Cloned (Comment) | Organism |
---|---|
gene ccpA, expression of His-tagged CcpA in Escherichia coli strain AS457 | Shewanella oneidensis |
Crystallization (Comment) | Organism |
---|---|
purified recombinnat His-tagged CcpA, sitting drop vapor diffusion, mixing of 0.001 ml 7.5 mg/ml dithionite-reduced protein solution with 0.001 ml of reservoir solution containing 26% w/v PEG 2000 monomethyl ether and 0.1 M bis-(2-hydroxyethyl)-amino-tris-(hydroxymethyl)methane, pH 5.0, equilibration against 0.2 ml of reserrvoir solution, 10% v/v (2R,3R)-butanediol as a cryoprotectant, X-ray diffraction structure determination and analysis | Shewanella oneidensis |
Protein Variants | Comment | Organism |
---|---|---|
additional information | construction of a DELTAccpA mutant Shewanella oneidensis line | Shewanella oneidensis |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
2-heptyl-4-hydroxyquinoline N-oxide | - |
Shewanella oneidensis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0113 | - |
2,2-azinobis(3-ethylbenzthiazolinesulfonic acid) | pH 7.5, temperature not specified in the publication | Shewanella oneidensis |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
periplasm | - |
Shewanella oneidensis | - |
- |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Fe2+ | diheme cytochrome c peroxidase | Shewanella oneidensis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Shewanella oneidensis | menaquinol pool-based origin of electrons that are transferred to CcpA | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Shewanella oneidensis | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged CcpA from Escherichia coli strain AS457 | Shewanella oneidensis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2,2-azinobis(3-ethylbenzthiazolinesulfonic acid) + H2O2 | - |
Shewanella oneidensis | ? | - |
? | |
additional information | menaquinol pool-based origin of electrons that are transferred to CcpA | Shewanella oneidensis | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
More | CcpA three-dimensional structure analysis, overview | Shewanella oneidensis |
Synonyms | Comment | Organism |
---|---|---|
CcpA | - |
Shewanella oneidensis |
diheme cytochrome c peroxidase | - |
Shewanella oneidensis |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
23.1 | - |
2,2-azinobis(3-ethylbenzthiazolinesulfonic acid) | pH 7.5, temperature not specified in the publication | Shewanella oneidensis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Shewanella oneidensis |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
heme | diheme cytochrome c peroxidase | Shewanella oneidensis |
General Information | Comment | Organism |
---|---|---|
metabolism | bacterial diheme c-type cytochrome peroxidases catalyze the periplasmic reduction of hydrogen peroxide to water. CcpA does not seem to be part of a CymAMtrA-FccA-based electron transfer network in the periplasm of Shewanella oneidensis | Shewanella oneidensis |