Cloned (Comment) | Organism |
---|---|
heterologous expression in Aspergillus niger | Pestalotiopsis sp. KF079 |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.02 | - |
2,6-dimethoxyphenol | 30°C, pH not specified in the publication | Pestalotiopsis sp. KF079 | |
0.024 | - |
2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) | 30°C, pH not specified in the publication | Pestalotiopsis sp. KF079 | |
0.04 | - |
syringaldazine | 30°C, pH not specified in the publication | Pestalotiopsis sp. KF079 | |
0.1 | - |
2,6-dimethoxyphenol | 30°C, pH not specified in the publication | Pestalotiopsis sp. KF079 | |
0.1 | - |
2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) | 30°C, pH not specified in the publication | Pestalotiopsis sp. KF079 | |
0.101 | - |
syringaldazine | 30°C, pH not specified in the publication | Pestalotiopsis sp. KF079 | |
0.144 | - |
o-Dianisidine | 30°C, pH not specified in the publication | Pestalotiopsis sp. KF079 | |
0.647 | - |
o-Dianisidine | 30°C, pH not specified in the publication | Pestalotiopsis sp. KF079 |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
62000 | - |
calculated from sequence | Pestalotiopsis sp. KF079 |
63000 | - |
calculated from sequence | Pestalotiopsis sp. KF079 |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pestalotiopsis sp. KF079 | A0A2U7VJL8 | - |
- |
Pestalotiopsis sp. KF079 | A0A2U7VJY0 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) + O2 | - |
Pestalotiopsis sp. KF079 | ? | - |
? | |
2,6-dimethoxyphenol + O2 | - |
Pestalotiopsis sp. KF079 | ? | - |
? | |
additional information | guaiacol and tannic acid are poorly oxidized. Ferulic and or 3,4-dihydroxyphenylalanine (DOPA) are not oxidized at all. The industrially important dyes Acid Yellow, Bromo Cresol Purple, Nitrosulfonazo III, and Reactive Black 5 are more effciently decolorized by PsLac1 in the presence of the redox mediator 1-hydroxybenzotriazole | Pestalotiopsis sp. KF079 | ? | - |
- |
|
o-dianisidine + O2 | - |
Pestalotiopsis sp. KF079 | ? | - |
? | |
syringaldazine + O2 | - |
Pestalotiopsis sp. KF079 | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
PsLac1 | - |
Pestalotiopsis sp. KF079 |
PsLac2 | - |
Pestalotiopsis sp. KF079 |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
substrate: 2,6-dimethoxyphenol | Pestalotiopsis sp. KF079 |
55 | - |
substrate: syringaldazine | Pestalotiopsis sp. KF079 |
65 | - |
substrate: syringaldazine | Pestalotiopsis sp. KF079 |
70 | - |
substrated: 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) | Pestalotiopsis sp. KF079 |
80 | - |
substrate: 2,6-dimethoxyphenol | Pestalotiopsis sp. KF079 |
80 | - |
substrated: 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) | Pestalotiopsis sp. KF079 |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
20 | 80 | 20°C: about 70% of maximal activity, 80°C: about 40% of maximal activity, substrate: 2,6-dimethoxyphenol | Pestalotiopsis sp. KF079 |
20 | 80 | 20°C: about 70% of maximal activity, 80°C: about 40% of maximal activity, substrated: 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) | Pestalotiopsis sp. KF079 |
35 | 60 | 35°C: about 30% of maximal activity, 60°C: about 35% of maximal activity, substrate: syringaldazine | Pestalotiopsis sp. KF079 |
40 | 80 | 40°C: about 40% of maximal activity, 80°C: maximal activity, substrate: 2,6-dimethoxyphenol | Pestalotiopsis sp. KF079 |
45 | 75 | 45°C: about 45% of maximal activity, 75°C: about 55% of maximal activity, substrate: syringaldazine | Pestalotiopsis sp. KF079 |
50 | 80 | 50°C: about 50% of maximal activity, 80°C: maximal activity, substrated: 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) | Pestalotiopsis sp. KF079 |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
the enzyme retains 100% activity after 3 h | Pestalotiopsis sp. KF079 |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.057 | - |
o-Dianisidine | 30°C, pH not specified in the publication | Pestalotiopsis sp. KF079 | |
0.178 | - |
2,6-dimethoxyphenol | 30°C, pH not specified in the publication | Pestalotiopsis sp. KF079 | |
0.365 | - |
syringaldazine | 30°C, pH not specified in the publication | Pestalotiopsis sp. KF079 | |
0.431 | - |
2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) | 30°C, pH not specified in the publication | Pestalotiopsis sp. KF079 | |
0.573 | - |
syringaldazine | 30°C, pH not specified in the publication | Pestalotiopsis sp. KF079 | |
0.696 | - |
2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) | 30°C, pH not specified in the publication | Pestalotiopsis sp. KF079 | |
0.748 | - |
o-Dianisidine | 30°C, pH not specified in the publication | Pestalotiopsis sp. KF079 | |
2.441 | - |
2,6-dimethoxyphenol | 30°C, pH not specified in the publication | Pestalotiopsis sp. KF079 |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
2 | - |
substrated: 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) | Pestalotiopsis sp. KF079 |
3 | - |
substrated: 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) | Pestalotiopsis sp. KF079 |
5 | - |
substrate: 2,6-dimethoxyphenol | Pestalotiopsis sp. KF079 |
5 | - |
substrate: syringaldazine | Pestalotiopsis sp. KF079 |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
4 | 6 | the enzyme is stable | Pestalotiopsis sp. KF079 |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Pestalotiopsis sp. KF079 | calculated from sequence | - |
4.13 |
Pestalotiopsis sp. KF079 | calculated from sequence | - |
6.17 |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.089 | - |
o-Dianisidine | 30°C, pH not specified in the publication | Pestalotiopsis sp. KF079 | |
4.331 | - |
2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) | 30°C, pH not specified in the publication | Pestalotiopsis sp. KF079 | |
5.187 | - |
o-Dianisidine | 30°C, pH not specified in the publication | Pestalotiopsis sp. KF079 | |
5.672 | - |
syringaldazine | 30°C, pH not specified in the publication | Pestalotiopsis sp. KF079 | |
9.102 | - |
2,6-dimethoxyphenol | 30°C, pH not specified in the publication | Pestalotiopsis sp. KF079 | |
24.532 | - |
2,6-dimethoxyphenol | 30°C, pH not specified in the publication | Pestalotiopsis sp. KF079 | |
29.245 | - |
2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) | 30°C, pH not specified in the publication | Pestalotiopsis sp. KF079 | |
82.936 | - |
syringaldazine | 30°C, pH not specified in the publication | Pestalotiopsis sp. KF079 |