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Literature summary for 1.10.3.2 extracted from
- Expression of a thermo- and alkali-philic fungal laccase in Pichia pastoris and its application (2019), Protein Expr. Purif., 154, 16-24 .
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| heterologous expression of lcc9 in Pichia pastoris GS115 | Coprinopsis cinerea |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| copper | copper-containing enzyme | Coprinopsis cinerea |  |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 60200 | - |
MALDI-TOF | Coprinopsis cinerea |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Coprinopsis cinerea | Q08AB8 | - |
- |
| Coprinopsis cinerea ATCC MYA-4618 | Q08AB8 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Coprinopsis cinerea |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 3138 | - |
pH and temperature not specified in the publication, laccase activity in the culture supernatant | Coprinopsis cinerea |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) + O2 | - |
Coprinopsis cinerea | ? | - |
? | |
| 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) + O2 | - |
Coprinopsis cinerea ATCC MYA-4618 | ? | - |
? | |
| acid red 6B + O2 | addition of mediators including methyl syringate, 3,5-dimethoxy-4-hydroxybenzaldehyde, syringic acid, 1-hydroxybenzotriazole, and 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) strongly promote the decolorization rates of the recombinant enzyme rLcc9 on the dyes. Methyl syringate is the best mediator. The decolorization rate of acid red 6B is 98.6% | Coprinopsis cinerea | ? | - |
? | |
| acid red 6B + O2 | addition of mediators including methyl syringate, 3,5-dimethoxy-4-hydroxybenzaldehyde, syringic acid, 1-hydroxybenzotriazole, and 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) strongly promote the decolorization rates of the recombinant enzyme rLcc9 on the dyes. Methyl syringate is the best mediator. The decolorization rate of acid red 6B is 98.6% | Coprinopsis cinerea ATCC MYA-4618 | ? | - |
? | |
| indigo carmine + O2 | addition of mediators including methyl syringate, 3,5-dimethoxy-4-hydroxybenzaldehyde, syringic acid, 1-hydroxybenzotriazole, and 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) strongly promote the decolorization rates of the recombinant enzyme rLcc9 on the dyes. Methyl syringate is the best mediator. The decolorization rate of Indigo carmine is 99.1% | Coprinopsis cinerea | ? | - |
? | |
| indigo carmine + O2 | addition of mediators including methyl syringate, 3,5-dimethoxy-4-hydroxybenzaldehyde, syringic acid, 1-hydroxybenzotriazole, and 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) strongly promote the decolorization rates of the recombinant enzyme rLcc9 on the dyes. Methyl syringate is the best mediator. The decolorization rate of Indigo carmine is 99.1% | Coprinopsis cinerea ATCC MYA-4618 | ? | - |
? | |
| reactive 203 brilliant orange K-7R + O2 | addition of mediators including methyl syringate, 3,5-dimethoxy-4-hydroxybenzaldehyde, syringic acid, 1-hydroxybenzotriazole, and 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) strongly promote the decolorization rates of the recombinant enzyme rLcc9 on the dyes. Methyl syringate is the best mediator. The decolorization rate of reactive 203 brilliant orange K-7R is 94.9% | Coprinopsis cinerea | ? | - |
? | |
| reactive 203 brilliant orange K-7R + O2 | addition of mediators including methyl syringate, 3,5-dimethoxy-4-hydroxybenzaldehyde, syringic acid, 1-hydroxybenzotriazole, and 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) strongly promote the decolorization rates of the recombinant enzyme rLcc9 on the dyes. Methyl syringate is the best mediator. The decolorization rate of reactive 203 brilliant orange K-7R is 94.9% | Coprinopsis cinerea ATCC MYA-4618 | ? | - |
? | |
| reactive blue M-2GE + O2 | addition of mediators including methyl syringate, 3,5-dimethoxy-4-hydroxybenzaldehyde, syringic acid, 1-hydroxybenzotriazole, and 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) strongly promote the decolorization rates of the recombinant enzyme rLcc9 on the dyes. Methyl syringate is the best mediator. The decolorization rate of reactive blue M-2GE is 71.9% | Coprinopsis cinerea | ? | - |
? | |
| reactive brilliant red KD-8B + O2 | addition of mediators including methyl syringate, 3,5-dimethoxy-4-hydroxybenzaldehyde, syringic acid, 1-hydroxybenzotriazole, and 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) strongly promote the decolorization rates of the recombinant enzyme rLcc9 on the dyes. Methyl syringate is the best mediator. The decolorization rate of reactive brilliant red KD-8B is 89.2% | Coprinopsis cinerea | ? | - |
? | |
| reactive brilliant red KM-8B + O2 | addition of mediators including methyl syringate, 3,5-dimethoxy-4-hydroxybenzaldehyde, syringic acid, 1-hydroxybenzotriazole, and 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) strongly promote the decolorization rates of the recombinant enzyme rLcc9 on the dyes. Methyl syringate is the best mediator. The decolorization rate of reactive brilliant red KM-8B is 82.5% | Coprinopsis cinerea | ? | - |
? | |
| syringaldazine + O2 | - |
Coprinopsis cinerea | ? | - |
? | |
| syringaldazine + O2 | - |
Coprinopsis cinerea ATCC MYA-4618 | ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| Lcc9 | - |
Coprinopsis cinerea |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 60 | - |
subatrate: 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) | Coprinopsis cinerea |
| 70 | - |
substrate: syringaldazine | Coprinopsis cinerea |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 60 | - |
pH 4.5, half-life of wild-type enzyme Lcc9 is 24 min, half-life of the recombinant enzyme expressed in Pichia pastoris is 15 min | Coprinopsis cinerea |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 2.5 | - |
subatrate: 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) | Coprinopsis cinerea |
| 6.5 | - |
substrate: syringaldazine | Coprinopsis cinerea |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 6.5 | - |
wild-type enzyme Lcc9 and recombinant enzyme expressed in Pichia pastoris retain about 60% of their original activities | Coprinopsis cinerea |
| 8.5 | - |
1 h, 60°C, wild-type enzyme Lcc9 retains 11.8% of its original activity while the recombinant enzyme expressed in Pichia pastoris retains 63.2% of its original activity | Coprinopsis cinerea |
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