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Literature summary for 1.11.1.5 extracted from

  • Tano, T.; Sakai, K.; Sugio, T.; Imai, K.
    Purification and some properties of a ferrocytochrome c: hydrogen-peroxide oxidoreductase from Thiobacillus thiooxidans (1977), Agric. Biol. Chem., 41, 323-330.
No PubMed abstract available

Inhibitors

Inhibitors Comment Organism Structure
Ag+ inhibition of NADH oxidizing activity Acidithiobacillus thiooxidans
azide
-
Acidithiobacillus thiooxidans
CO 40% inhibition Acidithiobacillus thiooxidans
Cu2+ inhibition of NADH oxidizing activity Acidithiobacillus thiooxidans
cyanide
-
Acidithiobacillus thiooxidans
Hg2+ inhibition of NADH oxidizing activity Acidithiobacillus thiooxidans
Pb2+ inhibition of NADH oxidizing activity Acidithiobacillus thiooxidans

Metals/Ions

Metals/Ions Comment Organism Structure
Iron heme prosthetic group Acidithiobacillus thiooxidans

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
32000
-
gel filtration Acidithiobacillus thiooxidans

Organism

Organism UniProt Comment Textmining
Acidithiobacillus thiooxidans
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Acidithiobacillus thiooxidans

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
18.16
-
-
Acidithiobacillus thiooxidans

Storage Stability

Storage Stability Organism
-20°C, 0.1 M phosphate buffer, pH 7.0, two months Acidithiobacillus thiooxidans

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ferrocytochrome c + H2O2 horse heart Acidithiobacillus thiooxidans ferricytochrome c + H2O
-
?
ferrocytochrome c + menadione menadione can be substituted by 1,4-naphthoquinone Acidithiobacillus thiooxidans ferricytochrome + oxidized menadione
-
?
NADH + H2O2
-
Acidithiobacillus thiooxidans NAD+ + H2O
-
?
NADPH + H2O2
-
Acidithiobacillus thiooxidans NADP+ + H2O
-
?

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
70
-
15 min, 80% loss of peroxidizing activity, 50% loss of NADH oxidizing activity Acidithiobacillus thiooxidans
90
-
inactivation Acidithiobacillus thiooxidans

pH Stability

pH Stability pH Stability Maximum Comment Organism
2 10
-
Acidithiobacillus thiooxidans