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Literature summary for 1.10.3.2 extracted from

  • Campos, P.; Levin, L.; Wirth, S.
    Heterologous production, characterization and dye decolorization ability of a novel thermostable laccase isoenzyme from Trametes trogii BAFC 463 (2016), Process Biochem., 51, 895-903 .
No PubMed abstract available
Search for more literature for 1.10.3.2

Application

Application Comment Organism
environmental protection laccase can be efficiently used to decolorize synthetic dye and is a suitable candidate for the treatment of wastewater from industrial effluents Coriolopsis trogii

Cloned(Commentary)

Cloned (Comment) Organism
expression of lcc3 gene in Komagataella (i.e. Pichia) pastoris Coriolopsis trogii

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.25
-
 2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) pH 4, 30°C Coriolopsis trogii
2.095
-
 2,6-dimethoxyphenol pH 4, 30°C Coriolopsis trogii

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular
-
 Coriolopsis trogii
-
-

Metals/Ions

Metals/Ions Comment Organism Structure
Cu2+ multicopper enzyme Coriolopsis trogii

Organism

Organism UniProt Comment Textmining
Coriolopsis trogii A0A140CWW4
-
-
Coriolopsis trogii BAFC 463 A0A140CWW4
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) + O2
-
 Coriolopsis trogii ?
-
 ?
2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) + O2
-
 Coriolopsis trogii BAFC 463 ?
-
 ?
2,6-dimethoxyphenol + O2
-
 Coriolopsis trogii ?
-
 ?
2,6-dimethoxyphenol + O2
-
 Coriolopsis trogii BAFC 463 ?
-
 ?
additional information the enzyme is capable of decolorizing between 50% and 100% of indigoid, triarylmethane, azoic and anthraquinonic synthetic dyes in the presence of the natural redox mediator acetosyringone within 2 h of incubation at pH 6 and 70°C Coriolopsis trogii ?
-
 ?
additional information the enzyme is capable of decolorizing between 50% and 100% of indigoid, triarylmethane, azoic and anthraquinonic synthetic dyes in the presence of the natural redox mediator acetosyringone within 2 h of incubation at pH 6 and 70°C Coriolopsis trogii BAFC 463 ?
-
 ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
50
-
-
 Coriolopsis trogii

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
40 75 maximum activity at 50°C, retaining more than 80% of activity at temperatures between 60°C and 75°C, but less than 55% of activity at temperatures below 40°C Coriolopsis trogii

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
60
-
 the enzyme retains 100% of its initial activity after 3 h of incubation Coriolopsis trogii
70
-
 half-life: 45 min Coriolopsis trogii

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
329
-
 2,6-dimethoxyphenol pH 4, 30°C Coriolopsis trogii
399
-
 2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) pH 4, 30°C Coriolopsis trogii

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
3.5
-
 substrate: 2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) Coriolopsis trogii
4
-
 substrate: 2,6-dimethoxyphenol Coriolopsis trogii

pH Stability

pH Stability pH Stability Maximum Comment Organism
6 7 25°C, no decrease of activity for at least 5 days Coriolopsis trogii

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
157
-
 2,6-dimethoxyphenol pH 4, 30°C Coriolopsis trogii
1596
-
 2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) pH 4, 30°C Coriolopsis trogii