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Literature summary for 1.11.1.6 extracted from

  • Amo, T.; Atomi, H.; Imanaka, T.
    Unique presence of a manganese catalase in a hyperthermophilic archaeon, Pyrobaculum calidifontis VA1 (2002), J. Bacteriol., 184, 3305-3312.
    View publication on PubMedView publication on EuropePMC

Activating Compound

Activating Compound Comment Organism Structure
additional information no effect by 0.01 mM hydrogen peroxide or 100 nM paraquat, 3.4fold increase in intracellular activity by elimination of thiosulfate from the aerobic growth medium Pyrobaculum calidifontis

Cloned(Commentary)

Cloned (Comment) Organism
gene katPc, DNA and amino acid sequence determination and analysis Pyrobaculum calidifontis

Inhibitors

Inhibitors Comment Organism Structure
additional information no effect by 0.01 mM hydrogen peroxide or 100 nM paraquat Pyrobaculum calidifontis
Sodium azide only 10% inhibition at 1 mM Pyrobaculum calidifontis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
170
-
H2O2 pH 7.0, 25°C Pyrobaculum calidifontis

Metals/Ions

Metals/Ions Comment Organism Structure
Mn2+ activates at 0.001 mM in vivo, 1.32 atoms bound per subunit Pyrobaculum calidifontis
additional information enzyme contains no heme and no Fe2+ Pyrobaculum calidifontis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
33450
-
4 * 33450, amino acid sequence calculation, 4 * 38000, SDS-PAGE Pyrobaculum calidifontis
38000
-
4 * 33450, amino acid sequence calculation, 4 * 38000, SDS-PAGE Pyrobaculum calidifontis
154000
-
gel filtration Pyrobaculum calidifontis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
H2O2 Pyrobaculum calidifontis detoxification enzyme O2 + H2O
-
?
H2O2 Pyrobaculum calidifontis VA1 detoxification enzyme O2 + H2O
-
?

Organism

Organism UniProt Comment Textmining
Pyrobaculum calidifontis Q6LA34 hyperthermophilic archaeon, facultatively anaerobic, gene katPc
-
Pyrobaculum calidifontis VA1 Q6LA34 hyperthermophilic archaeon, facultatively anaerobic, gene katPc
-

Purification (Commentary)

Purification (Comment) Organism
91fold from crude cell extract Pyrobaculum calidifontis

Source Tissue

Source Tissue Comment Organism Textmining
additional information high enzyme activity when the strain VA1 is grown under aerobic conditions, and decreased activity under anaerobic growth Pyrobaculum calidifontis
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
23500
-
91fold purified enzyme, at 70°C Pyrobaculum calidifontis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
H2O2 detoxification enzyme Pyrobaculum calidifontis O2 + H2O
-
?
H2O2 with pyrogallol as electron donor Pyrobaculum calidifontis O2 + H2O
-
?
H2O2 detoxification enzyme Pyrobaculum calidifontis VA1 O2 + H2O
-
?
H2O2 with pyrogallol as electron donor Pyrobaculum calidifontis VA1 O2 + H2O
-
?

Subunits

Subunits Comment Organism
tetramer 4 * 33450, amino acid sequence calculation, 4 * 38000, SDS-PAGE Pyrobaculum calidifontis

Synonyms

Synonyms Comment Organism
manganese catalase
-
Pyrobaculum calidifontis

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
90
-
at pH 7.0 Pyrobaculum calidifontis

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
30 100 range of significant activity Pyrobaculum calidifontis

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
90
-
half-life: 432 min Pyrobaculum calidifontis
95
-
half-life: 114 min Pyrobaculum calidifontis
100
-
half-life: 50 min Pyrobaculum calidifontis

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
29000
-
H2O2 pH 7.0, 25°C, per subunit Pyrobaculum calidifontis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
9.5
-
in glycine-NaOH buffer, at 70°C Pyrobaculum calidifontis

pH Range

pH Minimum pH Maximum Comment Organism
4 10
-
Pyrobaculum calidifontis