Activating Compound | Comment | Organism | Structure |
---|---|---|---|
additional information | no effect by 0.01 mM hydrogen peroxide or 100 nM paraquat, 3.4fold increase in intracellular activity by elimination of thiosulfate from the aerobic growth medium | Pyrobaculum calidifontis |
Cloned (Comment) | Organism |
---|---|
gene katPc, DNA and amino acid sequence determination and analysis | Pyrobaculum calidifontis |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
additional information | no effect by 0.01 mM hydrogen peroxide or 100 nM paraquat | Pyrobaculum calidifontis | |
Sodium azide | only 10% inhibition at 1 mM | Pyrobaculum calidifontis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
170 | - |
H2O2 | pH 7.0, 25°C | Pyrobaculum calidifontis |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mn2+ | activates at 0.001 mM in vivo, 1.32 atoms bound per subunit | Pyrobaculum calidifontis | |
additional information | enzyme contains no heme and no Fe2+ | Pyrobaculum calidifontis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
33450 | - |
4 * 33450, amino acid sequence calculation, 4 * 38000, SDS-PAGE | Pyrobaculum calidifontis |
38000 | - |
4 * 33450, amino acid sequence calculation, 4 * 38000, SDS-PAGE | Pyrobaculum calidifontis |
154000 | - |
gel filtration | Pyrobaculum calidifontis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
H2O2 | Pyrobaculum calidifontis | detoxification enzyme | O2 + H2O | - |
? | |
H2O2 | Pyrobaculum calidifontis VA1 | detoxification enzyme | O2 + H2O | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pyrobaculum calidifontis | Q6LA34 | hyperthermophilic archaeon, facultatively anaerobic, gene katPc | - |
Pyrobaculum calidifontis VA1 | Q6LA34 | hyperthermophilic archaeon, facultatively anaerobic, gene katPc | - |
Purification (Comment) | Organism |
---|---|
91fold from crude cell extract | Pyrobaculum calidifontis |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
additional information | high enzyme activity when the strain VA1 is grown under aerobic conditions, and decreased activity under anaerobic growth | Pyrobaculum calidifontis | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
23500 | - |
91fold purified enzyme, at 70°C | Pyrobaculum calidifontis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
H2O2 | detoxification enzyme | Pyrobaculum calidifontis | O2 + H2O | - |
? | |
H2O2 | with pyrogallol as electron donor | Pyrobaculum calidifontis | O2 + H2O | - |
? | |
H2O2 | detoxification enzyme | Pyrobaculum calidifontis VA1 | O2 + H2O | - |
? | |
H2O2 | with pyrogallol as electron donor | Pyrobaculum calidifontis VA1 | O2 + H2O | - |
? |
Subunits | Comment | Organism |
---|---|---|
tetramer | 4 * 33450, amino acid sequence calculation, 4 * 38000, SDS-PAGE | Pyrobaculum calidifontis |
Synonyms | Comment | Organism |
---|---|---|
manganese catalase | - |
Pyrobaculum calidifontis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
90 | - |
at pH 7.0 | Pyrobaculum calidifontis |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | 100 | range of significant activity | Pyrobaculum calidifontis |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
90 | - |
half-life: 432 min | Pyrobaculum calidifontis |
95 | - |
half-life: 114 min | Pyrobaculum calidifontis |
100 | - |
half-life: 50 min | Pyrobaculum calidifontis |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
29000 | - |
H2O2 | pH 7.0, 25°C, per subunit | Pyrobaculum calidifontis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
9.5 | - |
in glycine-NaOH buffer, at 70°C | Pyrobaculum calidifontis |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
4 | 10 | - |
Pyrobaculum calidifontis |