Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli BL21(DE3) | Thermus thermophilus |
Protein Variants | Comment | Organism |
---|---|---|
D394E | mutant with the lower laccase activity displays a decreased decolorization efficiency as compared to the wild-type enzyme. Expressed in a lower level, about 50%, of the wild type enzyme. Optimum pH shifts towards the acidic value (0.5-1 units) relative to the wild type enzyme which has an optimal pH 6.0 | Thermus thermophilus |
D394M | mutant with the lower laccase activity displays a decreased decolorization efficiency as compared to the wild-type enzyme. Expressed in a lower level, about 50%, of the wild type enzyme. Optimum pH shifts towards the acidic value (0.5-1 units) relative to the wild type enzyme which has an optimal pH 6.0 | Thermus thermophilus |
D394R | mutant with the lower laccase activity displays a decreased decolorization efficiency as compared to the wild-type enzyme. Expressed in a lower level, about 16%, of the wild type enzyme. Optimum pH shifts towards the acidic value (0.5-1 units) relative to the wild type enzyme which has an optimal pH 6.0 | Thermus thermophilus |
D396A | mutant enzyme with higher catalytic efficiency decolorizes the synthetic dye more efficiently than the wild-type enzyme | Thermus thermophilus |
D396E | mutant enzyme with higher catalytic efficiency decolorizes the synthetic dye more efficiently than the wild-type enzyme | Thermus thermophilus |
D396M | mutant enzyme with higher catalytic efficiency decolorizes the synthetic dye more efficiently than the wild-type enzyme | Thermus thermophilus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.215 | - |
guaiacol | pH 6.0, 90°C, mutant enzyme D394M | Thermus thermophilus | |
0.243 | - |
guaiacol | pH 6.0, 90°C, mutant enzyme D394R | Thermus thermophilus | |
0.266 | - |
guaiacol | pH 6.0, 90°C, mutant enzyme D394E | Thermus thermophilus | |
0.325 | - |
guaiacol | pH 6.0, 90°C, mutant enzyme D396A | Thermus thermophilus | |
0.356 | - |
guaiacol | pH 6.0, 90°C, mutant enzyme D396M | Thermus thermophilus | |
0.382 | - |
guaiacol | pH 6.0, 90°C, mutant enzyme D396E | Thermus thermophilus | |
0.392 | - |
guaiacol | pH 6.0, 90°C, mutant enzyme D396N | Thermus thermophilus | |
0.407 | - |
guaiacol | pH 6.0, 90°C, wild-type enzyme | Thermus thermophilus | |
0.439 | - |
guaiacol | pH 6.0, 90°C, mutant enzyme D394N | Thermus thermophilus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
copper | multicopper oxidase | Thermus thermophilus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Thermus thermophilus | F6DF14 | - |
- |
Thermus thermophilus SG0.5JP17-16 | F6DF14 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Thermus thermophilus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
Congo Red + O2 | the enzyme decolorizes the azo dye | Thermus thermophilus | ? | - |
? | |
Congo Red + O2 | the enzyme decolorizes the azo dye | Thermus thermophilus SG0.5JP17-16 | ? | - |
? | |
guaiacol + O2 | - |
Thermus thermophilus | 6-methoxycyclohexa-2,4-dienone + H2O | - |
? | |
guaiacol + O2 | - |
Thermus thermophilus SG0.5JP17-16 | 6-methoxycyclohexa-2,4-dienone + H2O | - |
? | |
Reactive Black B + O2 | the enzyme decolorizes the azo dye | Thermus thermophilus | ? | - |
? | |
Reactive Black B + O2 | the enzyme decolorizes the azo dye | Thermus thermophilus SG0.5JP17-16 | ? | - |
? | |
Reactive Black WNN + O2 | the enzyme decolorizes the azo dye | Thermus thermophilus | ? | - |
? | |
Reactive Black WNN + O2 | the enzyme decolorizes the azo dye | Thermus thermophilus SG0.5JP17-16 | ? | - |
? | |
Remazol Brilliant Blue R + O2 | the enzyme decolorizes the anthraquinone dye | Thermus thermophilus | ? | - |
? | |
Remazol Brilliant Blue R + O2 | the enzyme decolorizes the anthraquinone dye | Thermus thermophilus SG0.5JP17-16 | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
lacTT | - |
Thermus thermophilus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
90 | - |
wild-type enzyme and mutant enzymes D394E, D394M and D394R | Thermus thermophilus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
80 | - |
4 h, wild-type enzymed retains 80% of its activity. The thermal stability of D394R mutant decreases significantly, with the residual activity of 15% after 4 h | Thermus thermophilus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.8 | - |
guaiacol | pH 6.0, 90°C, mutant enzyme D394R | Thermus thermophilus | |
1.7 | - |
guaiacol | pH 6.0, 90°C, mutant enzyme D394M | Thermus thermophilus | |
3.3 | - |
guaiacol | pH 6.0, 90°C, mutant enzyme D394E | Thermus thermophilus | |
6.7 | - |
guaiacol | pH 6.0, 90°C, mutant enzyme D396N | Thermus thermophilus | |
6.9 | - |
guaiacol | pH 6.0, 90°C, wild-type enzyme | Thermus thermophilus | |
7.1 | - |
guaiacol | pH 6.0, 90°C, mutant enzyme D396A | Thermus thermophilus | |
7.3 | - |
guaiacol | pH 6.0, 90°C, mutant enzyme D396M | Thermus thermophilus | |
7.4 | - |
guaiacol | pH 6.0, 90°C, mutant enzyme D394N | Thermus thermophilus | |
7.4 | - |
guaiacol | pH 6.0, 90°C, mutant enzyme D396E | Thermus thermophilus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6 | - |
wild-type enzyme | Thermus thermophilus |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
3.3 | - |
guaiacol | pH 6.0, 90°C, mutant enzyme D394R | Thermus thermophilus | |
7.9 | - |
guaiacol | pH 6.0, 90°C, mutant enzyme D394M | Thermus thermophilus | |
12.4 | - |
guaiacol | pH 6.0, 90°C, mutant enzyme D394E | Thermus thermophilus | |
16.8 | - |
guaiacol | pH 6.0, 90°C, mutant enzyme D394N | Thermus thermophilus | |
16.9 | - |
guaiacol | pH 6.0, 90°C, wild-type enzyme | Thermus thermophilus | |
17.1 | - |
guaiacol | pH 6.0, 90°C, mutant enzyme D396N | Thermus thermophilus | |
19.4 | - |
guaiacol | pH 6.0, 90°C, mutant enzyme D396E | Thermus thermophilus | |
20.5 | - |
guaiacol | pH 6.0, 90°C, mutant enzyme D396M | Thermus thermophilus | |
21.8 | - |
guaiacol | pH 6.0, 90°C, mutant enzyme D396A | Thermus thermophilus |