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Literature summary for 1.10.3.2 extracted from

  • Ademakinwa, A.; Agboola, F.
    Biochemical characterization and kinetic studies on a purified yellow laccase from newly isolated Aureobasidium pullulans NAC8 obtained from soil containing decayed plant matter (2016), J. Genet. Eng. Biotechnol., 14, 143-151 .
No PubMed abstract available
Search for more literature for 1.10.3.2

Application

Application Comment Organism
environmental protection laccase can be efficiently used to decolorize synthetic dye and help in waste water treatment Aureobasidium pullulans

Inhibitors

Inhibitors Comment Organism Structure
2-mercaptoethanol 0.05 mM, 29% loss of activity Aureobasidium pullulans
acetone 50% (v/v), 90% inhibition Aureobasidium pullulans
Dichloromethane 50% (v/v), 80% inhibition Aureobasidium pullulans
dimethyl sulfoxide 50% (v/v), 85% inhibition Aureobasidium pullulans
EDTA 0.5 mM, 29% loss of activity Aureobasidium pullulans
ethanol 50% (v/v), 80% inhibition Aureobasidium pullulans
L-cysteine 0.05 mM, 44% loss of activity Aureobasidium pullulans
methanol 50% (v/v), 70% inhibition Aureobasidium pullulans
SDS 0.05 mM, 41% loss of activity Aureobasidium pullulans
Sodium azide 0.05 mM, 50% loss of activity Aureobasidium pullulans
thioglycolic acid 2.5 mM, 50% loss of activity Aureobasidium pullulans

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.05
-
 guaiacol pH 5.0, 25°C Aureobasidium pullulans

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ 25 mM, 70% inhibition Aureobasidium pullulans
Co2+ 25 mM, 61% inhibition Aureobasidium pullulans
Mg2+ 25 mM, 35% inhibition Aureobasidium pullulans
Mn2+ 25 mM, 55% inhibition Aureobasidium pullulans
Ni2+ 25 mM, 75% inhibition Aureobasidium pullulans

Organism

Organism UniProt Comment Textmining
Aureobasidium pullulans
-
-
-
Aureobasidium pullulans NAC8
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Aureobasidium pullulans

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
caffeic acid + O2 24.3% of the activity with guaiacol Aureobasidium pullulans ?
-
 ?
caffeic acid + O2 24.3% of the activity with guaiacol Aureobasidium pullulans NAC8 ?
-
 ?
catechol + O2 85.4% of the activity with guaiacol Aureobasidium pullulans ?
-
 ?
catechol + O2 85.4% of the activity with guaiacol Aureobasidium pullulans NAC8 ?
-
 ?
gallic acid + O2 13.7% of the activity with guaiacol Aureobasidium pullulans ?
-
 ?
gallic acid + O2 13.7% of the activity with guaiacol Aureobasidium pullulans NAC8 ?
-
 ?
guaiacol + O2
-
 Aureobasidium pullulans ?
-
 ?
guaiacol + O2
-
 Aureobasidium pullulans NAC8 ?
-
 ?
tannic acid + O2 63.1 % of the activity with guaiacol Aureobasidium pullulans ?
-
 ?
tannic acid + O2 63.1 % of the activity with guaiacol Aureobasidium pullulans NAC8 ?
-
 ?

Subunits

Subunits Comment Organism
? x * 68400, SDS-PAGE Aureobasidium pullulans

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
45
-
-
 Aureobasidium pullulans

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
45 55 2 h, the enzyme retains more than 60% of its activity Aureobasidium pullulans
65 75 2 h, the enzyme loses nearly 80% of its activity Aureobasidium pullulans

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.53
-
 guaiacol pH 5.0, 25°C Aureobasidium pullulans

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5
-
 substrate: guaiacol Aureobasidium pullulans
6
-
 substrate: catechol Aureobasidium pullulans

pH Stability

pH Stability pH Stability Maximum Comment Organism
3 6 1 h, stable Aureobasidium pullulans

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
2.4
-
 guaiacol pH 5.0, 25°C Aureobasidium pullulans