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Literature summary for 1.11.1.6 extracted from

  • Sutay Kocabas, D.; Bakir, U.; Phillips, S.E.; McPherson, M.J.; Ogel, Z.B.
    Purification, characterization, and identification of a novel bifunctional catalase-phenol oxidase from Scytalidium thermophilum (2008), Appl. Microbiol. Biotechnol., 79, 407-415.
    View publication on PubMed

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular
-
Mycothermus thermophilus
-
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
80000
-
4 * 80000, SDS-PAGE Mycothermus thermophilus
320000
-
PAGE Mycothermus thermophilus

Organism

Organism UniProt Comment Textmining
Mycothermus thermophilus
-
bifunctional catalase-phenol oxidase
-

Purification (Commentary)

Purification (Comment) Organism
-
Mycothermus thermophilus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
444.4
-
60°C, pH 7.0 Mycothermus thermophilus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
H2O2
-
Mycothermus thermophilus O2 + H2O
-
?

Subunits

Subunits Comment Organism
tetramer 4 * 80000, SDS-PAGE Mycothermus thermophilus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
60
-
more than 70% of catalase maximum activity within Mycothermus thermophilus

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
30 75
-
Mycothermus thermophilus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
both catalase and phenol oxidase activities Mycothermus thermophilus

pH Range

pH Minimum pH Maximum Comment Organism
5 9 more than 60% of catalase maximum activity within Mycothermus thermophilus
6 8 more than 80% of catalase maximum activity within Mycothermus thermophilus

pH Stability

pH Stability pH Stability Maximum Comment Organism
7
-
24 h, more than 90% of initial activity Mycothermus thermophilus

Cofactor

Cofactor Comment Organism Structure
heme
-
Mycothermus thermophilus

pI Value

Organism Comment pI Value Maximum pI Value
Mycothermus thermophilus isoelectric focusing
-
5