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Literature summary for 1.10.3.2 extracted from

  • Zou, Y.J.; Wang, H.X.; Ng, T.B.; Huang, C.Y.; Zhang, J.X.
    Purification and characterization of a novel laccase from the edible mushroom Hericium coralloides (2012), J. Microbiol., 50, 72-78.
    View publication on PubMed
Search for more literature for 1.10.3.2

Inhibitors

Inhibitors Comment Organism Structure
Ca2+ inhibits the enzyme activity at 100 mM but increases it at 12.5-50 mM Hericium coralloides
Cd2+ inhibits the enzyme activity at 100 mM but increases it at 12.5-50 mM Hericium coralloides
Co2+ inhibits the enzyme activity at 100 mM but increases it at 12.5-50 mM Hericium coralloides
Fe2+
-
 Hericium coralloides
Hg2+
-
 Hericium coralloides
K+ inhibits the enzyme activity at 100 mM but increases it at 12.5-50 mM Hericium coralloides
Mn2+ inhibits the enzyme activity at 100 mM but increases it at 12.5-50 mM Hericium coralloides
Zn2+ 22% inhibition at 100 mM Hericium coralloides

Metals/Ions

Metals/Ions Comment Organism Structure
Al3+ activates Hericium coralloides
Ca2+ inhibits the enzyme activity at 100 mM but increases it at 12.5-50 mM Hericium coralloides
Cd2+ inhibits the enzyme activity at 100 mM but increases it at 12.5-50 mM Hericium coralloides
Co2+ inhibits the enzyme activity at 100 mM but increases it at 12.5-50 mM Hericium coralloides
K+ inhibits the enzyme activity at 100 mM but increases it at 12.5-50 mM Hericium coralloides
Mg2+ activates Hericium coralloides
Mn2+ inhibits the enzyme activity at 100 mM but increases it at 12.5-50 mM Hericium coralloides

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
65000
-
 native enzyme, gel filtration Hericium coralloides

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4 catechol + O2 Hericium coralloides
-
 4 1,2-benzoquinone + 2 H2O
-
 ?

Organism

Organism UniProt Comment Textmining
Hericium coralloides
-
-
-

Purification (Commentary)

Purification (Comment) Organism
native enzyme 24.3fold by two different steps of anion exchange chromatography, and gel filtration Hericium coralloides

Source Tissue

Source Tissue Comment Organism Textmining
fruit body dried Hericium coralloides
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) + O2 best substrate Hericium coralloides ?
-
 ?
4 2-methoxyphenol + O2
-
 Hericium coralloides ?
-
 ?
4 catechol + O2
-
 Hericium coralloides 4 1,2-benzoquinone + 2 H2O
-
 ?
4 N,N-dimethyl-1,4-phenylenediamine + O2
-
 Hericium coralloides ?
-
 ?
4 pyrogallol + O2
-
 Hericium coralloides ?
-
 ?

Subunits

Subunits Comment Organism
monomer x * 65000, SDS-PAGE Hericium coralloides

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
40
-
-
 Hericium coralloides

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
20 100 24% of maximal activity at 100°C, 65% at 20°C, activity profile, overview Hericium coralloides

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
100
-
 purified native enzyme, 10 min, inactivation Hericium coralloides

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
2.2
-
-
 Hericium coralloides

General Information

General Information Comment Organism
additional information the laccase does not exert any antiproliferative activity against Hep-G2 or MCF-7 tumor cell lines at a concentration of 0.06 mM, but shows significant activity toward human immunodeficiency virus-1 reverse transcriptase with an IC50 of 60 nM Hericium coralloides