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Literature summary for 1.11.1.5 extracted from

  • Schuetz, B.; Seidel, J.; Sturm, G.; Einsle, O.; Gescher, J.
    Investigation of the electron transport chain to and the catalytic activity of the diheme cytochrome c peroxidase CcpA of Shewanella oneidensis (2011), Appl. Environ. Microbiol., 77, 6172-6180.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
gene ccpA, expression of His-tagged CcpA in Escherichia coli strain AS457 Shewanella oneidensis

Crystallization (Commentary)

Crystallization (Comment) Organism
purified recombinnat His-tagged CcpA, sitting drop vapor diffusion, mixing of 0.001 ml 7.5 mg/ml dithionite-reduced protein solution with 0.001 ml of reservoir solution containing 26% w/v PEG 2000 monomethyl ether and 0.1 M bis-(2-hydroxyethyl)-amino-tris-(hydroxymethyl)methane, pH 5.0, equilibration against 0.2 ml of reserrvoir solution, 10% v/v (2R,3R)-butanediol as a cryoprotectant, X-ray diffraction structure determination and analysis Shewanella oneidensis

Protein Variants

Protein Variants Comment Organism
additional information construction of a DELTAccpA mutant Shewanella oneidensis line Shewanella oneidensis

Inhibitors

Inhibitors Comment Organism Structure
2-heptyl-4-hydroxyquinoline N-oxide
-
Shewanella oneidensis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0113
-
2,2-azinobis(3-ethylbenzthiazolinesulfonic acid) pH 7.5, temperature not specified in the publication Shewanella oneidensis

Localization

Localization Comment Organism GeneOntology No. Textmining
periplasm
-
Shewanella oneidensis
-
-

Metals/Ions

Metals/Ions Comment Organism Structure
Fe2+ diheme cytochrome c peroxidase Shewanella oneidensis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Shewanella oneidensis menaquinol pool-based origin of electrons that are transferred to CcpA ?
-
?

Organism

Organism UniProt Comment Textmining
Shewanella oneidensis
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant His-tagged CcpA from Escherichia coli strain AS457 Shewanella oneidensis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2,2-azinobis(3-ethylbenzthiazolinesulfonic acid) + H2O2
-
Shewanella oneidensis ?
-
?
additional information menaquinol pool-based origin of electrons that are transferred to CcpA Shewanella oneidensis ?
-
?

Subunits

Subunits Comment Organism
More CcpA three-dimensional structure analysis, overview Shewanella oneidensis

Synonyms

Synonyms Comment Organism
CcpA
-
Shewanella oneidensis
diheme cytochrome c peroxidase
-
Shewanella oneidensis

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
23.1
-
2,2-azinobis(3-ethylbenzthiazolinesulfonic acid) pH 7.5, temperature not specified in the publication Shewanella oneidensis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
assay at Shewanella oneidensis

Cofactor

Cofactor Comment Organism Structure
heme diheme cytochrome c peroxidase Shewanella oneidensis

General Information

General Information Comment Organism
metabolism bacterial diheme c-type cytochrome peroxidases catalyze the periplasmic reduction of hydrogen peroxide to water. CcpA does not seem to be part of a CymAMtrA-FccA-based electron transfer network in the periplasm of Shewanella oneidensis Shewanella oneidensis