Application | Comment | Organism |
---|---|---|
synthesis | production of recombinant Bacillus subtilis catalase and purification from culture fluid. Purified enzyme has a specific activity of 34600 U/mg and is more resistant to acidic conditions than bovine liver catalase | Bacillus subtilis |
Cloned (Comment) | Organism |
---|---|
expression in Bacillus subtilis | Bacillus subtilis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
52.5 | - |
H2O2 | - |
Bacillus subtilis |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
extracellular | - |
Bacillus subtilis | - |
- |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
60000 | - |
4 * 60000, SDS-PAGE | Bacillus subtilis |
240000 | - |
PAGE | Bacillus subtilis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus subtilis | Q9AQQ9 | KatA | - |
Bacillus subtilis KatA | Q9AQQ9 | KatA | - |
Purification (Comment) | Organism |
---|---|
recombinant enzyme | Bacillus subtilis |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
34600 | - |
- |
Bacillus subtilis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
H2O2 | - |
Bacillus subtilis | O2 + H2O | - |
? | |
H2O2 | - |
Bacillus subtilis KatA | O2 + H2O | - |
? |
Subunits | Comment | Organism |
---|---|---|
tetramer | 4 * 60000, SDS-PAGE | Bacillus subtilis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
- |
Bacillus subtilis |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
15 h, incubation below 40°C, retains most of its activity. Activity is quickly lost above 40°C | Bacillus subtilis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6 | - |
- |
Bacillus subtilis |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5 | 8 | - |
Bacillus subtilis |