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Literature summary for 1.10.3.2 extracted from

  • Pakhadnia, Y.G.; Malinouski, N.I.; Lapko, A.G.
    Purification and characteristics of an enzyme with both bilirubin oxidase and laccase activities from mycelium of the basidiomycete Pleurotus ostreatus (2009), Biochemistry (Moscow), 74, 1027-1034.
    View publication on PubMed
Search for more literature for 1.10.3.2

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.02
-
 syringaldazine isoform POXC, pH 6.0, 25°C Pleurotus ostreatus
0.036
-
 syringaldazine isoform POXA3a, pH 6.0, 25°C Pleurotus ostreatus
0.07
-
 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) isoform POXA3a, pH 3.6, 25°C Pleurotus ostreatus
0.074
-
 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) isoform POXA3b, pH 3.6, 25°C Pleurotus ostreatus
0.079
-
 syringaldazine isoform POXA3b, pH 6.0, 25°C Pleurotus ostreatus
0.09
-
 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) isoform POXA1w, pH 3.0, 25°C Pleurotus ostreatus
0.12
-
 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) isoform POXA2, pH 3.0, 25°C Pleurotus ostreatus
0.13
-
 syringaldazine isoform POXA1w, pH 6.0, 25°C Pleurotus ostreatus
0.14
-
 syringaldazine isoform POXA2, pH 6.0, 25°C Pleurotus ostreatus
0.22
-
 syringaldazine isoform POXA1b, pH 6.0, 25°C Pleurotus ostreatus
0.23
-
 2,6-dimethoxyphenol isoform POXC, pH 3.0-5.0, 25°C Pleurotus ostreatus
0.26
-
 2,6-dimethoxyphenol isoform POXA1b, pH 4.0-5.0, 25°C Pleurotus ostreatus
0.28
-
 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) isoform POXC, pH 3.0, 25°C Pleurotus ostreatus
0.37
-
 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) isoform POXA1b, pH 3.0, 25°C Pleurotus ostreatus
0.74
-
 2,6-dimethoxyphenol isoform POXA2, pH 6.5, 25°C Pleurotus ostreatus
1.2
-
 guaiacol isoform POXC, pH 6.0, 25°C Pleurotus ostreatus
2.1
-
 2,6-dimethoxyphenol isoform POXA1w, pH 3.0-5.0, 25°C Pleurotus ostreatus
3.1
-
 guaiacol isoform POXA2, pH 6.0, 25°C Pleurotus ostreatus
8.8
-
 2,6-dimethoxyphenol isoform POXA3b, pH 5.5, 25°C Pleurotus ostreatus
14
-
 2,6-dimethoxyphenol isoform POXA3a, pH 5.5, 25°C Pleurotus ostreatus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
59000
-
 1 * 59000, isoform POXC, SDS-PAGE Pleurotus ostreatus
61000
-
 1 * 61000, isoform POXA1w, SDS-PAGE Pleurotus ostreatus
62000
-
 1 * 62000, isoform POXA1b, SDS-PAGE Pleurotus ostreatus
67000
-
 1 * 67000, isoform POXA2, SDS-PAGE Pleurotus ostreatus

Organism

Organism UniProt Comment Textmining
Pleurotus ostreatus
-
-
-
Pleurotus ostreatus 43 BIM F-306D
-
-
-

Purification (Commentary)

Purification (Comment) Organism
DEAE-cellulose column chromatography, ConA-Sepharose column chromatography, and Toyopearl HW-55 column chromatography Pleurotus ostreatus

Source Tissue

Source Tissue Comment Organism Textmining
mycelium
-
 Pleurotus ostreatus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
19.1
-
 crude enzyme, using 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) as substrate, at pH 3.0 and 50°C Pleurotus ostreatus
261
-
 purified enzyme, using 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) as substrate, at pH 3.0 and 50°C Pleurotus ostreatus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) + O2
-
 Pleurotus ostreatus ?
-
 ?
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) + O2
-
 Pleurotus ostreatus 43 BIM F-306D ?
-
 ?
2,6-dimethoxyphenol + O2
-
 Pleurotus ostreatus ?
-
 ?
2,6-dimethoxyphenol + O2
-
 Pleurotus ostreatus 43 BIM F-306D ?
-
 ?
guaiacol + O2
-
 Pleurotus ostreatus ?
-
 ?
guaiacol + O2
-
 Pleurotus ostreatus 43 BIM F-306D ?
-
 ?
additional information the isolated enzyme from Pleurotus ostreatus mycelium has both bilirubin oxidase and laccase activities Pleurotus ostreatus ?
-
 ?
additional information the isolated enzyme from Pleurotus ostreatus mycelium has both bilirubin oxidase and laccase activities Pleurotus ostreatus 43 BIM F-306D ?
-
 ?
syringaldazine + O2
-
 Pleurotus ostreatus ?
-
 ?
syringaldazine + O2
-
 Pleurotus ostreatus 43 BIM F-306D ?
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 59000, isoform POXC, SDS-PAGE Pleurotus ostreatus
monomer 1 * 61000, isoform POXA1w, SDS-PAGE Pleurotus ostreatus
monomer 1 * 62000, isoform POXA1b, SDS-PAGE Pleurotus ostreatus
monomer 1 * 67000, isoform POXA2, SDS-PAGE Pleurotus ostreatus
monomer 1 * 83000-85000, isoforms POXA3a and POXA3b, SDS-PAGE Pleurotus ostreatus

Synonyms

Synonyms Comment Organism
p-diphenol:oxygen oxidoreductase
-
 Pleurotus ostreatus
POXA1b isoform Pleurotus ostreatus
POXA1w isoform Pleurotus ostreatus
POXA2 isoform Pleurotus ostreatus
POXA3a isoform Pleurotus ostreatus
POXA3b isoform Pleurotus ostreatus
POXC isoform Pleurotus ostreatus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
20 50 isoform POXA1b Pleurotus ostreatus
25 35 isoform POXA2 Pleurotus ostreatus
35
-
 isoforms POXA3a and POXA3b Pleurotus ostreatus
45 65 isoform POXA1w Pleurotus ostreatus
50 60 isoform POXC Pleurotus ostreatus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
40 60 isoform POXA1b shows a inactivation half-period of 3 h at 60°C, isoform POXA1w shows a inactivation half-period of 3.3 h at 60°C, isoform POXA2 shows a inactivation half-period of 0.2 h at 60°C, isoform POXA3a shows a inactivation half-period of 6 h at 40°C, isoform POXA3b shows a inactivation half-period of 14 h at 40°C, isoform POXC shows a inactivation half-period of 30 min at 40°C Pleurotus ostreatus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
3 6.5 using 2,6-dimethoxyphenol as substrate Pleurotus ostreatus
3 3.6 using 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) as substrate Pleurotus ostreatus
6 7 using syringaldazine or guaiacol as substrate Pleurotus ostreatus