Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Ag+ | inhibition of NADH oxidizing activity | Acidithiobacillus thiooxidans | |
azide | - |
Acidithiobacillus thiooxidans | |
CO | 40% inhibition | Acidithiobacillus thiooxidans | |
Cu2+ | inhibition of NADH oxidizing activity | Acidithiobacillus thiooxidans | |
cyanide | - |
Acidithiobacillus thiooxidans | |
Hg2+ | inhibition of NADH oxidizing activity | Acidithiobacillus thiooxidans | |
Pb2+ | inhibition of NADH oxidizing activity | Acidithiobacillus thiooxidans |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Iron | heme prosthetic group | Acidithiobacillus thiooxidans |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
32000 | - |
gel filtration | Acidithiobacillus thiooxidans |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Acidithiobacillus thiooxidans | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Acidithiobacillus thiooxidans |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
18.16 | - |
- |
Acidithiobacillus thiooxidans |
Storage Stability | Organism |
---|---|
-20°C, 0.1 M phosphate buffer, pH 7.0, two months | Acidithiobacillus thiooxidans |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ferrocytochrome c + H2O2 | horse heart | Acidithiobacillus thiooxidans | ferricytochrome c + H2O | - |
? | |
ferrocytochrome c + menadione | menadione can be substituted by 1,4-naphthoquinone | Acidithiobacillus thiooxidans | ferricytochrome + oxidized menadione | - |
? | |
NADH + H2O2 | - |
Acidithiobacillus thiooxidans | NAD+ + H2O | - |
? | |
NADPH + H2O2 | - |
Acidithiobacillus thiooxidans | NADP+ + H2O | - |
? |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
70 | - |
15 min, 80% loss of peroxidizing activity, 50% loss of NADH oxidizing activity | Acidithiobacillus thiooxidans |
90 | - |
inactivation | Acidithiobacillus thiooxidans |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
2 | 10 | - |
Acidithiobacillus thiooxidans |