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Literature summary for 1.10.3.2 extracted from

  • Wong, J.H.; Ng, T.B.; Jiang, Y.; Liu, F.; Cho, S.; Sze, W.; Zhang, K.Y.
    Purification and characterization of a laccase with inhibitory activity toward HIV-1 reverse transcriptase and tumor cells from an edible mushroom (Pleurotus cornucopiae) (2010), Protein Pept. Lett., 17, 1040-1047.
    View publication on PubMed
Search for more literature for 1.10.3.2

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
66000
-
 x * 66000 Pleurotus cornucopiae

Organism

Organism UniProt Comment Textmining
Pleurotus cornucopiae
-
-
-

Purification (Commentary)

Purification (Comment) Organism
native enzyme 16fold from fresh fruitig bodies by affinity chromatography, ion exchange chromatography, and gel filtration Pleurotus cornucopiae

Source Tissue

Source Tissue Comment Organism Textmining
fruit body
-
 Pleurotus cornucopiae
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
17.3
-
 purified native enzyme Pleurotus cornucopiae

Subunits

Subunits Comment Organism
? x * 66000 Pleurotus cornucopiae

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
40
-
-
 Pleurotus cornucopiae

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
4
-
-
 Pleurotus cornucopiae

General Information

General Information Comment Organism
physiological function the enzyme inhibits proliferation of murine leukemia cell line L1210 and human heptoma cell line Hep-G2, and reduces the activity of HIV-1 reverse transcriptase, IC50 is 0.022 mM, but shows no mitogenic activity on mouse splenocytes, nor hemagglutinin/hemolytic activity towards rabbit erythrocytes Pleurotus cornucopiae