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Literature summary for 1.11.1.6 extracted from

  • Brown, S.M.; Howell, M.L.; Vasil, M.L.; et al.
    Cloning and characterization of the katB gene of Pseudomonas aeruginosa encoding a hydrogen peroxide-inducible catalase: purification of KatB, cellular localization, and demonstration that it is essential for optimal resistance to hydrogen peroxide (1995), J. Bacteriol., 177, 6536-6544.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
katB, expression in Escherichia coli Pseudomonas aeruginosa

Localization

Localization Comment Organism GeneOntology No. Textmining
cytoplasm
-
Pseudomonas aeruginosa 5737
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
57000
-
4 * 57000, SDS-PAGE Pseudomonas aeruginosa
228000
-
gel filtration Pseudomonas aeruginosa

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
H2O2 Pseudomonas aeruginosa essential for optimal resistance to H2O2. KatB was not produced during the normal growth cycle. Catalase activity is greater in nonmucoid than in mucoid, alginate-producing organisms. When exposed to hydrogen peroxide and, to a greater extent, paraquat, total catalase activity was elevated 7fold to 16fold, respectively O2 + H2O
-
?

Organism

Organism UniProt Comment Textmining
Pseudomonas aeruginosa
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Pseudomonas aeruginosa

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
H2O2 essential for optimal resistance to H2O2. KatB was not produced during the normal growth cycle. Catalase activity is greater in nonmucoid than in mucoid, alginate-producing organisms. When exposed to hydrogen peroxide and, to a greater extent, paraquat, total catalase activity was elevated 7fold to 16fold, respectively Pseudomonas aeruginosa O2 + H2O
-
?

Subunits

Subunits Comment Organism
tetramer 4 * 57000, SDS-PAGE Pseudomonas aeruginosa

Synonyms

Synonyms Comment Organism
KatB
-
Pseudomonas aeruginosa

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
58
-
1 h, 83% reduction in activity Pseudomonas aeruginosa
60
-
1 h, complete loss of activity Pseudomonas aeruginosa

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7 9.5
-
Pseudomonas aeruginosa

pH Stability

pH Stability pH Stability Maximum Comment Organism
3
-
inactivation Pseudomonas aeruginosa