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Literature summary for 1.10.3.2 extracted from

  • Wang, B.; Wang, L.; Lin, Y.; Han, Q.; Han, J.; Gao, J.; Tian, Y.; Zhao, W.; Peng, R.; Yao, Q.
    Purification and characterization of a laccase from Coprinopsis cinerea in Pichia pastoris (2014), World J. Microbiol. Biotechnol., 30, 1199-1206 .
    View publication on PubMed
Search for more literature for 1.10.3.2

Cloned(Commentary)

Cloned (Comment) Organism
expression in Pichia pastoris Coprinopsis cinerea

Inhibitors

Inhibitors Comment Organism Structure
Al3+ strongly inhibits at 10 mM Coprinopsis cinerea
Fe3+ strongly inhibits at 10 mM Coprinopsis cinerea
Mn2+ strongly inhibits at 10 mM Coprinopsis cinerea
SDS 1 mM, 98% inhibition Coprinopsis cinerea
sodium thiosulfate 1 mM, 98% inhibition Coprinopsis cinerea
Zn2+ strongly inhibits at 10 mM Coprinopsis cinerea

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.149
-
 2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) pH 3.0, 37°C Coprinopsis cinerea
1.5 2 2,6-dimethoxyphenol pH 3.0, 37°C Coprinopsis cinerea

Organism

Organism UniProt Comment Textmining
Coprinopsis cinerea Q08AC1
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Coprinopsis cinerea

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) + O2
-
 Coprinopsis cinerea ?
-
 ?
2,6-dimethoxyphenol + O2
-
 Coprinopsis cinerea ?
-
 ?
additional information 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid), as a mediator, is necessary in decolorizing Malachite green Coprinopsis cinerea ?
-
 ?

Subunits

Subunits Comment Organism
? 1 * 57400, SDS-PAGE Coprinopsis cinerea

Synonyms

Synonyms Comment Organism
CcLCC6
-
 Coprinopsis cinerea

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
40
-
-
 Coprinopsis cinerea

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
20 60 20°C: about 60% of maximal activity, 60°C: about 50% of maximal activity Coprinopsis cinerea

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
60
-
 the stability of laccase is not affected by temperatures below 60°C Coprinopsis cinerea
80
-
 the remaining activity is 37% and 7% following treatment for 10 min and 60 min Coprinopsis cinerea

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.3
-
 2,6-dimethoxyphenol pH 3.0, 37°C Coprinopsis cinerea
32.03
-
 2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) pH 3.0, 37°C Coprinopsis cinerea

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
3
-
-
 Coprinopsis cinerea

pH Range

pH Minimum pH Maximum Comment Organism
2.3 4 pH 2.3: about 40% of maximal activity, pH 4.0: about 75% of maximal activity Coprinopsis cinerea

pH Stability

pH Stability pH Stability Maximum Comment Organism
3 5.6 after treatment with citrate-phosphate buffer (pH 3.0-5.6) for 24 h, enzyme activity is not lost Coprinopsis cinerea