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Literature summary for 1.10.3.2 extracted from

  • Daassi, D.; Zouari-Mechichi, H.; Prieto, A.; Martinez, M.J.; Nasri, M.; Mechichi, T.
    Purification and biochemical characterization of a new alkali-stable laccase from Trametes sp. isolated in Tunisia: role of the enzyme in olive mill waste water treatment (2013), World J. Microbiol. Biotechnol., 29, 2145-2155.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
DNA and amino acid sequence determination and analysis Trametes sp.

Inhibitors

Inhibitors Comment Organism Structure
Cd2+
-
Trametes sp.
EDTA 45% inhibition at 10 mM, 10% at 1 mM Trametes sp.
Fe2+
-
Trametes sp.
L-cysteine 90% inhibition at 10 mM, 70% at 1 mM Trametes sp.
Mn2+
-
Trametes sp.
NaN3 complete inhibition at 0.1 mM Trametes sp.
SDS 40% inhibition at 10 mM, 10% at 1 mM Trametes sp.
Zn2+
-
Trametes sp.

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular
-
Trametes sp.
-
-

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ activates Trametes sp.
Cu2+ activates Trametes sp.
Fe3+ activates Trametes sp.
Mg2+ activates Trametes sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
61000
-
x * 61000, SDS-PAGE Trametes sp.

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4 catechol + O2 Trametes sp.
-
4 1,2-benzoquinone + 2 H2O
-
?
4 catechol + O2 Trametes sp. BS54
-
4 1,2-benzoquinone + 2 H2O
-
?

Organism

Organism UniProt Comment Textmining
Trametes sp.
-
isolated from decayed acacia wood from Northwest of Tunisia
-
Trametes sp. BS54
-
isolated from decayed acacia wood from Northwest of Tunisia
-

Purification (Commentary)

Purification (Comment) Organism
native extracellular laccase 10fold by ultrafilatration, anion exchange chromatography, and again ultrafiltration, to homogeneity Trametes sp.

Source Tissue

Source Tissue Comment Organism Textmining
mycelium
-
Trametes sp.
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
12.8
-
pH 4.0, 60°C, purified enzyme, substrate 1,2,3-trihydroxybenzene Trametes sp.
17
-
pH 4.0, 60°C, purified enzyme, substrate 3-aminobenzoic acid Trametes sp.
51.4
-
pH 4.0, 60°C, purified enzyme, substrate catechol Trametes sp.
61.2
-
pH 4.0, 60°C, purified enzyme, substrate guaiacol Trametes sp.
71.6
-
pH 4.0, 60°C, purified enzyme, substrate toluidine Trametes sp.
86.5
-
pH 4.0, 60°C, purified enzyme, substrate 2,6-dimethoxyphenol Trametes sp.
86.5
-
pH 4.0, 60°C, purified enzyme, substrate guaiacol Trametes sp.
112.6
-
pH 4.0, 60°C, purified enzyme, substrate 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) Trametes sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4 1,2,3-trihydroxybenzene + O2 i.e pyrogallol Trametes sp. ?
-
?
4 1,2,3-trihydroxybenzene + O2 i.e pyrogallol Trametes sp. BS54 ?
-
?
4 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) + O2
-
Trametes sp. ?
-
?
4 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) + O2
-
Trametes sp. BS54 ?
-
?
4 2,6-dimethoxyphenol + O2
-
Trametes sp. ?
-
?
4 2,6-dimethoxyphenol + O2
-
Trametes sp. BS54 ?
-
?
4 3-aminobenzoic acid + O2
-
Trametes sp. ?
-
?
4 catechol + O2
-
Trametes sp. 4 1,2-benzoquinone + 2 H2O
-
?
4 catechol + O2
-
Trametes sp. BS54 4 1,2-benzoquinone + 2 H2O
-
?
4 guaiacol + O2
-
Trametes sp. ?
-
?
4 toluidine + O2
-
Trametes sp. ?
-
?
additional information substrate specificity, overview. No or poor activity with caffeic acid, hydroquinone, veratryl alcohol, or ferulic acid Trametes sp. ?
-
?
additional information substrate specificity, overview. No or poor activity with caffeic acid, hydroquinone, veratryl alcohol, or ferulic acid Trametes sp. BS54 ?
-
?

Subunits

Subunits Comment Organism
? x * 61000, SDS-PAGE Trametes sp.

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
60
-
-
Trametes sp.

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
55
-
loss of 50% activity after 90 min Trametes sp.
65
-
loss of 50% activity after 30 min Trametes sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
4
-
-
Trametes sp.

pH Stability

pH Stability pH Stability Maximum Comment Organism
4 9 the purified laccase is more stable at alkaline pH of 7.0-9.0 after 24 h than at its optimum pH of 4.0 Trametes sp.