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Literature summary for 1.11.1.5 extracted from

  • Becker, C.F.; Watmough, N.J.; Elliott, S.J.
    Electrochemical evidence for multiple peroxidatic heme states of the diheme cytochrome c peroxidase of Pseudomonas aeruginosa (2009), Biochemistry, 48, 87-95.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli JM109(DE3) with pETCCP coexpressed with pEC86 Pseudomonas aeruginosa

Inhibitors

Inhibitors Comment Organism Structure
cyanide
-
Pseudomonas aeruginosa

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.025
-
H2O2 protein film voltammetry, the midpoint potentials of the turnover signals are used to calculate Michaelis-Menten kinetics Pseudomonas aeruginosa

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ferrocytochrome c + H2O2 Pseudomonas aeruginosa
-
ferricytochrome c + H2O
-
?

Organism

Organism UniProt Comment Textmining
Pseudomonas aeruginosa P14532
-
-

Purification (Commentary)

Purification (Comment) Organism
precipitation, DEAE-Sepharose followed by S-100 size exclusion chromatography Pseudomonas aeruginosa

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ferrocytochrome c + H2O2
-
Pseudomonas aeruginosa ferricytochrome c + H2O
-
?
ferrocytochrome c + H2O2 investigation of the catalytic mechanism Pseudomonas aeruginosa ferricytochrome c + H2O
-
?

Synonyms

Synonyms Comment Organism
BCcP
-
Pseudomonas aeruginosa
cytochrome c peroxidase
-
Pseudomonas aeruginosa
Psa CcP
-
Pseudomonas aeruginosa

pH Range

pH Minimum pH Maximum Comment Organism
3 10 by using the maximum current in the cathodic and anodic halfscans in the course of altering the pH a shift in potential is observed, at pH 7, a single redox couple (I) is dominant, but at more acidic pH values, a second couple (II) is clearly discernible at a more positive value of potential Pseudomonas aeruginosa

pH Stability

pH Stability pH Stability Maximum Comment Organism
3 10 by using the maximum current in the cathodic and anodic halfscans in the course of altering the pH a shift in potential is observed, at pH 7, a single redox couple (I) is dominant, but at more acidic pH values, a second couple (II) is clearly discernible at a more positive value of potential Pseudomonas aeruginosa

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.05
-
cyanide pH 7, 25 microM H2O2, protein film voltammetry, the midpoint potentials of the turnover signals are used to calculate Michaelis-Menten kinetics Pseudomonas aeruginosa