Activating Compound | Comment | Organism | Structure |
---|---|---|---|
CuSO4 | activates 113% at over 40 mM with substrate 3,6-dimethoxyphenol, and 46% at over 10 mM with substrate 2,2'-azinobis(3-ethylbenzo-6-thiazolinesulfonic) acid | Trametes hirsuta | |
EDTA | activates 10% at over 80 mM with substrate 3,6-dimethoxyphenol, and 50% at over 80 mM with substrate 2,2'-azinobis(3-ethylbenzo-6-thiazolinesulfonic) acid | Trametes hirsuta | |
MnSO4 | activates 49% at over 80 mM with substrate 3,6-dimethoxyphenol, and 28% at over 80 mM with substrate 2,2'-azinobis(3-ethylbenzo-6-thiazolinesulfonic) acid | Trametes hirsuta | |
oxalic acid | activates 42% at 2 mM with substrate 3,6-dimethoxyphenol, and 303% at 20 mM with substrate 2,2'-azinobis(3-ethylbenzo-6-thiazolinesulfonic) acid | Trametes hirsuta |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
DTT | complete inhibition at over 10 mM with substrate 3,6-dimethoxyphenol and at over 20 mM with substrate 2,2'-azinobis(3-ethylbenzo-6-thiazolinesulfonic) acid | Trametes hirsuta | |
NaF | complete inhibition at over 10 mM with substrates 3,6-dimethoxyphenol and 2,2'-azinobis(3-ethylbenzo-6-thiazolinesulfonic) acid | Trametes hirsuta | |
NaN3 | complete inhibition at over 80 mM with substrate 3,6-dimethoxyphenol and at over 40 mM with substrate 2,2'-azinobis(3-ethylbenzo-6-thiazolinesulfonic) acid | Trametes hirsuta |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.07 | - |
2,2'-azino-bis(3-ethyl-benzothiazoline-6-sulfonic acid) | pH 4.8, 25°C | Trametes hirsuta | |
0.2 | - |
2,6-dimethoxyphenol | pH 4.8, 25°C | Trametes hirsuta |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Cu2+ | a copper enzyme | Trametes hirsuta | |
additional information | the enzyme is not affected by Cu2+, Mn2+, or EDTA | Trametes hirsuta |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
90000 | - |
x * 90000, SDS-PAGE | Trametes hirsuta |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Trametes hirsuta | - |
isolated from Mengshan Mountain in Shandong Province, China | - |
Trametes hirsuta Ig-9 / CGMCC 2422 | - |
isolated from Mengshan Mountain in Shandong Province, China | - |
Purification (Comment) | Organism |
---|---|
native enzyme 180fold by cold precipitation, ultrafiltration ammonium sulfate fractionation, anion exchange chromatography, and gel filtration | Trametes hirsuta |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
mycelium | - |
Trametes hirsuta | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
360 | - |
purified native enzyme | Trametes hirsuta |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
1,3,5-trihydroxybenzene + O2 | - |
Trametes hirsuta | ? | - |
? | |
1,3,5-trihydroxybenzene + O2 | - |
Trametes hirsuta Ig-9 / CGMCC 2422 | ? | - |
? | |
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) + O2 | high activity | Trametes hirsuta | ? | - |
? | |
2,6-dimethoxyphenol + O2 | - |
Trametes hirsuta | ? | - |
? | |
2,6-dimethoxyphenol + O2 | - |
Trametes hirsuta Ig-9 / CGMCC 2422 | ? | - |
? | |
3-aminobenzoic acid + O2 | low activity | Trametes hirsuta | ? | - |
? | |
alizarin red S + O2 | - |
Trametes hirsuta | ? | - |
? | |
catechol + O2 | - |
Trametes hirsuta | ? | - |
? | |
catechol + O2 | - |
Trametes hirsuta Ig-9 / CGMCC 2422 | ? | - |
? | |
guaiacol + O2 | - |
Trametes hirsuta | ? | - |
? | |
guaiacol + O2 | - |
Trametes hirsuta Ig-9 / CGMCC 2422 | ? | - |
? | |
methyl red + O2 | - |
Trametes hirsuta | ? | - |
? | |
additional information | no activity with veratryl alcohol, phenol, and L-tyrosine, substrate specificity, overview | Trametes hirsuta | ? | - |
? | |
additional information | no activity with veratryl alcohol, phenol, and L-tyrosine, substrate specificity, overview | Trametes hirsuta Ig-9 / CGMCC 2422 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 90000, SDS-PAGE | Trametes hirsuta |
Synonyms | Comment | Organism |
---|---|---|
More | the enzyme belongs to the multicopper oxidase family | Trametes hirsuta |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
85 | - |
substrate 2,6-dimethoxyphenol | Trametes hirsuta |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | 90 | - |
Trametes hirsuta |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
65 | - |
half-life is 5 h | Trametes hirsuta |
75 | - |
half-life is 70 min | Trametes hirsuta |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
50 | - |
2,6-dimethoxyphenol | pH 4.8, 25°C | Trametes hirsuta | |
197 | - |
2,2'-azino-bis(3-ethyl-benzothiazoline-6-sulfonic acid) | pH 4.8, 25°C | Trametes hirsuta |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
2.4 | 2.5 | - |
Trametes hirsuta |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
2.2 | 5 | activity range | Trametes hirsuta |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Trametes hirsuta | isoelectric focussing | - |
4.3 |