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Literature summary for 1.10.3.2 extracted from
- Purification and characterization of an extracellular, thermo-alkali-stable, metal tolerant laccase from Bacillus tequilensis SN4 (2014), PLoS ONE, 9, e96951 .
General Stability
| General Stability | Organism |
|---|---|
| the enzyme is stable in the presence of cationic and anionic detergents. In the presence of non-ionic detergents, the enzyme is stable at lower concentrations | Bacillus tequilensis |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 2-mercaptoethanol | 1 mM, complete inactivation | Bacillus tequilensis |  |
| cysteine | 5 mM, 92% loss of activity | Bacillus tequilensis | |
| Diethylenetriaminepentaacetic acid | 10 mM, 76% loss of activity | Bacillus tequilensis | |
| dithiothreitol | 5 mM, complete inactivation. 1 mM, 80% loss of activity | Bacillus tequilensis | |
| EDTA | 10 mM, 77% loss of activity | Bacillus tequilensis | |
| Fe2+ | 5 mM, 59% loss of activity | Bacillus tequilensis | |
| Hg2+ | 1.0 mM, 48% loss of activity | Bacillus tequilensis | |
| Li+ | 5 mM, 22% loss of activity | Bacillus tequilensis | |
| Mg2+ | 5 mM, 23% loss of activity | Bacillus tequilensis | |
| Mn2+ | 5 mM, 21% loss of activity | Bacillus tequilensis | |
| NaN3 | 10 mM, 68% loss of activity | Bacillus tequilensis | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.84 | - |
2,6-dimethoxyphenol | pH 8.0, 85°C | Bacillus tequilensis | |
| 1.4 | - |
2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) | pH 8.0, 85°C | Bacillus tequilensis | |
| 3.26 | - |
guaiacol | pH 8.0, 85°C | Bacillus tequilensis | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| extracellular | - |
Bacillus tequilensis | - |
- |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Co2+ | 5.0 mM, increased activity to 150% | Bacillus tequilensis | |
| Cu2+ | 5.0 mM, increased activity to 126% | Bacillus tequilensis | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bacillus tequilensis | - |
- |
- |
| Bacillus tequilensis SN4 | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Bacillus tequilensis |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) + O2 | - |
Bacillus tequilensis | ? | - |
? | |
| 2,6-dimethoxyphenol + O2 | - |
Bacillus tequilensis | ? | - |
? | |
| alpha-naphthol + O2 | - |
Bacillus tequilensis | ? | - |
? | |
| catechol + O2 | - |
Bacillus tequilensis | ? | - |
? | |
| catechol + O2 | - |
Bacillus tequilensis SN4 | ? | - |
? | |
| guaiacol + O2 | - |
Bacillus tequilensis | ? | - |
? | |
| L-DOPA + O2 | - |
Bacillus tequilensis | ? | - |
? | |
| additional information | poorly oxidizes m-phenols (resorcinol) and shows no activity towards tyrosine | Bacillus tequilensis | ? | - |
? | |
| additional information | poorly oxidizes m-phenols (resorcinol) and shows no activity towards tyrosine | Bacillus tequilensis SN4 | ? | - |
? | |
| p-phenylenediamine + O2 | - |
Bacillus tequilensis | ? | - |
? | |
| p-phenylenediamine + O2 | - |
Bacillus tequilensis SN4 | ? | - |
? | |
| pyrogallol + O2 | - |
Bacillus tequilensis | ? | - |
? | |
| pyrogallol + O2 | - |
Bacillus tequilensis SN4 | ? | - |
? | |
| syringaldazine + O2 | - |
Bacillus tequilensis | ? | - |
? | |
| syringaldazine + O2 | - |
Bacillus tequilensis SN4 | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 32000, SDS-PAGE | Bacillus tequilensis |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| SN4LAC | - |
Bacillus tequilensis |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 85 | - |
- |
Bacillus tequilensis |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 65 | 100 | 65°C: about 50% of maximal activity, 100°C: about 50% of maximal activity | Bacillus tequilensis |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 65 | - |
24 h, completely stable | Bacillus tequilensis |
| 75 | - |
half-life: 4 h | Bacillus tequilensis |
| 80 | - |
half-life: 3 h | Bacillus tequilensis |
| 85 | - |
half-life: 1 h | Bacillus tequilensis |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 62.96 | - |
guaiacol | pH 8.0, 85°C | Bacillus tequilensis | |
| 67.04 | - |
2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) | pH 8.0, 85°C | Bacillus tequilensis | |
| 73.15 | - |
2,6-dimethoxyphenol | pH 8.0, 85°C | Bacillus tequilensis | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5.5 | - |
substrate: 2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) | Bacillus tequilensis |
| 6.5 | - |
substrate: syringaldazine | Bacillus tequilensis |
| 8 | - |
substrate: 2,6-dimethoxyphenol | Bacillus tequilensis |
| 8 | - |
substrate: guaiacol | Bacillus tequilensis |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 9 | - |
24 h, the enzyme retains 75% of its activity | Bacillus tequilensis |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 19.34 | - |
guaiacol | pH 8.0, 85°C | Bacillus tequilensis | |
| 47.82 | - |
2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) | pH 8.0, 85°C | Bacillus tequilensis | |
| 87.05 | - |
2,6-dimethoxyphenol | pH 8.0, 85°C | Bacillus tequilensis | |
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