Crystallization (Comment) | Organism |
---|---|
ammonium sulfate precipitation | Kloeckera sp. |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
H2O2 | inhibition above 50 mM | Kloeckera sp. |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
25 | - |
H2O2 | catalase activity | Kloeckera sp. | |
83 | - |
methanol | peroxidase activity | Kloeckera sp. |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
peroxisome | - |
Kloeckera sp. | 5777 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
62000 | - |
4 * 62000, SDS-PAGE | Kloeckera sp. |
240000 | - |
gel filtration | Kloeckera sp. |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
H2O2 | Kloeckera sp. | - |
O2 + H2O | - |
? | |
H2O2 | Kloeckera sp. 2201 | - |
O2 + H2O | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Kloeckera sp. | - |
- |
- |
Kloeckera sp. 2201 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
to homogeneity, chromatography steps | Kloeckera sp. |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
cell culture | methanol grown | Kloeckera sp. | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
282000 | - |
- |
Kloeckera sp. |
Storage Stability | Organism |
---|---|
4°C, more than six months | Kloeckera sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
beta-(3,4-dihydroxyphenyl)-L-alanine + H2O2 | low activity | Kloeckera sp. | ? + H2O | - |
? | |
beta-(3,4-dihydroxyphenyl)-L-alanine + H2O2 | low activity | Kloeckera sp. 2201 | ? + H2O | - |
? | |
H2O2 | - |
Kloeckera sp. | O2 + H2O | - |
? | |
H2O2 | - |
Kloeckera sp. 2201 | O2 + H2O | - |
? | |
methanol + H2O2 | only in the presence of glucose and glucose oxidase | Kloeckera sp. | formaldehyde + ? | - |
? | |
methanol + H2O2 | only in the presence of glucose and glucose oxidase | Kloeckera sp. 2201 | formaldehyde + ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
tetramer | 4 * 62000, SDS-PAGE | Kloeckera sp. |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.2 | - |
- |
Kloeckera sp. |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5 | 9 | activity within | Kloeckera sp. |