Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 1.10.3.2 extracted from

  • Callejon, S.; Sendra, R.; Ferrer, S.; Pardo, I.
    Cloning and characterization of a new laccase from Lactobacillus plantarum J16 CECT 8944 catalyzing biogenic amines degradation (2016), Appl. Microbiol. Biotechnol., 100, 3113-3124 .
    View publication on PubMed
Search for more literature for 1.10.3.2

Application

Application Comment Organism
degradation eliminating toxic compounds (biogenic amines) present in fermented food and beverages Lactiplantibacillus plantarum

Cloned(Commentary)

Cloned (Comment) Organism
heterologously overexpressed in Escherichia coli Lactiplantibacillus plantarum

Inhibitors

Inhibitors Comment Organism Structure
1-ethyl-3-(3-dimethylaminopropyl) carbodiimide 99.5% inhibition Lactiplantibacillus plantarum
bipyridyl 90% inhibition Lactiplantibacillus plantarum
additional information no inhibition by 0.1 mM EDTA, laccase retains 100 % activity with 2,6-dimethoxyphenol as a substrate in the presence of clorgiline or pargyline (specific amine oxidase inhibitors) Lactiplantibacillus plantarum
Phenanthroline 95% inhibition Lactiplantibacillus plantarum
Semicarbazide 25% decrease in enzyme activity Lactiplantibacillus plantarum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.21
-
 2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) pH 7.4, 37°C Lactiplantibacillus plantarum
1.67
-
 2,6-dimethoxyphenol pH 7.4, 37°C Lactiplantibacillus plantarum

Metals/Ions

Metals/Ions Comment Organism Structure
Cu2+ multicopper enzyme Lactiplantibacillus plantarum

Organism

Organism UniProt Comment Textmining
Lactiplantibacillus plantarum
-
-
-
Lactiplantibacillus plantarum J16 CECT 8944
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Lactiplantibacillus plantarum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) + O2
-
 Lactiplantibacillus plantarum ?
-
 ?
2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) + O2
-
 Lactiplantibacillus plantarum J16 CECT 8944 ?
-
 ?
2,6-dimethoxyphenol + O2
-
 Lactiplantibacillus plantarum ?
-
 ?
2,6-dimethoxyphenol + O2
-
 Lactiplantibacillus plantarum J16 CECT 8944 ?
-
 ?
additional information 2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) acts as an effective mediator for amine oxidation by the enzyme. Higher concentrations of 2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) achieve higher tyramine degradation percentages to nearly 100% with 5 mM Lactiplantibacillus plantarum ?
-
 ?
additional information 2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) acts as an effective mediator for amine oxidation by the enzyme. Higher concentrations of 2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) achieve higher tyramine degradation percentages to nearly 100% with 5 mM Lactiplantibacillus plantarum J16 CECT 8944 ?
-
 ?

Subunits

Subunits Comment Organism
? x * 62500, SDS-PAGE Lactiplantibacillus plantarum

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
60
-
 substrate: 2,6-dimethoxyphenol Lactiplantibacillus plantarum

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
37 100 37°C: about 55% of maximal activity, 100°C: about 60% of maximal activity, substrate: 2,6-dimethoxyphenol Lactiplantibacillus plantarum

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
45 70 the enzyme is activated by 10 min of preincubation at 45-70°C, but higher temperatures promote inactivation Lactiplantibacillus plantarum
85
-
 10 min, complete inactivation Lactiplantibacillus plantarum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
3.5
-
 substrate: 2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) Lactiplantibacillus plantarum
6.5
-
 substrate: 2,6-dimethoxyphenol Lactiplantibacillus plantarum

pH Range

pH Minimum pH Maximum Comment Organism
3 4 pH 3.0: about 40% of maximal activity, pH 4.0: about 30% of maximal activity, substrate: 2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) Lactiplantibacillus plantarum
6 8 pH 6.0: about 25% of maximal activity, pH 8.0: about 70% of maximal activity, substrate: 2,6-dimethoxyphenol Lactiplantibacillus plantarum