Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli JM109(DE3) with pETCCP coexpressed with pEC86 | Pseudomonas aeruginosa |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
cyanide | - |
Pseudomonas aeruginosa |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.025 | - |
H2O2 | protein film voltammetry, the midpoint potentials of the turnover signals are used to calculate Michaelis-Menten kinetics | Pseudomonas aeruginosa |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ferrocytochrome c + H2O2 | Pseudomonas aeruginosa | - |
ferricytochrome c + H2O | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas aeruginosa | P14532 | - |
- |
Purification (Comment) | Organism |
---|---|
precipitation, DEAE-Sepharose followed by S-100 size exclusion chromatography | Pseudomonas aeruginosa |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ferrocytochrome c + H2O2 | - |
Pseudomonas aeruginosa | ferricytochrome c + H2O | - |
? | |
ferrocytochrome c + H2O2 | investigation of the catalytic mechanism | Pseudomonas aeruginosa | ferricytochrome c + H2O | - |
? |
Synonyms | Comment | Organism |
---|---|---|
BCcP | - |
Pseudomonas aeruginosa |
cytochrome c peroxidase | - |
Pseudomonas aeruginosa |
Psa CcP | - |
Pseudomonas aeruginosa |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
3 | 10 | by using the maximum current in the cathodic and anodic halfscans in the course of altering the pH a shift in potential is observed, at pH 7, a single redox couple (I) is dominant, but at more acidic pH values, a second couple (II) is clearly discernible at a more positive value of potential | Pseudomonas aeruginosa |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
3 | 10 | by using the maximum current in the cathodic and anodic halfscans in the course of altering the pH a shift in potential is observed, at pH 7, a single redox couple (I) is dominant, but at more acidic pH values, a second couple (II) is clearly discernible at a more positive value of potential | Pseudomonas aeruginosa |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.05 | - |
cyanide | pH 7, 25 microM H2O2, protein film voltammetry, the midpoint potentials of the turnover signals are used to calculate Michaelis-Menten kinetics | Pseudomonas aeruginosa |