Cloned (Comment) | Organism |
---|---|
enzyme expression in Trichoderma reesei under the strong cbh1 (cel7A) promoter in a strain in which the major cellulase gene cbh1 is disrupted | Melanocarpus albomyces |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | Michaelis-Menten steady-state kinetics, overview | Trametes versicolor | |
additional information | - |
additional information | Michaelis-Menten steady-state kinetics, overview | Melanocarpus albomyces | |
additional information | - |
additional information | Michaelis-Menten steady-state kinetics, overview | Trametes hirsuta | |
additional information | - |
additional information | Michaelis-Menten steady-state kinetics, overview | Toxicodendron vernicifluum | |
0.0024 | - |
4-hydroxy-3,5-dimethoxybenzaldehyde azine | pH 5.0, 25°C | Melanocarpus albomyces | |
0.006 | - |
dopamine | pH 5.0, 25°C | Melanocarpus albomyces | |
0.031 | - |
catechol | pH 5.0, 25°C | Melanocarpus albomyces | |
0.034 | - |
4-hydroxy-3,5-dimethoxybenzaldehyde azine | pH 5.0, 25°C | Trametes hirsuta | |
0.041 | - |
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) | pH 5.0, 25°C | Trametes hirsuta | |
0.045 | - |
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) | pH 5.0, 25°C | Toxicodendron vernicifluum | |
0.066 | - |
4-hydroxy-3,5-dimethoxybenzaldehyde azine | pH 5.0, 25°C | Trametes versicolor | |
0.069 | - |
4-hydroxy-3,5-dimethoxybenzaldehyde azine | pH 5.0, 25°C | Toxicodendron vernicifluum | |
0.092 | - |
ferrocenemonocarboxylic acid | pH 5.0, 25°C | Trametes versicolor | |
0.098 | - |
catechol | pH 5.0, 25°C | Toxicodendron vernicifluum | |
0.13 | - |
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) | pH 5.0, 25°C | Trametes versicolor | |
0.16 | - |
catechol | pH 5.0, 25°C | Trametes hirsuta | |
0.17 | - |
dopamine | pH 5.0, 25°C | Toxicodendron vernicifluum | |
0.28 | - |
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) | pH 5.0, 25°C | Melanocarpus albomyces | |
0.29 | - |
dopamine | pH 5.0, 25°C | Trametes hirsuta | |
0.32 | - |
ferrocyanide | pH 5.0, 25°C | Trametes hirsuta | |
0.38 | - |
ferrocenemonocarboxylic acid | pH 5.0, 25°C | Toxicodendron vernicifluum | |
0.41 | - |
ferrocenemonocarboxylic acid | pH 5.0, 25°C | Melanocarpus albomyces | |
0.45 | - |
ferrocenemonocarboxylic acid | pH 5.0, 25°C | Trametes hirsuta | |
0.79 | - |
ferrocyanide | pH 5.0, 25°C | Toxicodendron vernicifluum | |
0.83 | - |
ferrocyanide | pH 5.0, 25°C | Trametes versicolor | |
0.89 | - |
ferrocyanide | pH 5.0, 25°C | Melanocarpus albomyces | |
1.11 | - |
catechol | pH 5.0, 25°C | Trametes versicolor | |
2.1 | - |
dopamine | pH 5.0, 25°C | Trametes versicolor |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
55000 | - |
x * 55000, native enzyme, SDS-PAGE | Trametes hirsuta |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
4 catechol + O2 | Trametes versicolor | - |
4 1,2-benzoquinone + 2 H2O | - |
? | |
4 catechol + O2 | Melanocarpus albomyces | - |
4 1,2-benzoquinone + 2 H2O | - |
? | |
4 catechol + O2 | Trametes hirsuta | - |
4 1,2-benzoquinone + 2 H2O | - |
? | |
4 catechol + O2 | Toxicodendron vernicifluum | - |
4 1,2-benzoquinone + 2 H2O | - |
? | |
4 catechol + O2 | Trametes hirsuta VTT D-95443 | - |
4 1,2-benzoquinone + 2 H2O | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Melanocarpus albomyces | - |
- |
- |
Toxicodendron vernicifluum | Q8H979 | - |
- |
Trametes hirsuta | Q02497 | - |
- |
Trametes hirsuta VTT D-95443 | Q02497 | - |
- |
Trametes versicolor | - |
- |
- |
Purification (Comment) | Organism |
---|---|
native enzyme from culture filtrate by anion exchange and hydrophobic interaction chromatography | Trametes hirsuta |
recombinant enzyme from a Trichoderma reeseicbh1 cbh1 disruption mutant strain | Melanocarpus albomyces |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
commercial preparation | - |
Trametes versicolor | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(2,2,6,6-tetramethyl-piperidine-N-oxyl) radical + O2 | - |
Trametes versicolor | ? | - |
? | |
(2,2,6,6-tetramethyl-piperidine-N-oxyl) radical + O2 | - |
Melanocarpus albomyces | ? | - |
? | |
(2,2,6,6-tetramethyl-piperidine-N-oxyl) radical + O2 | - |
Trametes hirsuta | ? | - |
? | |
(2,2,6,6-tetramethyl-piperidine-N-oxyl) radical + O2 | - |
Toxicodendron vernicifluum | ? | - |
? | |
(2,2,6,6-tetramethyl-piperidine-N-oxyl) radical + O2 | - |
Trametes hirsuta VTT D-95443 | ? | - |
? | |
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) + O2 | - |
Trametes versicolor | ? | - |
? | |
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) + O2 | - |
Melanocarpus albomyces | ? | - |
? | |
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) + O2 | - |
Trametes hirsuta | ? | - |
? | |
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) + O2 | - |
Toxicodendron vernicifluum | ? | - |
? | |
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) + O2 | - |
Trametes hirsuta VTT D-95443 | ? | - |
? | |
4 catechol + O2 | - |
Trametes versicolor | 4 1,2-benzoquinone + 2 H2O | - |
? | |
4 catechol + O2 | - |
Melanocarpus albomyces | 4 1,2-benzoquinone + 2 H2O | - |
? | |
4 catechol + O2 | - |
Trametes hirsuta | 4 1,2-benzoquinone + 2 H2O | - |
? | |
4 catechol + O2 | - |
Toxicodendron vernicifluum | 4 1,2-benzoquinone + 2 H2O | - |
? | |
4 catechol + O2 | - |
Trametes hirsuta VTT D-95443 | 4 1,2-benzoquinone + 2 H2O | - |
? | |
4-hydroxy-3,5-dimethoxybenzaldehyde azine + O2 | i.e. syringaldazine | Trametes versicolor | ? | - |
? | |
4-hydroxy-3,5-dimethoxybenzaldehyde azine + O2 | i.e. syringaldazine | Melanocarpus albomyces | ? | - |
? | |
4-hydroxy-3,5-dimethoxybenzaldehyde azine + O2 | i.e. syringaldazine | Trametes hirsuta | ? | - |
? | |
4-hydroxy-3,5-dimethoxybenzaldehyde azine + O2 | i.e. syringaldazine | Toxicodendron vernicifluum | ? | - |
? | |
dopamine + O2 | - |
Trametes versicolor | ? | - |
? | |
dopamine + O2 | - |
Melanocarpus albomyces | ? | - |
? | |
dopamine + O2 | - |
Trametes hirsuta | ? | - |
? | |
dopamine + O2 | - |
Toxicodendron vernicifluum | ? | - |
? | |
dopamine + O2 | - |
Trametes hirsuta VTT D-95443 | ? | - |
? | |
ferrocenemonocarboxylic acid + O2 | - |
Trametes versicolor | ? | - |
? | |
ferrocenemonocarboxylic acid + O2 | - |
Melanocarpus albomyces | ? | - |
? | |
ferrocenemonocarboxylic acid + O2 | - |
Trametes hirsuta | ? | - |
? | |
ferrocenemonocarboxylic acid + O2 | - |
Toxicodendron vernicifluum | ? | - |
? | |
ferrocyanide + O2 | - |
Trametes versicolor | ? | - |
? | |
ferrocyanide + O2 | - |
Melanocarpus albomyces | ? | - |
? | |
ferrocyanide + O2 | - |
Trametes hirsuta | ? | - |
? | |
ferrocyanide + O2 | - |
Toxicodendron vernicifluum | ? | - |
? | |
ferrocyanide + O2 | - |
Trametes hirsuta VTT D-95443 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 55000, native enzyme, SDS-PAGE | Trametes hirsuta |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at | Trametes versicolor |
25 | - |
assay at | Melanocarpus albomyces |
25 | - |
assay at | Trametes hirsuta |
25 | - |
assay at | Toxicodendron vernicifluum |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.1 | - |
ferrocyanide | pH 5.0, 25°C | Toxicodendron vernicifluum | |
0.32 | - |
4-hydroxy-3,5-dimethoxybenzaldehyde azine | pH 5.0, 25°C | Toxicodendron vernicifluum | |
0.92 | - |
ferrocenemonocarboxylic acid | pH 5.0, 25°C | Toxicodendron vernicifluum | |
2.6 | - |
catechol | pH 5.0, 25°C | Toxicodendron vernicifluum | |
4.4 | - |
dopamine | pH 5.0, 25°C | Toxicodendron vernicifluum | |
10.1 | - |
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) | pH 5.0, 25°C | Toxicodendron vernicifluum | |
64 | - |
ferrocyanide | pH 5.0, 25°C | Melanocarpus albomyces | |
75 | - |
ferrocenemonocarboxylic acid | pH 5.0, 25°C | Melanocarpus albomyces | |
77 | - |
4-hydroxy-3,5-dimethoxybenzaldehyde azine | pH 5.0, 25°C | Trametes versicolor | |
83 | - |
ferrocenemonocarboxylic acid | pH 5.0, 25°C | Trametes versicolor | |
88 | - |
catechol | pH 5.0, 25°C | Melanocarpus albomyces | |
108 | - |
ferrocyanide | pH 5.0, 25°C | Trametes hirsuta | |
109 | - |
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) | pH 5.0, 25°C | Melanocarpus albomyces | |
115 | - |
catechol | pH 5.0, 25°C | Trametes versicolor | |
116 | - |
dopamine | pH 5.0, 25°C | Melanocarpus albomyces | |
122 | - |
4-hydroxy-3,5-dimethoxybenzaldehyde azine | pH 5.0, 25°C | Trametes hirsuta | |
130 | - |
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) | pH 5.0, 25°C | Trametes versicolor | |
137 | - |
ferrocenemonocarboxylic acid | pH 5.0, 25°C | Trametes hirsuta | |
140 | - |
4-hydroxy-3,5-dimethoxybenzaldehyde azine | pH 5.0, 25°C | Melanocarpus albomyces | |
184 | - |
dopamine | pH 5.0, 25°C | Trametes hirsuta | |
186 | - |
dopamine | pH 5.0, 25°C | Trametes versicolor | |
196 | - |
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) | pH 5.0, 25°C | Trametes hirsuta | |
202 | - |
catechol | pH 5.0, 25°C | Trametes hirsuta | |
215 | - |
ferrocyanide | pH 5.0, 25°C | Trametes versicolor |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
4.5 | 5 | assay at | Trametes versicolor |
4.5 | 5 | assay at | Melanocarpus albomyces |
4.5 | 5 | assay at | Trametes hirsuta |
4.5 | 5 | assay at | Toxicodendron vernicifluum |
General Information | Comment | Organism |
---|---|---|
additional information | the laccase from Melanocarpus albomyces is a low redox potential enzyme. Structure-function study, mass spectrometry, overview | Melanocarpus albomyces |
additional information | the laccase from Rhus vernicifera is a low redox potential enzyme. Structure-function study, mass spectrometry, overview | Toxicodendron vernicifluum |
additional information | the laccase from Trametes hirsuta is a high redox potential enzyme. Structure-function study, mass spectrometry, overview | Trametes hirsuta |
additional information | the laccase from Trametes versicolor is a high redox potential enzyme. Structure-function study, mass spectrometry, overview | Trametes versicolor |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.13 | - |
ferrocyanide | pH 5.0, 25°C | Toxicodendron vernicifluum | |
2.3 | - |
ferrocenemonocarboxylic acid | pH 5.0, 25°C | Toxicodendron vernicifluum | |
4.3 | - |
4-hydroxy-3,5-dimethoxybenzaldehyde azine | pH 5.0, 25°C | Toxicodendron vernicifluum | |
25.1 | - |
dopamine | pH 5.0, 25°C | Toxicodendron vernicifluum | |
26.5 | - |
catechol | pH 5.0, 25°C | Toxicodendron vernicifluum | |
72 | - |
ferrocyanide | pH 5.0, 25°C | Melanocarpus albomyces | |
88 | - |
dopamine | pH 5.0, 25°C | Trametes versicolor | |
103 | - |
catechol | pH 5.0, 25°C | Trametes versicolor | |
185 | - |
ferrocenemonocarboxylic acid | pH 5.0, 25°C | Melanocarpus albomyces | |
222 | - |
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) | pH 5.0, 25°C | Toxicodendron vernicifluum | |
259 | - |
ferrocyanide | pH 5.0, 25°C | Trametes versicolor | |
306 | - |
ferrocenemonocarboxylic acid | pH 5.0, 25°C | Trametes hirsuta | |
334 | - |
ferrocyanide | pH 5.0, 25°C | Trametes hirsuta | |
380 | - |
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) | pH 5.0, 25°C | Melanocarpus albomyces | |
643 | - |
dopamine | pH 5.0, 25°C | Trametes hirsuta | |
956 | - |
ferrocenemonocarboxylic acid | pH 5.0, 25°C | Trametes versicolor | |
1024 | - |
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) | pH 5.0, 25°C | Trametes versicolor | |
1167 | - |
4-hydroxy-3,5-dimethoxybenzaldehyde azine | pH 5.0, 25°C | Trametes versicolor | |
1208 | - |
dopamine | pH 5.0, 25°C | Melanocarpus albomyces | |
1232 | - |
catechol | pH 5.0, 25°C | Trametes hirsuta | |
2838 | - |
catechol | pH 5.0, 25°C | Melanocarpus albomyces | |
3288 | - |
4-hydroxy-3,5-dimethoxybenzaldehyde azine | pH 5.0, 25°C | Trametes hirsuta | |
4780 | - |
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) | pH 5.0, 25°C | Trametes hirsuta | |
58330 | - |
4-hydroxy-3,5-dimethoxybenzaldehyde azine | pH 5.0, 25°C | Melanocarpus albomyces |