Activating Compound | Comment | Organism | Structure |
---|---|---|---|
hemin | 0.002-0.01 mM required for half-maximal to maximal enzyme production | Enterococcus faecalis |
Cloned (Comment) | Organism |
---|---|
expression as His-tagged enzyme in constructed enzyme-deficient Bacillus subtilis strain BLF03, overexpression of wild-type and His-tagged enzyme in Enterococcus faecalis strain V583 utilizing an Escherichia coli-Enterococcus faecalis-shuttle plasmid | Enterococcus faecalis |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytoplasm | - |
Enterococcus faecalis | 5737 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Fe2+ | heme-containing enzyme | Enterococcus faecalis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
54000 | - |
x * 54000, recombinant His-tagged enzyme, SDS-PAGE | Enterococcus faecalis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
H2O2 | Enterococcus faecalis | detoxification enzyme, enzyme protects the cell against high concentrations of peroxide | O2 + H2O | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Enterococcus faecalis | - |
gene katA | - |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged and wild-type enzymes from Bacillus subtilis strain BLF03, 195fold, and Enterococcus faecalis strain V583 | Enterococcus faecalis |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
culture condition:heme-grown cell | heme in the growth medium is absolutely required for enzyme expression | Enterococcus faecalis | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Enterococcus faecalis |
82 | - |
purified recombinant His-tagged enzyme expressed in Bacillus subtilis | Enterococcus faecalis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
H2O2 | - |
Enterococcus faecalis | O2 + H2O | - |
? | |
H2O2 | detoxification enzyme, enzyme protects the cell against high concentrations of peroxide | Enterococcus faecalis | O2 + H2O | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 54000, recombinant His-tagged enzyme, SDS-PAGE | Enterococcus faecalis |
Synonyms | Comment | Organism |
---|---|---|
KatA | - |
Enterococcus faecalis |
More | enzyme belongs to the family of monofunctional catalases | Enterococcus faecalis |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
heme | dependent on, contains 1 protoheme IX group per KatA 54 kDa polypeptide | Enterococcus faecalis |