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Literature summary for 1.10.3.2 extracted from

  • Nakade, K.; Nakagawa, Y.; Yano, A.; Sato, T.; Sakamoto, Y.
    Characterization of an extracellular laccase, PbLac1, purified from Polyporus brumalis (2010), Fungal Biol., 114, 609-618.
    View publication on PubMed
Search for more literature for 1.10.3.2

Cloned(Commentary)

Cloned (Comment) Organism
-
 Polyporus brumalis

Inhibitors

Inhibitors Comment Organism Structure
4-coumaric acid 32.5% residual activity at 1 mM Polyporus brumalis
Ca2+ 89.8% residual activity at 1 mM Polyporus brumalis
Cd2+ 88.0% residual activity at 1 mM Polyporus brumalis
Co2+ 75.3% residual activity at 01 mM Polyporus brumalis
dithiothreitol completely inhibited by 1.0 mM dithiothreitol Polyporus brumalis
EDTA 50% residual activity at 10 mM Polyporus brumalis
K+ 65.1% residual activity at 10 mM Polyporus brumalis
kojic acid 27.7% residual activity at 1 mM Polyporus brumalis
L-cysteine completely inhibited by 1.0 mM L-cysteine Polyporus brumalis
Mg2+ 68.8% residual activity at 10 mM Polyporus brumalis
Mn2+ 82.3% residual activity at 10 mM Polyporus brumalis
Na+ 56.6% residual activity at 10 mM Polyporus brumalis
NaN3 8.9% residual activity at 1 mM Polyporus brumalis
Sn2+ 4.6% residual activity at 1 mM Polyporus brumalis
tropolone 9.7% residual activity at 1 mM Polyporus brumalis
Zn2+ 65.7% residual activity at 1 mM Polyporus brumalis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.02
-
 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) in McIlvaine buffer (pH 4.0), at 30°C Polyporus brumalis
0.07
-
 2,6-dimethoxyphenol in McIlvaine buffer (pH 4.0), at 30°C Polyporus brumalis
0.12
-
 ferulic acid in McIlvaine buffer (pH 5.0), at 30°C Polyporus brumalis
0.27
-
 4-phenylenediamine in McIlvaine buffer (pH 5.0), at 30°C Polyporus brumalis
0.56
-
 pyrogallol in McIlvaine buffer (pH 4.0), at 30°C Polyporus brumalis
0.66
-
 3,4-dihydroxy L-phenylalanine in McIlvaine buffer (pH 4.0), at 30°C Polyporus brumalis
8.06
-
 catechol in McIlvaine buffer (pH 4.0), at 30°C Polyporus brumalis
10.7
-
 guaiacol in McIlvaine buffer (pH 4.0), at 30°C Polyporus brumalis

Metals/Ions

Metals/Ions Comment Organism Structure
Cu2+ 138% activity at 10 mM Polyporus brumalis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
63400
-
 estimated from SDS-PAGE Polyporus brumalis

Organism

Organism UniProt Comment Textmining
Polyporus brumalis A3F8Z8
-
-
Polyporus brumalis ibrc05015 A3F8Z8
-
-

Purification (Commentary)

Purification (Comment) Organism
ammonium sulfate precipitation and Sepharose Q column chromatography Polyporus brumalis

Source Tissue

Source Tissue Comment Organism Textmining
mycelium
-
 Polyporus brumalis
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) + O2
-
 Polyporus brumalis ?
-
 ?
2,6-dimethoxyphenol + O2
-
 Polyporus brumalis ?
-
 ?
3,4-dihydroxy L-phenylalanine + O2
-
 Polyporus brumalis ?
-
 ?
4-phenylenediamine + O2
-
 Polyporus brumalis ?
-
 ?
catechol + O2
-
 Polyporus brumalis ?
-
 ?
catechol + O2
-
 Polyporus brumalis ibrc05015 ?
-
 ?
ferulic acid + O2
-
 Polyporus brumalis ?
-
 ?
ferulic acid + O2
-
 Polyporus brumalis ibrc05015 ?
-
 ?
guaiacol + O2
-
 Polyporus brumalis ?
-
 ?
additional information Lac1 decolorizes 0.02% (v/v) Remazol Brilliant Blue R and Bromophenol Blue more than 90% in 100 min. Lac1 completely decolorizes 0.002% (v/v) in 100 min. Reactive Orange 16 and Poly R-478 are not decolorized by Lac1 alone. In addition, Lac1 decolorized a wide range of substrates, such as the carcinogen, Poly R-478, in the presence of violuric acid as mediator Polyporus brumalis ?
-
 ?
additional information Lac1 does not oxidize L-tyrosine Polyporus brumalis ?
-
 ?
additional information Lac1 decolorizes 0.02% (v/v) Remazol Brilliant Blue R and Bromophenol Blue more than 90% in 100 min. Lac1 completely decolorizes 0.002% (v/v) in 100 min. Reactive Orange 16 and Poly R-478 are not decolorized by Lac1 alone. In addition, Lac1 decolorized a wide range of substrates, such as the carcinogen, Poly R-478, in the presence of violuric acid as mediator Polyporus brumalis ibrc05015 ?
-
 ?
additional information Lac1 does not oxidize L-tyrosine Polyporus brumalis ibrc05015 ?
-
 ?
pyrogallol + O2
-
 Polyporus brumalis ?
-
 ?
pyrogallol + O2
-
 Polyporus brumalis ibrc05015 ?
-
 ?

Synonyms

Synonyms Comment Organism
LAC1
-
 Polyporus brumalis
p-benzenediol:dioxygen oxidoreductase
-
 Polyporus brumalis

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
40
-
 at pH. 4.0 Polyporus brumalis

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
20 60
-
 Polyporus brumalis

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
50 80 the Lac activity of Lac1 remains 80% after incubation at 50°C for 30 min. However, incubated at 60°C, the remaining activity declines in less than 80%. The enzyme is inactive at 70-80°C Polyporus brumalis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
4
-
-
 Polyporus brumalis

pH Range

pH Minimum pH Maximum Comment Organism
3 6
-
 Polyporus brumalis