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Literature summary for 1.11.1.6 extracted from

  • DeJong, R.J.; Miller, L.M.; Molina-Cruz, A.; Gupta, L.; Kumar, S.; Barillas-Mury, C.
    Reactive oxygen species detoxification by catalase is a major determinant of fecundity in the mosquito Anopheles gambiae (2007), Proc. Natl. Acad. Sci. USA, 104, 2121-2126.
    View publication on PubMedView publication on EuropePMC

Application

Application Comment Organism
medicine systemic reduction in catalase activity by dsRNA-mediated knock-down significantly reduces the reproductive output of mosquito females. Mutation S2W leads to strain G3 which shows a lower specific activity and higher Km value than the wild-type Ser-isoform. Mutation S2W destabilizes the functional tetrameric form of the enzyme. Ser/Ser females have a significantly higher fecundity than Trp/Trp females Anopheles gambiae

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Anopheles gambiae

Protein Variants

Protein Variants Comment Organism
additional information systemic reduction in catalase activity by dsRNA-mediated knock-down significantly reduces the reproductive output of mosquito females Anopheles gambiae
S2W natural polymorphism. Strain S carries the Ser isoform, strain G3 carries the Trp-isoform which shows a lower specific activity and higher Km value than the Ser-isoform. Mutation S2W destabilizes the functional tetrameric form of the enzyme. Ser/Ser females have a significantly higher fecundity than Trp/Trp females Anopheles gambiae

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
42
-
H2O2 wild-type Anopheles gambiae
65.8
-
H2O2 mutant S2W Anopheles gambiae

Organism

Organism UniProt Comment Textmining
Anopheles gambiae
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
H2O2
-
Anopheles gambiae O2 + H2O
-
?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
40
-
both wild-type and mutant S2W Anopheles gambiae

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
both wild-type and mutant S2W Anopheles gambiae