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Literature summary for 1.11.1.6 extracted from

  • Mozaffar, S.; Ueda, M.; Kitatsuji, K.; Shimizu, S.; Osumi, M.; Tanaka, A.
    Properties of catalase purified from a methanol-grown yeast, Kloeckera sp. 2201 (1986), Eur. J. Biochem., 155, 527-531.
    View publication on PubMed

Crystallization (Commentary)

Crystallization (Comment) Organism
ammonium sulfate precipitation Kloeckera sp.

Inhibitors

Inhibitors Comment Organism Structure
H2O2 inhibition above 50 mM Kloeckera sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
25
-
H2O2 catalase activity Kloeckera sp.
83
-
methanol peroxidase activity Kloeckera sp.

Localization

Localization Comment Organism GeneOntology No. Textmining
peroxisome
-
Kloeckera sp. 5777
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
62000
-
4 * 62000, SDS-PAGE Kloeckera sp.
240000
-
gel filtration Kloeckera sp.

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
H2O2 Kloeckera sp.
-
O2 + H2O
-
?
H2O2 Kloeckera sp. 2201
-
O2 + H2O
-
?

Organism

Organism UniProt Comment Textmining
Kloeckera sp.
-
-
-
Kloeckera sp. 2201
-
-
-

Purification (Commentary)

Purification (Comment) Organism
to homogeneity, chromatography steps Kloeckera sp.

Source Tissue

Source Tissue Comment Organism Textmining
cell culture methanol grown Kloeckera sp.
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
282000
-
-
Kloeckera sp.

Storage Stability

Storage Stability Organism
4°C, more than six months Kloeckera sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
beta-(3,4-dihydroxyphenyl)-L-alanine + H2O2 low activity Kloeckera sp. ? + H2O
-
?
beta-(3,4-dihydroxyphenyl)-L-alanine + H2O2 low activity Kloeckera sp. 2201 ? + H2O
-
?
H2O2
-
Kloeckera sp. O2 + H2O
-
?
H2O2
-
Kloeckera sp. 2201 O2 + H2O
-
?
methanol + H2O2 only in the presence of glucose and glucose oxidase Kloeckera sp. formaldehyde + ?
-
?
methanol + H2O2 only in the presence of glucose and glucose oxidase Kloeckera sp. 2201 formaldehyde + ?
-
?

Subunits

Subunits Comment Organism
tetramer 4 * 62000, SDS-PAGE Kloeckera sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.2
-
-
Kloeckera sp.

pH Range

pH Minimum pH Maximum Comment Organism
5 9 activity within Kloeckera sp.