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Literature summary for 1.10.3.2 extracted from
- Production, purification and characterization of mid-redox potential laccase from a newly isolated Trichoderma harzianum WL1 (2008), Process Biochem., 43, 736-742.
No PubMed abstract available
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| azide | complete inhibition at 0.02 mM | Trichoderma harzianum |  |
| Cr3+ | inhibits 13.8% at 1 mM | Trichoderma harzianum | |
| diethyldithiocarbamic acid | - |
Trichoderma harzianum | |
| EDTA | - |
Trichoderma harzianum | |
| ethanol | - |
Trichoderma harzianum | |
| Hg2+ | inhibits 17.2% at 1 mM | Trichoderma harzianum | |
| additional information | no or poor inhibition by Co2+, Zn2+, Sn2+, Mn2+, Mg2+, K+, Fe2+, Ba2+, and Na+ | Trichoderma harzianum | |
| thioglycolic acid | inhibits 92.4% at 750 mM | Trichoderma harzianum | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.06 | - |
guaiacol | pH 4.5, 35°C | Trichoderma harzianum | |
| 0.18 | - |
2,2'-azino-bis(3-ethyl-benzothiazoline-6-sulfonic acid) | pH 4.5, 35°C | Trichoderma harzianum | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Cu2+ | - |
Trichoderma harzianum | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 79000 | - |
x * 79000, SDS-PAGE | Trichoderma harzianum |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Trichoderma harzianum | - |
- |
- |
| Trichoderma harzianum WL1 | - |
- |
- |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| glycoprotein | - |
Trichoderma harzianum |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| native enzyme 151.7fold by ultrafiltration, gel filtration, and concanavalin A affinity chromatography | Trichoderma harzianum |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 130.5 | - |
purified enzyme | Trichoderma harzianum |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) + O2 | - |
Trichoderma harzianum | ? | - |
? | |
| 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) + O2 | - |
Trichoderma harzianum WL1 | ? | - |
? | |
| guaiacol + O2 | - |
Trichoderma harzianum | ? | - |
? | |
| guaiacol + O2 | - |
Trichoderma harzianum WL1 | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 79000, SDS-PAGE | Trichoderma harzianum |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 35 | - |
- |
Trichoderma harzianum |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | 50 | activity range, rapid loss at 60°C | Trichoderma harzianum |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 35 | - |
purified enzyme, 24 h, stable | Trichoderma harzianum |
| 65 | - |
purified enzyme, half-life is 60 min | Trichoderma harzianum |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 4.5 | - |
- |
Trichoderma harzianum |
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