Application | Comment | Organism |
---|---|---|
synthesis | owing to their broad substrate range laccases are considered to be versatile biocatalysts which are capable of oxidizing natural and non-natural industrial compounds, with water as sole by-product | Bacillus pumilus |
Cloned (Comment) | Organism |
---|---|
CotA-type laccase, DNA and amino acid sequence determination and analysis, recombinant expression in Escherichia coli strain JM109 | Bacillus pumilus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | kinetics do not fit a Michaelis-Menten equation | Bacillus pumilus | |
0.08 | - |
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) | pH 4.0, 22°C, recombinant enzyme | Bacillus pumilus | |
0.68 | - |
2,6-dimethoxyphenol | pH 7.0, 22°C, recombinant enzyme | Bacillus pumilus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Cu2+ | the CotA-type laccase is associated with 4 Cu2+ ions, content determined by inductive coupled plasma optical emission spectroscopy | Bacillus pumilus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
58000 | - |
x * 58000, recombinant CotA-type laccase, SDS-PAGE | Bacillus pumilus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus pumilus | - |
gene cotA | - |
Bacillus pumilus DSM 27 | - |
gene cotA | - |
Purification (Comment) | Organism |
---|---|
recombinant CotA-type laccase from Escherichia coli strain JM109 by anion exchange chromatography, ultrafiltration, and gel filtration | Bacillus pumilus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(2E)-3-(3,4-dihydroxyphenyl)prop-2-enoic acid + O2 | - |
Bacillus pumilus | ? | - |
? | |
(2E)-3-(4-hydroxy-3-methoxyphenyl)prop-2-enoic acid + O2 | - |
Bacillus pumilus | ? | - |
? | |
(2Z,2'Z)-2,2'-(1Z,2Z)-hydrazine-1,2-diylidenebis(3-ethyl-2,3-dihydro-1,3-benzothiazole-6-sulfonic acid) + O2 | - |
Bacillus pumilus | ? | - |
? | |
1-(2,4,5-trihydroxyphenyl)butan-1-one + O2 | - |
Bacillus pumilus | ? | - |
? | |
1-(4-hydroxy-3,5-dimethoxyphenyl)ethanone + O2 | - |
Bacillus pumilus | ? | - |
? | |
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) + O2 | - |
Bacillus pumilus | ? | - |
? | |
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) + O2 | - |
Bacillus pumilus DSM 27 | ? | - |
? | |
2,6-dimethoxyphenol + O2 | - |
Bacillus pumilus | ? | - |
? | |
2,6-dimethoxyphenol + O2 | - |
Bacillus pumilus DSM 27 | ? | - |
? | |
3,4,5-trihydroxybenzoic acid + O2 | - |
Bacillus pumilus | ? | - |
? | |
3,4,5-trihydroxybenzoic acid + O2 | - |
Bacillus pumilus DSM 27 | ? | - |
? | |
3,4-dihydroxybenzoic acid + O2 | - |
Bacillus pumilus | ? | - |
? | |
3,4-dihydroxybenzoic acid + O2 | - |
Bacillus pumilus DSM 27 | ? | - |
? | |
3-amino-4-hydroxybenzoic acid + O2 | - |
Bacillus pumilus | ? | - |
? | |
3-hydroxy-L-tyrosine + O2 | - |
Bacillus pumilus | ? | - |
? | |
4-(2-aminoethyl)benzene-1,2-diol + O2 | - |
Bacillus pumilus | ? | - |
? | |
4-(hydroxymethyl)-2-methoxyphenol + O2 | - |
Bacillus pumilus | ? | - |
? | |
4-amino-3-hydroxybenzoic acid + O2 | - |
Bacillus pumilus | ? | - |
? | |
4-hydroxy-3,5-dimethoxybenzaldehyde + O2 | - |
Bacillus pumilus | ? | - |
? | |
4-hydroxy-3,5-dimethoxybenzoic acid + O2 | - |
Bacillus pumilus | ? | - |
? | |
4-methylbenzene-1,2-diol + O2 | - |
Bacillus pumilus | ? | - |
? | |
5-(hydroxymethyl)-2-methoxyphenol + O2 | - |
Bacillus pumilus | ? | - |
? | |
benzene-1,2,3-triol + O2 | - |
Bacillus pumilus | ? | - |
? | |
benzene-1,2-diol + O2 | - |
Bacillus pumilus | ? | - |
? | |
syringaldazine + O2 | - |
Bacillus pumilus | ? | - |
? | |
syringaldazine + O2 | - |
Bacillus pumilus DSM 27 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 58000, recombinant CotA-type laccase, SDS-PAGE | Bacillus pumilus |
Synonyms | Comment | Organism |
---|---|---|
CotA-type laccase | - |
Bacillus pumilus |
spore coat protein A | - |
Bacillus pumilus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
70 | - |
- |
Bacillus pumilus |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
20 | 75 | activity range | Bacillus pumilus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
4.9 | - |
syringaldazine | pH 6.5, 22°C, recombinant enzyme, with 0.8% v/v DMSO | Bacillus pumilus | |
11 | - |
2,6-dimethoxyphenol | pH 7.0, 22°C, recombinant enzyme | Bacillus pumilus | |
66 | - |
syringaldazine | pH 7.0, 22°C, recombinant enzyme, with 25% v/v DMSO | Bacillus pumilus | |
291 | - |
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) | pH 4.0, 22°C, recombinant enzyme | Bacillus pumilus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
4 | 7 | broad optimum, dependent on the substrate | Bacillus pumilus |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
2 | 8 | activity range | Bacillus pumilus |