Application | Comment | Organism |
---|---|---|
degradation | the enzyme is potentially useful for industrial and environmental applications such as textile finishing and wastewater treatment. It decolorizes structurally different dyes and a real textile effluent | Cerrena sp. |
environmental protection | the enzyme is potentially useful for industrial and environmental applications such as textile finishing and wastewater treatment. It decolorizes structurally different dyes and a real textile effluent | Cerrena sp. |
Cloned (Comment) | Organism |
---|---|
- |
Cerrena sp. |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
dithiothreitol | 0.1 mM, activity is reduced to 5.9% | Cerrena sp. | |
Fe2+ | 10 mM, 94% loss of activity | Cerrena sp. | |
kojic acid | 12.5 mM, about 50% loss of activity | Cerrena sp. | |
L-cysteine | 0.1 mM, activity is reduced to 5.5% | Cerrena sp. | |
Li+ | 10 mM, 51% loss of activity | Cerrena sp. | |
additional information | no significant loss of activity loss at 10 mM EDTA | Cerrena sp. | |
SDS | 0.1 mM, activity is reduced to 11.4% | Cerrena sp. | |
Sodium azide | 0.1 mM, activity is reduced to 9.2% | Cerrena sp. |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0934 | - |
2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) | pH and temperature not specified in the publication | Cerrena sp. | |
0.2 | - |
2,6-dimethoxyphenol | pH and temperature not specified in the publication | Cerrena sp. | |
0.3 | - |
guaiacol | pH and temperature not specified in the publication | Cerrena sp. | |
0.529 | - |
catechol | pH and temperature not specified in the publication | Cerrena sp. |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | slightly stimulatory | Cerrena sp. | |
K+ | slightly stimulatory | Cerrena sp. | |
Mg2+ | slightly stimulatory | Cerrena sp. | |
Mn2+ | slightly stimulatory | Cerrena sp. | |
Na+ | slightly stimulatory | Cerrena sp. | |
Zn2+ | slightly stimulatory | Cerrena sp. |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
58600 | - |
- |
Cerrena sp. |
Organic Solvent | Comment | Organism |
---|---|---|
acetonitrile | 25% (v/v), 4 h, 25°C, no loss of activity | Cerrena sp. |
dimethylformamide | 25% (v/v),4 h, 25°C, no loss of activity | Cerrena sp. |
DMSO | 25% (v/v), 4 h, 25°C, no loss of activity | Cerrena sp. |
Ethanol | 25% (v/v), 4 h, 25°C, no loss of activity | Cerrena sp. |
Methanol | 25% (v/v), 4 h, 25°C, no loss of activity | Cerrena sp. |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Cerrena sp. | A0A0A0Q5C7 | - |
- |
Cerrena sp. HYB07 | A0A0A0Q5C7 | - |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
glycoprotein | 7.2% carbohydrate | Cerrena sp. |
Purification (Comment) | Organism |
---|---|
- |
Cerrena sp. |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
mycelium | - |
Cerrena sp. | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
1.95 | - |
pH and temperature not specified in the publication | Cerrena sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) + O2 | catalytic efficiency on 2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) is 7.5 times of that on 2,6-dimethoxyphenol, 22 times of that on catechol and 65times higher than that on guaiacol | Cerrena sp. | ? | - |
? | |
2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) + O2 | catalytic efficiency on 2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) is 7.5 times of that on 2,6-dimethoxyphenol, 22 times of that on catechol and 65times higher than that on guaiacol | Cerrena sp. HYB07 | ? | - |
? | |
2,6-dimethoxyphenol + O2 | - |
Cerrena sp. | ? | - |
? | |
2,6-dimethoxyphenol + O2 | - |
Cerrena sp. HYB07 | ? | - |
? | |
catechol + O2 | - |
Cerrena sp. | ? | - |
? | |
catechol + O2 | - |
Cerrena sp. HYB07 | ? | - |
? | |
guaiacol + O2 | - |
Cerrena sp. | ? | - |
? | |
guaiacol + O2 | - |
Cerrena sp. HYB07 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 58600, SDS-PAGE | Cerrena sp. |
Synonyms | Comment | Organism |
---|---|---|
LacA | - |
Cerrena sp. |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
45 | - |
substrate: 2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) | Cerrena sp. |
45 | 50 | substrate: guaiacol or catechol | Cerrena sp. |
50 | - |
substrate: 2,6-dimethoxyphenol | Cerrena sp. |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | 65 | the enzyme displays more than 50% of maximal activity between 25°C and 65°C against guaiacol, catechol or 2,6-dimethoxyphenol. At 65°C for 2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid), the relative activity of LacA is 45% | Cerrena sp. |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
150 min, 85% of the original enzyme activity is retained | Cerrena sp. |
60 | - |
150 min, 56% of the original enzyme activity is retained | Cerrena sp. |
70 | - |
inactivated after 20 min | Cerrena sp. |
80 | - |
inactivated after 2 min | Cerrena sp. |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
128.1 | - |
guaiacol | pH and temperature not specified in the publication | Cerrena sp. | |
626 | - |
catechol | pH and temperature not specified in the publication | Cerrena sp. | |
703.7 | - |
2,6-dimethoxyphenol | pH and temperature not specified in the publication | Cerrena sp. | |
2468 | - |
2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) | pH and temperature not specified in the publication | Cerrena sp. |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
3 | - |
substrate: 2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) or 2,6-dimethoxyphenol | Cerrena sp. |
4 | - |
substrate: guaiacol | Cerrena sp. |
4.5 | - |
substrate: catechol | Cerrena sp. |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
2 | - |
activity decreases to approximately 1% at the end of 105 h incubation. t1/2: 18 h | Cerrena sp. |
3 | - |
activity decreases to approximately 20% at the end of 105 h incubation. t1/2: 43 h | Cerrena sp. |
4 | - |
105 h, 56% residual enzyme activity is retained | Cerrena sp. |
5 | - |
or above, 105 h, more than 80% residual enzyme activity is retained | Cerrena sp. |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
400 | - |
guaiacol | pH and temperature not specified in the publication | Cerrena sp. | |
1200 | - |
catechol | pH and temperature not specified in the publication | Cerrena sp. | |
2642 | - |
2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) | pH and temperature not specified in the publication | Cerrena sp. | |
3500 | - |
2,6-dimethoxyphenol | pH and temperature not specified in the publication | Cerrena sp. |