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Literature summary for 1.10.3.2 extracted from

  • Safary, A.; Moniri, R.; Hamzeh-Mivehroud, M.; Dastmalchi, S.
    A strategy for soluble overexpression and biochemical characterization of halo-thermotolerant Bacillus laccase in modified E. coli (2016), J. Biotechnol., 227, 56-63 .
    View publication on PubMed
Search for more literature for 1.10.3.2

Cloned(Commentary)

Cloned (Comment) Organism
expression of a soluble and functional laccase in modified Escherichia coli, trxB2/gor2 mutant(OrigamiTMB (DE3)) at the expression level suitable for industrial application. The choice of Origami is dictated by the fact that disulfide bridges formation is necessary for properfolding of laccase Bacillus sp. (in: Bacteria)

Inhibitors

Inhibitors Comment Organism Structure
dithiothreitol 0.1 mM, complete inhibition Bacillus sp. (in: Bacteria)
L-cysteine 0.1 mM, complete inhibition Bacillus sp. (in: Bacteria)
additional information no inhibition: EDTA (10-50 mM), SDS (0.1-1 mM) Bacillus sp. (in: Bacteria)
NaCl 1000 mM, 24% inhibition Bacillus sp. (in: Bacteria)
NaN3 1 mM, 35% inhibition Bacillus sp. (in: Bacteria)

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0196
-
 syringaldazine pH 6.5, 37°C, recombinant enzyme Bacillus sp. (in: Bacteria)
0.046
-
 2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) pH 5.0, 25°C, recombinant enzyme Bacillus sp. (in: Bacteria)

Organic Solvent Stability

Organic Solvent Comment Organism
acetonitrile 30% (v/v), 70% loss of activity, recombinant enzyme Bacillus sp. (in: Bacteria)
dimethyl sulfoxide 30% (v/v), 65% loss of activity, recombinant enzyme Bacillus sp. (in: Bacteria)
Ethanol 30% (v/v), 65% loss of activity, recombinant enzyme Bacillus sp. (in: Bacteria)
Methanol 30% (v/v), 50% loss of activity, recombinant enzyme Bacillus sp. (in: Bacteria)

Organism

Organism UniProt Comment Textmining
Bacillus sp. (in: Bacteria) A0A165W3F6
-
-
Bacillus sp. (in: Bacteria) SL-1 A0A165W3F6
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant enzyme Bacillus sp. (in: Bacteria)

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
13
-
 recombinant enzyme, substrate: syringaldazine, pH 6.5, 37°C, recombinant enzyme Bacillus sp. (in: Bacteria)

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) + O2
-
 Bacillus sp. (in: Bacteria) ?
-
 ?
2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) + O2
-
 Bacillus sp. (in: Bacteria) SL-1 ?
-
 ?
syringaldazine + O2 90% of the activity compared to 2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) Bacillus sp. (in: Bacteria) ?
-
 ?
syringaldazine + O2 90% of the activity compared to 2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) Bacillus sp. (in: Bacteria) SL-1 ?
-
 ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
50
-
-
 Bacillus sp. (in: Bacteria)

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
70
-
 half-life: 60 min, recombinant enzyme Bacillus sp. (in: Bacteria)
80
-
 half-life: 20 min, recombinant enzyme Bacillus sp. (in: Bacteria)

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
23
-
 2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) pH 5.0, 25°C, recombinant enzyme Bacillus sp. (in: Bacteria)
24
-
 syringaldazine pH 6.5, 37°C, recombinant enzyme Bacillus sp. (in: Bacteria)

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
4.5
-
 substrate: 2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) Bacillus sp. (in: Bacteria)
6.5
-
 substrate: syringaldazine Bacillus sp. (in: Bacteria)

pH Stability

pH Stability pH Stability Maximum Comment Organism
3.5
-
 24 h, complete loss of activity, recombinant enzyme Bacillus sp. (in: Bacteria)
6.5
-
 72 h, 22°C, enzyme retains 21% of its activity, recombinant enzyme Bacillus sp. (in: Bacteria)
8.5
-
 72 h, 22°C, enzyme retains 17% of its activity, recombinant enzyme Bacillus sp. (in: Bacteria)