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Literature summary for 1.10.3.2 extracted from

  • Ai, M.Q.; Wang, F.F.; Huang, F.
    Purification and characterization of a thermostable laccase from Trametes trogii and its ability in modification of Kraft lignin (2015), J. Microbiol. Biotechnol., 25, 1361-1370 .
    View publication on PubMed
Search for more literature for 1.10.3.2

Application

Application Comment Organism
industry potential use of the laccase in lignin modification Coriolopsis trogii
synthesis potential use of the laccase in lignin modification Coriolopsis trogii

Inhibitors

Inhibitors Comment Organism Structure
AlCl3 10 mM, 40% inhibition Coriolopsis trogii
CaCl2 10 mM, 31% inhibition Coriolopsis trogii
CoSO4 10 mM, 9% inhibition Coriolopsis trogii
CuSO4 10 mM, 19% inhibition Coriolopsis trogii
dithiothreitol 0.1 mM, complete inhibition Coriolopsis trogii
EDTA 10 mM, 6% inhibition Coriolopsis trogii
FeCl3 5 mM, complete inhibition Coriolopsis trogii
KCl 10 mM, 21% inhibition Coriolopsis trogii
KI 5 mM, 25% inhibition Coriolopsis trogii
MgSO4 10 mM, 6% inhibition Coriolopsis trogii
MnSO4 10 mM, 8% inhibition Coriolopsis trogii
NaCl 10 mM, 17% inhibition Coriolopsis trogii
NaF 0.1 mM, 75% inhibition Coriolopsis trogii
NaN3 0.1 mM, complete inhibition Coriolopsis trogii
SDS 5 mM, 46% inhibition Coriolopsis trogii
ZnSO4 10 mM, 0.5% inhibition Coriolopsis trogii

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.00673
-
 syringaldazine pH 4.0, 25°C Coriolopsis trogii
0.00732
-
 2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) pH 4.0, 25°C Coriolopsis trogii
0.302
-
 2,6-dimethoxyphenol pH 4.0, 25°C Coriolopsis trogii
0.552
-
 guaiacol pH 4.0, 25°C Coriolopsis trogii

Metals/Ions

Metals/Ions Comment Organism Structure
Cu2+ contains a type III binuclear Cu2+ center and a type I Cu2+ center Coriolopsis trogii

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
54000
-
 gel filtration Coriolopsis trogii

Organism

Organism UniProt Comment Textmining
Coriolopsis trogii
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Coriolopsis trogii

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
9.1
-
 substrate: poyrogallol, pH 4.0, 25°C Coriolopsis trogii
13.2
-
 substrate: catechol, pH 4.0, 25°C Coriolopsis trogii
15.8
-
 substrate: syringaldazine, pH 4.0, 25°C Coriolopsis trogii
16.7
-
 substrate: guaiacol, pH 4.0, 25°C Coriolopsis trogii
23.5
-
 substrate: 2,6-dimethoxyphenol, pH 4.0, 25°C Coriolopsis trogii
36.9
-
 substrate: hydroquinone, pH 4.0, 25°C Coriolopsis trogii
39.7
-
 substrate: ferulic acid, pH 4.0, 25°C Coriolopsis trogii
50.1
-
 substrate: phloroglucinol, pH 4.0, 25°C Coriolopsis trogii
148
-
 substrate: 2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid), pH 4.0, 25°C Coriolopsis trogii

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) + O2
-
 Coriolopsis trogii ?
-
 ?
2,6-dimethoxyphenol + O2
-
 Coriolopsis trogii ?
-
 ?
catechol + O2
-
 Coriolopsis trogii ?
-
 ?
ferulic acid + O2
-
 Coriolopsis trogii ?
-
 ?
guaiacol + O2
-
 Coriolopsis trogii ?
-
 ?
hydroquinone + O2
-
 Coriolopsis trogii ?
-
 ?
additional information a high-molecular-weight kraft lignin iss effectively polymerized by the enzyme Coriolopsis trogii ?
-
 ?
phloroglucinol + O2
-
 Coriolopsis trogii ?
-
 ?
pyrogallol + O2
-
 Coriolopsis trogii ?
-
 ?
syringaldazine + O2
-
 Coriolopsis trogii ?
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 64000, SDS-PAGE Coriolopsis trogii

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
70
-
-
 Coriolopsis trogii

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
25 80 25°C: 29% of maximal activity, 80°C: about 70% of maximal activity Coriolopsis trogii

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
60
-
 half-life: 1.6 h Coriolopsis trogii

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
21.1
-
 guaiacol pH 4.0, 25°C Coriolopsis trogii
35.9
-
 syringaldazine pH 4.0, 25°C Coriolopsis trogii
50.3
-
 2,6-dimethoxyphenol pH 4.0, 25°C Coriolopsis trogii
260
-
 2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) pH 4.0, 25°C Coriolopsis trogii

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
2.2
-
 substrate: 2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) Coriolopsis trogii
3
-
 substrate: 2,6-dimethoxyphenol Coriolopsis trogii
4
-
 substrate: guaiacol Coriolopsis trogii
4.5
-
 substrate: syringaldazine Coriolopsis trogii

pH Stability

pH Stability pH Stability Maximum Comment Organism
4
-
 24 h, 25°C, about 60% loss of activity Coriolopsis trogii
6 9 24 h, 25°C, stable Coriolopsis trogii
10
-
 24 h, 25°C, about 65% loss of activity Coriolopsis trogii

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
38
-
 guaiacol pH 4.0, 25°C Coriolopsis trogii
170
-
 2,6-dimethoxyphenol pH 4.0, 25°C Coriolopsis trogii
5360
-
 syringaldazine pH 4.0, 25°C Coriolopsis trogii
35519
-
 2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) pH 4.0, 25°C Coriolopsis trogii