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Literature summary for 1.10.3.2 extracted from

  • Haibo, Z.; Yinglong, Z.; Feng, H.; Peiji, G.; Jiachuan, C.
    Purification and characterization of a thermostable laccase with unique oxidative characteristics from Trametes hirsuta (2009), Biotechnol. Lett., 31, 837-843.
    View publication on PubMed
Search for more literature for 1.10.3.2

Activating Compound

Activating Compound Comment Organism Structure
CuSO4 activates 113% at over 40 mM with substrate 3,6-dimethoxyphenol, and 46% at over 10 mM with substrate 2,2'-azinobis(3-ethylbenzo-6-thiazolinesulfonic) acid Trametes hirsuta
EDTA activates 10% at over 80 mM with substrate 3,6-dimethoxyphenol, and 50% at over 80 mM with substrate 2,2'-azinobis(3-ethylbenzo-6-thiazolinesulfonic) acid Trametes hirsuta
MnSO4 activates 49% at over 80 mM with substrate 3,6-dimethoxyphenol, and 28% at over 80 mM with substrate 2,2'-azinobis(3-ethylbenzo-6-thiazolinesulfonic) acid Trametes hirsuta
oxalic acid activates 42% at 2 mM with substrate 3,6-dimethoxyphenol, and 303% at 20 mM with substrate 2,2'-azinobis(3-ethylbenzo-6-thiazolinesulfonic) acid Trametes hirsuta

Inhibitors

Inhibitors Comment Organism Structure
DTT complete inhibition at over 10 mM with substrate 3,6-dimethoxyphenol and at over 20 mM with substrate 2,2'-azinobis(3-ethylbenzo-6-thiazolinesulfonic) acid Trametes hirsuta
NaF complete inhibition at over 10 mM with substrates 3,6-dimethoxyphenol and 2,2'-azinobis(3-ethylbenzo-6-thiazolinesulfonic) acid Trametes hirsuta
NaN3 complete inhibition at over 80 mM with substrate 3,6-dimethoxyphenol and at over 40 mM with substrate 2,2'-azinobis(3-ethylbenzo-6-thiazolinesulfonic) acid Trametes hirsuta

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.07
-
 2,2'-azino-bis(3-ethyl-benzothiazoline-6-sulfonic acid) pH 4.8, 25°C Trametes hirsuta
0.2
-
 2,6-dimethoxyphenol pH 4.8, 25°C Trametes hirsuta

Metals/Ions

Metals/Ions Comment Organism Structure
Cu2+ a copper enzyme Trametes hirsuta
additional information the enzyme is not affected by Cu2+, Mn2+, or EDTA Trametes hirsuta

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
90000
-
 x * 90000, SDS-PAGE Trametes hirsuta

Organism

Organism UniProt Comment Textmining
Trametes hirsuta
-
 isolated from Mengshan Mountain in Shandong Province, China
-
Trametes hirsuta Ig-9 / CGMCC 2422
-
 isolated from Mengshan Mountain in Shandong Province, China
-

Purification (Commentary)

Purification (Comment) Organism
native enzyme 180fold by cold precipitation, ultrafiltration ammonium sulfate fractionation, anion exchange chromatography, and gel filtration Trametes hirsuta

Source Tissue

Source Tissue Comment Organism Textmining
mycelium
-
 Trametes hirsuta
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
360
-
 purified native enzyme Trametes hirsuta

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1,3,5-trihydroxybenzene + O2
-
 Trametes hirsuta ?
-
 ?
1,3,5-trihydroxybenzene + O2
-
 Trametes hirsuta Ig-9 / CGMCC 2422 ?
-
 ?
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) + O2 high activity Trametes hirsuta ?
-
 ?
2,6-dimethoxyphenol + O2
-
 Trametes hirsuta ?
-
 ?
2,6-dimethoxyphenol + O2
-
 Trametes hirsuta Ig-9 / CGMCC 2422 ?
-
 ?
3-aminobenzoic acid + O2 low activity Trametes hirsuta ?
-
 ?
alizarin red S + O2
-
 Trametes hirsuta ?
-
 ?
catechol + O2
-
 Trametes hirsuta ?
-
 ?
catechol + O2
-
 Trametes hirsuta Ig-9 / CGMCC 2422 ?
-
 ?
guaiacol + O2
-
 Trametes hirsuta ?
-
 ?
guaiacol + O2
-
 Trametes hirsuta Ig-9 / CGMCC 2422 ?
-
 ?
methyl red + O2
-
 Trametes hirsuta ?
-
 ?
additional information no activity with veratryl alcohol, phenol, and L-tyrosine, substrate specificity, overview Trametes hirsuta ?
-
 ?
additional information no activity with veratryl alcohol, phenol, and L-tyrosine, substrate specificity, overview Trametes hirsuta Ig-9 / CGMCC 2422 ?
-
 ?

Subunits

Subunits Comment Organism
? x * 90000, SDS-PAGE Trametes hirsuta

Synonyms

Synonyms Comment Organism
More the enzyme belongs to the multicopper oxidase family Trametes hirsuta

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
85
-
 substrate 2,6-dimethoxyphenol Trametes hirsuta

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
25 90
-
 Trametes hirsuta

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
65
-
 half-life is 5 h Trametes hirsuta
75
-
 half-life is 70 min Trametes hirsuta

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
50
-
 2,6-dimethoxyphenol pH 4.8, 25°C Trametes hirsuta
197
-
 2,2'-azino-bis(3-ethyl-benzothiazoline-6-sulfonic acid) pH 4.8, 25°C Trametes hirsuta

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4 2.5
-
 Trametes hirsuta

pH Range

pH Minimum pH Maximum Comment Organism
2.2 5 activity range Trametes hirsuta

pI Value

Organism Comment pI Value Maximum pI Value
Trametes hirsuta isoelectric focussing
-
 4.3