Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Al3+ | weak inhibition | Leucocalocybe mongolica | |
Fe2+ | weak inhibition | Leucocalocybe mongolica | |
Hg2+ | strong inhibition | Leucocalocybe mongolica | |
Pb2+ | weak inhibition | Leucocalocybe mongolica |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0023 | - |
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) | in 67 mM sodium acetate buffer (pH 4.5), at 30°C | Leucocalocybe mongolica |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Cu2+ | the enzyme is strongly stimulated by Cu2+ | Leucocalocybe mongolica |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
66000 | - |
1 * 66000, SDS-PAGE | Leucocalocybe mongolica |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Leucocalocybe mongolica | - |
- |
- |
Purification (Comment) | Organism |
---|---|
DEAE-cellulose column chromatography, CM-cellulose column chromatography, Q-Sepharose column chromatography, and Superdex 75 gel filtration | Leucocalocybe mongolica |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
the specific activity of the crude enzyme in 67 mM sodium acetate buffer (pH 4.5), at 30°C, using 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) as substrate is 11.3 units/mg, the specific activity of the 131fold purification enzyme in 67 mM sodium acetate buffer (pH 4.5), at 30°C, using 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) as substrate is 1480 units/mg. One unit of enzyme activity is defined as the amount of enzyme required to produce an absorbance increase at 420 nm of 1 per minute per ml of reaction mixture | Leucocalocybe mongolica |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) + O2 | 100% activity | Leucocalocybe mongolica | ? | - |
? | |
2-methoxyphenol + O2 | 8.6% activity compared to 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) | Leucocalocybe mongolica | ? | - |
? | |
catechol + O2 | 11.5% activity compared to 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) | Leucocalocybe mongolica | ? | - |
? | |
hydroquinone + O2 | 13.5% activity compared to 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) | Leucocalocybe mongolica | ? | - |
? | |
additional information | no activity with L-tyrosine | Leucocalocybe mongolica | ? | - |
? | |
N,N-dimethyl-1,4-phenylenediamine + O2 | 49.4% activity compared to 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) | Leucocalocybe mongolica | ? | - |
? | |
pyrogallol + O2 | 3.5% activity compared to 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) | Leucocalocybe mongolica | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 66000, SDS-PAGE | Leucocalocybe mongolica |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
purified enzyme | Leucocalocybe mongolica |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
5 | 70 | the enzyme activity increases by about 70% to its maximum at 30°C when the temperature is elevated from 5°C, and then falls to about 10% of the maximal value when the temperature is raised to 70°C | Leucocalocybe mongolica |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1480 | - |
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) | in 67 mM sodium acetate buffer (pH 4.5), at 30°C | Leucocalocybe mongolica |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
2 | 3 | purified enzyme | Leucocalocybe mongolica |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
2 | 8 | the activity rises abruptly when the pH is increased from slightly below 2.0. The activity declines sharply when the pH is raised from 4.0 to 5.0 and is indiscernible at pH 6.0-8.0 | Leucocalocybe mongolica |
General Information | Comment | Organism |
---|---|---|
physiological function | laccase inhibits HIV-1 reverse transcriptase and proliferation of hepatoma Hep-G2 cells and breast cancer MCF-7 cells with an IC50 of 0.00065 mM, 0.0014 mM, and 0.0042 mM, respectively, indicating that it is also an antipathogenic protein | Leucocalocybe mongolica |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
643500 | - |
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) | in 67 mM sodium acetate buffer (pH 4.5), at 30°C | Leucocalocybe mongolica |