: Bacteria)

Organism

Organism UniProt Comment Textmining
Bacillus sp. (in: Bacteria)
-
-
-
Bacillus sp. (in: Bacteria) N2a
-
-
-

Purification (Commentary)

Purification (Comment) Organism
ammonium sulfate precipitation, DEAE-Sephadex gel filtration, and Sephacryl gel filtration Bacillus sp. (in: Bacteria)

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
H2O2
-
Bacillus sp. (in: Bacteria) O2 + H2O
-
?
H2O2
-
Bacillus sp. (in: Bacteria) N2a O2 + H2O
-
?
additional information BNC does not show any peroxidatic activity with 4-aminoantipyrine or pyrogallol as substrate Bacillus sp. (in: Bacteria) ?
-
?
additional information BNC does not show any peroxidatic activity with 4-aminoantipyrine or pyrogallol as substrate Bacillus sp. (in: Bacteria) N2a ?
-
?

Subunits

Subunits Comment Organism
homotetramer 4 * 56000, gel filtration Bacillus sp. (in: Bacteria)

Synonyms

Synonyms Comment Organism
BNC
-
Bacillus sp. (in: Bacteria)

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
-
Bacillus sp. (in: Bacteria)

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
50 60 65% residual activity after 15 min at 50°C, 54% residual activity after 30 min at 50°C, 43% residual activity after 40 min at 50°C, 12% residual activity after 15 min of preincubation at 60°C, preincubation at 65°C for 10 min results in complete loss of activity Bacillus sp. (in: Bacteria)

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
149000
-
H2O2 in 50 mM potassium phosphate buffer (pH 7.0), at 4°C Bacillus sp. (in: Bacteria)
175000
-
H2O2 in 50 mM potassium phosphate buffer (pH 7.0), at 25°C Bacillus sp. (in: Bacteria)

pH Range

pH Minimum pH Maximum Comment Organism
6 11
-
Bacillus sp. (in: Bacteria)

Cofactor

Cofactor Comment Organism Structure
heme the active site of the enzyme contains heme Bacillus sp. (in: Bacteria)

pI Value

Organism Comment pI Value Maximum pI Value
Bacillus sp. (in: Bacteria) isoelectric focusing
-
4.2

IC50 Value

IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
0.0003
-
-
Bacillus sp. (in: Bacteria) hydroxylamine
0.0017
-
-
Bacillus sp. (in: Bacteria) azide
2
-
-
Bacillus sp. (in: Bacteria) beta-mercaptoethanol