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Literature summary for 1.10.3.2 extracted from

  • Olajuyigbe, F.M.; Fatokun, C.O.
    Biochemical characterization of an extremely stable pH-versatile laccase from Sporothrix carnis CPF-05 (2017), Int. J. Biol. Macromol., 94, 535-543 .
    View publication on PubMed
Search for more literature for 1.10.3.2

Inhibitors

Inhibitors Comment Organism Structure
2-mercaptoethanol 1 mM, 23% inhibition Sporothrix carnis
Ba2+ 1 mM, 39% inhibition Sporothrix carnis
Ca2+ 1 mM, 37% inhibition Sporothrix carnis
Cu2+ 1 mM, 48% inhibition Sporothrix carnis
EDTA 1 mM, 29% inhibition Sporothrix carnis
Hg2+ 1 mM, 46% inhibition Sporothrix carnis
Mg2+ 1 mM, 45% inhibition Sporothrix carnis
Mn2+ 1 mM, 45% inhibition Sporothrix carnis
SDS 1 mM, 27% inhibition Sporothrix carnis
Sodium azide 1 mM, 27% inhibition Sporothrix carnis
Triton X-100 1 mM, 35% inhibition Sporothrix carnis
Urea 1 mM, 21% inhibition Sporothrix carnis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0316
-
 2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) pH 7.5, 30°C Sporothrix carnis

Metals/Ions

Metals/Ions Comment Organism Structure
Cu2+ 10 mM, 1.3fold activation Sporothrix carnis
Mn2+ 10 mM, 2.5fold activation Sporothrix carnis

Organic Solvent Stability

Organic Solvent Comment Organism
Acetone 50% (v/v), preincubation at 30°C for 30 min before addition of substrate, 27% loss of activity Sporothrix carnis
acetonitrile 50% (v/v), preincubation at 30°C for 30 min before addition of substrate, 39% loss of activity Sporothrix carnis
dimethyl sulfoxide 50% (v/v), preincubation at 30°C for 30 min before addition of substrate, 46% loss of activity Sporothrix carnis
dimethylformamide 50% (v/v), preincubation at 30°C for 30 min before addition of substrate, 36% loss of activity Sporothrix carnis
Ethanol 50% (v/v), preincubation at 30°C for 30 min before addition of substrate, 34% loss of activity Sporothrix carnis
Methanol 50% (v/v), preincubation at 30°C for 30 min before addition of substrate, 29% loss of activity Sporothrix carnis

Organism

Organism UniProt Comment Textmining
Sporothrix carnis
-
-
-
Sporothrix carnis CPF-05
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Sporothrix carnis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) + O2
-
 Sporothrix carnis ?
-
 ?
2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) + O2
-
 Sporothrix carnis CPF-05 ?
-
 ?
4-methylcatechol + O2 98% of the activity compared to 2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) Sporothrix carnis ?
-
 ?
4-methylcatechol + O2 98% of the activity compared to 2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) Sporothrix carnis CPF-05 ?
-
 ?
guaiacol + O2 98% of the activity compared to 2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) Sporothrix carnis ?
-
 ?
guaiacol + O2 98% of the activity compared to 2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) Sporothrix carnis CPF-05 ?
-
 ?
pyrocatechol + O2 99% of the activity compared to 2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) Sporothrix carnis ?
-
 ?
pyrocatechol + O2 99% of the activity compared to 2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) Sporothrix carnis CPF-05 ?
-
 ?
syringaldazine + O2 90% of the activity compared to 2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) Sporothrix carnis ?
-
 ?
syringaldazine + O2 90% of the activity compared to 2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) Sporothrix carnis CPF-05 ?
-
 ?

Subunits

Subunits Comment Organism
? x * 56000, SDS-PAGE Sporothrix carnis

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
50
-
 substrate: 2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) Sporothrix carnis

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
30 90 30°C: 95% of maximal activity, 90°C: 50% of maximal activity Sporothrix carnis

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
30
-
 the enzyme retains 94% of its initial activity after 180 min of incubation with 2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) as substrate Sporothrix carnis
40
-
 the enzyme retains 98% of its initial activity after 180 min of incubation with 2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) as substrate Sporothrix carnis
60
-
 the enzyme retains 91% of its initial activity after 180 min of incubation with 2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) as substrate Sporothrix carnis
70
-
 the enzyme retains 84% of its initial activity after 180 min of incubation with 2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) as substrate Sporothrix carnis
80
-
 the enzyme retains 65% of its initial activity after 180 min of incubation with 2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) as substrate Sporothrix carnis
90
-
 the enzyme retains 50% of its initial activity after 180 min of incubation with 2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) as substrate Sporothrix carnis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
 substrate: 2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) Sporothrix carnis

pH Range

pH Minimum pH Maximum Comment Organism
3 11 pH 3.0: 93% of maximal activity, pH 11.0: 91% of maximal activity Sporothrix carnis

pH Stability

pH Stability pH Stability Maximum Comment Organism
3 11 2 h, enzyme retains over 60% of its initial activity Sporothrix carnis
11
-
 2 h, no loss of activity Sporothrix carnis