Application | Comment | Organism |
---|---|---|
medicine | systemic reduction in catalase activity by dsRNA-mediated knock-down significantly reduces the reproductive output of mosquito females. Mutation S2W leads to strain G3 which shows a lower specific activity and higher Km value than the wild-type Ser-isoform. Mutation S2W destabilizes the functional tetrameric form of the enzyme. Ser/Ser females have a significantly higher fecundity than Trp/Trp females | Anopheles gambiae |
Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Anopheles gambiae |
Protein Variants | Comment | Organism |
---|---|---|
additional information | systemic reduction in catalase activity by dsRNA-mediated knock-down significantly reduces the reproductive output of mosquito females | Anopheles gambiae |
S2W | natural polymorphism. Strain S carries the Ser isoform, strain G3 carries the Trp-isoform which shows a lower specific activity and higher Km value than the Ser-isoform. Mutation S2W destabilizes the functional tetrameric form of the enzyme. Ser/Ser females have a significantly higher fecundity than Trp/Trp females | Anopheles gambiae |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
42 | - |
H2O2 | wild-type | Anopheles gambiae | |
65.8 | - |
H2O2 | mutant S2W | Anopheles gambiae |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Anopheles gambiae | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
H2O2 | - |
Anopheles gambiae | O2 + H2O | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
both wild-type and mutant S2W | Anopheles gambiae |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
both wild-type and mutant S2W | Anopheles gambiae |