Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli as fusion protein with maltose binding protein | Haliotis discus discus |
Crystallization (Comment) | Organism |
---|---|
homology modeling using crystal structures of human and bovine catalases | Haliotis discus discus |
Protein Variants | Comment | Organism |
---|---|---|
additional information | both native protein and fusion protein with maltose binding protein show the same specific activity | Haliotis discus discus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
56000 | - |
x * 56000, calculated, x * 98.5, SDS-PAGE of fusion protein with maltose binding protein | Haliotis discus discus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Haliotis discus discus | A5XB38 | - |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
adductor | constitutive expression for the first 3 h post injection of H2O2 | Haliotis discus discus | - |
digestive gland | constitutive expression for the first 3 h post injection of H2O2 | Haliotis discus discus | - |
gill | constitutive expression for the first 3 h post injection of H2O2 | Haliotis discus discus | - |
gonad | constitutive expression for the first 3 h post injection of H2O2 | Haliotis discus discus | - |
hemocyte | constitutive expression for the first 3 h post injection of H2O2 | Haliotis discus discus | - |
mantle | constitutive expression for the first 3 h post injection of H2O2 | Haliotis discus discus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
30000 | - |
37°C, pH 5.0 | Haliotis discus discus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
H2O2 | - |
Haliotis discus discus | O2 + H2O | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 56000, calculated, x * 98.5, SDS-PAGE of fusion protein with maltose binding protein | Haliotis discus discus |
Synonyms | Comment | Organism |
---|---|---|
Ab-catalase | - |
Haliotis discus discus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | 37 | - |
Haliotis discus discus |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | 70 | slight decrease in activity compared to temperature optimum | Haliotis discus discus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
1 h, 60% residual activity | Haliotis discus discus |
50 | - |
rapid inactivation above | Haliotis discus discus |
70 | - |
20 min, complete loss of activity | Haliotis discus discus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
10.5 | - |
- |
Haliotis discus discus |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
4.5 | 10.5 | above 90% of maximum activity | Haliotis discus discus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
heme | deduced from sequence | Haliotis discus discus | |
NADPH | deduced from sequence | Haliotis discus discus |