Cloned (Comment) | Organism |
---|---|
DNA and amino acid sequence determination and analysis | Trametes sp. |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Cd2+ | - |
Trametes sp. | |
EDTA | 45% inhibition at 10 mM, 10% at 1 mM | Trametes sp. | |
Fe2+ | - |
Trametes sp. | |
L-cysteine | 90% inhibition at 10 mM, 70% at 1 mM | Trametes sp. | |
Mn2+ | - |
Trametes sp. | |
NaN3 | complete inhibition at 0.1 mM | Trametes sp. | |
SDS | 40% inhibition at 10 mM, 10% at 1 mM | Trametes sp. | |
Zn2+ | - |
Trametes sp. |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
extracellular | - |
Trametes sp. | - |
- |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | activates | Trametes sp. | |
Cu2+ | activates | Trametes sp. | |
Fe3+ | activates | Trametes sp. | |
Mg2+ | activates | Trametes sp. |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
61000 | - |
x * 61000, SDS-PAGE | Trametes sp. |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
4 catechol + O2 | Trametes sp. | - |
4 1,2-benzoquinone + 2 H2O | - |
? | |
4 catechol + O2 | Trametes sp. BS54 | - |
4 1,2-benzoquinone + 2 H2O | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Trametes sp. | - |
isolated from decayed acacia wood from Northwest of Tunisia | - |
Trametes sp. BS54 | - |
isolated from decayed acacia wood from Northwest of Tunisia | - |
Purification (Comment) | Organism |
---|---|
native extracellular laccase 10fold by ultrafilatration, anion exchange chromatography, and again ultrafiltration, to homogeneity | Trametes sp. |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
mycelium | - |
Trametes sp. | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
12.8 | - |
pH 4.0, 60°C, purified enzyme, substrate 1,2,3-trihydroxybenzene | Trametes sp. |
17 | - |
pH 4.0, 60°C, purified enzyme, substrate 3-aminobenzoic acid | Trametes sp. |
51.4 | - |
pH 4.0, 60°C, purified enzyme, substrate catechol | Trametes sp. |
61.2 | - |
pH 4.0, 60°C, purified enzyme, substrate guaiacol | Trametes sp. |
71.6 | - |
pH 4.0, 60°C, purified enzyme, substrate toluidine | Trametes sp. |
86.5 | - |
pH 4.0, 60°C, purified enzyme, substrate 2,6-dimethoxyphenol | Trametes sp. |
86.5 | - |
pH 4.0, 60°C, purified enzyme, substrate guaiacol | Trametes sp. |
112.6 | - |
pH 4.0, 60°C, purified enzyme, substrate 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) | Trametes sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4 1,2,3-trihydroxybenzene + O2 | i.e pyrogallol | Trametes sp. | ? | - |
? | |
4 1,2,3-trihydroxybenzene + O2 | i.e pyrogallol | Trametes sp. BS54 | ? | - |
? | |
4 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) + O2 | - |
Trametes sp. | ? | - |
? | |
4 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) + O2 | - |
Trametes sp. BS54 | ? | - |
? | |
4 2,6-dimethoxyphenol + O2 | - |
Trametes sp. | ? | - |
? | |
4 2,6-dimethoxyphenol + O2 | - |
Trametes sp. BS54 | ? | - |
? | |
4 3-aminobenzoic acid + O2 | - |
Trametes sp. | ? | - |
? | |
4 catechol + O2 | - |
Trametes sp. | 4 1,2-benzoquinone + 2 H2O | - |
? | |
4 catechol + O2 | - |
Trametes sp. BS54 | 4 1,2-benzoquinone + 2 H2O | - |
? | |
4 guaiacol + O2 | - |
Trametes sp. | ? | - |
? | |
4 toluidine + O2 | - |
Trametes sp. | ? | - |
? | |
additional information | substrate specificity, overview. No or poor activity with caffeic acid, hydroquinone, veratryl alcohol, or ferulic acid | Trametes sp. | ? | - |
? | |
additional information | substrate specificity, overview. No or poor activity with caffeic acid, hydroquinone, veratryl alcohol, or ferulic acid | Trametes sp. BS54 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 61000, SDS-PAGE | Trametes sp. |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
- |
Trametes sp. |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
55 | - |
loss of 50% activity after 90 min | Trametes sp. |
65 | - |
loss of 50% activity after 30 min | Trametes sp. |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
4 | - |
- |
Trametes sp. |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
4 | 9 | the purified laccase is more stable at alkaline pH of 7.0-9.0 after 24 h than at its optimum pH of 4.0 | Trametes sp. |