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Literature summary for 1.10.3.2 extracted from

  • Coelho, G.D.; Ballaminut, N.; Thomaz, D.V.; Gomes Machado, K.M.
    Characterization of a thermostable Deconica castanella laccase and application toward pentachlorophenol degradation (2019), Prep. Biochem. Biotechnol., 49, 908-915 .
    View publication on PubMed
Search for more literature for 1.10.3.2

Protein Variants

Protein Variants Comment Organism
degradation the highest biodegradation of the toxic organochlorine pesticide pentachlorophenol (PCP) is of 23% at pollutant concentration of 100 mg/l, which evidences that the thermostable enzyme acts directly in degradation of pentachlorophenol and may be a useful asset to remediate this pollutant Deconica castanella
environmental protection the highest biodegradation of the toxic organochlorine pesticide pentachlorophenol (PCP) is of 23% at pollutant concentration of 100 mg/l, which evidences that the thermostable enzyme acts directly in degradation of pentachlorophenol and may be a useful asset to remediate this pollutant Deconica castanella

Inhibitors

Inhibitors Comment Organism Structure
EDTA 1 mM, 20% inhibition Deconica castanella
FeCl3
-
 Deconica castanella
FeSO4
-
 Deconica castanella
NaN3
-
 Deconica castanella
oxalate 1 mM, 35% inhibition Deconica castanella

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
11.99
-
 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) pH 2.5, 55°C Deconica castanella

Metals/Ions

Metals/Ions Comment Organism Structure
CuSO4 1 mM, 1.1fold activation Deconica castanella
MnSO4 1 mM, 1.2fold activation Deconica castanella

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
64000
-
 gel filtration Deconica castanella

Organism

Organism UniProt Comment Textmining
Deconica castanella
-
-
-
Deconica castanella CCIBt278
-
-
-

Purification (Commentary)

Purification (Comment) Organism
partial Deconica castanella

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) + O2
-
 Deconica castanella ?
-
 ?
2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) + O2
-
 Deconica castanella CCIBt278 ?
-
 ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
55
-
 substrate: 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) Deconica castanella

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
25 70 25°C: about 55% of maximal activity, 75°C: about 75% of maximal activity, substrate: 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) Deconica castanella

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
55
-
 T1/2: 19 h Deconica castanella
60
-
 T1/2: 19 h Deconica castanella
80
-
 T1/2; 47 min Deconica castanella

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
2.5
-
-
 Deconica castanella

pH Stability

pH Stability pH Stability Maximum Comment Organism
2.5
-
 2 h, 22°C, 20% loss of activity Deconica castanella
4
-
 2 h, 22°C, no loss of activity Deconica castanella
5
-
 2 h, 22°C, 5% loss of activity Deconica castanella