Application | Comment | Organism |
---|---|---|
degradation | laccase can be efficiently used to decolorize synthetic dye and help in waste water treatment. Two anthraquinonic dyes (reactive blue 4 and reactive yellow brown) and two azo dyes (reactive red 11 and reactive brilliant orange) can be partially decolorized by purified laccase in the absence of a mediator. The decolorization process is efficiently promoted when methylsyringate is present, with more than 90 % of color removal occurring in 3 h at pH 7.0 or 9.0 | Bacillus subtilis X1 |
environmental protection | laccase can be efficiently used to decolorize synthetic dye and help in waste water treatment. Two anthraquinonic dyes (reactive blue 4 and reactive yellow brown) and two azo dyes (reactive red 11 and reactive brilliant orange) can be partially decolorized by purified laccase in the absence of a mediator. The decolorization process is efficiently promoted when methylsyringate is present, with more than 90 % of color removal occurring in 3 h at pH 7.0 or 9.0 | Bacillus subtilis X1 |
Cloned (Comment) | Organism |
---|---|
efficiently expressed in Escherichia coli in a biologically active form | Bacillus subtilis X1 |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus subtilis X1 | M9Y1F2 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Bacillus subtilis X1 |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) + O2 | - |
Bacillus subtilis X1 | ? | - |
? | |
2,6-dimethoxyphenol + O2 | - |
Bacillus subtilis X1 | ? | - |
? | |
additional information | two anthraquinonic dyes (reactive blue 4 and reactive yellow brown) and two azo dyes (reactive red 11 and reactive brilliant orange) can be partially decolorized by purified laccase in the absence of a mediator. The decolorization process is efficiently promoted when methylsyringate is present, with more than 90 % of color removal occurring in 3 h at pH 7.0 or 9.0 | Bacillus subtilis X1 | ? | - |
? | |
syringaldazine + O2 | - |
Bacillus subtilis X1 | ? | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
- |
Bacillus subtilis X1 |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | 80 | 30°C: about 50% of maximal activity, 80°C: about 45% of maximal activity | Bacillus subtilis X1 |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
10 h, no loss of activity | Bacillus subtilis X1 |
60 | - |
10 h, no loss of activity | Bacillus subtilis X1 |
70 | - |
half-life: 6 h | Bacillus subtilis X1 |
80 | - |
half-life: 2.5 h, 21 % of the initial activity is detected after 10 h | Bacillus subtilis X1 |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
4.4 | - |
substrate: 2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) | Bacillus subtilis X1 |
6.9 | - |
substrate: syringaldazine | Bacillus subtilis X1 |
7.3 | - |
substrate: 2,6-dimethoxyphenol | Bacillus subtilis X1 |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
3 | - |
unstable at, 23 and 15% of the original activity remains after incubation for 1 and 3 days respectively | Bacillus subtilis X1 |
7 | - |
37°C, no loss of laccase activity after 10 days of incubation | Bacillus subtilis X1 |
9 | - |
37°C, no loss of laccase activity after 10 days of incubation | Bacillus subtilis X1 |