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Literature summary for 1.10.3.2 extracted from
- Enzymological characterization of atm, the first laccase from Agrobacterium sp. S5-1, with the ability to enhance in vitro digestibility of maize Straw (2015), PLoS ONE, 10, e0128204 .
Application
| Application | Comment | Organism |
|---|---|---|
| biofuel production | potential for bioconversion of lignin rich agricultural byproducts into animal feed and cellulosic ethanol. The enzyme effectively improves in vitro digestibility of maize straw | Agrobacterium sp. S5-1 |
| food industry | potential for bioconversion of lignin rich agricultural byproducts into animal feed and cellulosic ethanol. The enzyme effectively improves in vitro digestibility of maize straw | Agrobacterium sp. S5-1 |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| heterologously expressed in Escherichia coli | Agrobacterium sp. S5-1 |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Ba2+ | partially inhibits laccase activity | Agrobacterium sp. S5-1 |  |
| Co3+ | 20 mM, weak inhibitory effect | Agrobacterium sp. S5-1 | |
| dithiothreitol | - |
Agrobacterium sp. S5-1 | |
| EDTA | 5 mM or 20 mM, partially inhibits activity | Agrobacterium sp. S5-1 | |
| Fe2+ | partially inhibits laccase activity | Agrobacterium sp. S5-1 | |
| Fe3+ | partially inhibits laccase activity | Agrobacterium sp. S5-1 | |
| additional information | Mn2+ even at 20 mM does not affect Atm activity | Agrobacterium sp. S5-1 | |
| Ni2+ | 20 mM, weak inhibitory effect | Agrobacterium sp. S5-1 | |
| SDS | - |
Agrobacterium sp. S5-1 | |
| Sodium azide | - |
Agrobacterium sp. S5-1 | |
| Zn2+ | partially inhibits laccase activity | Agrobacterium sp. S5-1 | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.2308 | - |
2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) | 30°C, pH 4.5 | Agrobacterium sp. S5-1 | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Cu2+ | activity is low in the absence of Cu2+, so 1 mM Cu2+ is added into the assay buffer | Agrobacterium sp. S5-1 | |
Organic Solvent Stability
| Organic Solvent | Comment | Organism |
|---|---|---|
| Acetone | 10% v/v, 10°C, 1 h, 100% of activity remains | Agrobacterium sp. S5-1 |
| dimethyl sulfoxide | 10% v/v, 10°C, 1 h, 72% of activity remains | Agrobacterium sp. S5-1 |
| Ethanol | 10% v/v, 10°C, 1 h, 84% of activity remains | Agrobacterium sp. S5-1 |
| Methanol | 10% v/v, 10°C, 1 h, 95% of activity remains | Agrobacterium sp. S5-1 |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Agrobacterium sp. S5-1 | A0A0F6SIA9 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant enzyme | Agrobacterium sp. S5-1 |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 185.4 | - |
30°C, pH 4.5, substrate: 2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) | Agrobacterium sp. S5-1 |
Storage Stability
| Storage Stability | Organism |
|---|---|
| 4°C, accase retained more than 50% activity in the phosphate-buffered saline, stable for 1 week | Agrobacterium sp. S5-1 |
| 4°C, in deionized water, stable for more than 20 days | Agrobacterium sp. S5-1 |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) + O2 | - |
Agrobacterium sp. S5-1 | ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| ATM | - |
Agrobacterium sp. S5-1 |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | - |
- |
Agrobacterium sp. S5-1 |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 45 | 60 | 45°C: about 20% of maximal activity, 60°C: about 25% of maximal activity | Agrobacterium sp. S5-1 |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 40 | - |
half-life time (t1/s): more than 12 h | Agrobacterium sp. S5-1 |
| 70 | - |
half-life time (t1/s): 30 min | Agrobacterium sp. S5-1 |
| 80 | - |
10 min, complete inactivation | Agrobacterium sp. S5-1 |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 32.8 | - |
2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) | 30°C, pH 4.5 | Agrobacterium sp. S5-1 | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 4.5 | - |
- |
Agrobacterium sp. S5-1 |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 2 | 6 | pH 2.0: about 30% of maximal activity, pH 6.0: about 30% of maximal activity | Agrobacterium sp. S5-1 |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 2 | - |
30°C, 40% of enzymatic activities after 1 h treatment | Agrobacterium sp. S5-1 |
| 9 | - |
30°C, 5.5% of enzymatic activities after 1 h treatment | Agrobacterium sp. S5-1 |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 142.1 | - |
2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) | 30°C, pH 4.5 | Agrobacterium sp. S5-1 | |
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