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Literature summary for 1.10.3.2 extracted from

  • Komori, H.; Miyazaki, K.; Higuchi, Y.
    Crystallization and preliminary X-ray diffraction analysis of a putative two-domain-type laccase from a metagenome (2009), Acta Crystallogr. Sect. F, 65, 264-266.
    View publication on PubMedView publication on EuropePMC

Application

Application Comment Organism
additional information the enzyme may be used in a variety of biotechnological applications, including textile dye bleaching, pulp bleaching, bioremediation, polymer synthesis and biosensors Thermus thermophilus

Cloned(Commentary)

Cloned (Comment) Organism
expression of a His-tagged two-type domain laccase, lacking the signal sequence domain, in Escherichia coli strain BL21(DE3) Thermus thermophilus

Crystallization (Commentary)

Crystallization (Comment) Organism
two-domain-type by sitting-drop vapour-diffusion method, mixing of 0.001 ml of a protein solution, containing 8.5 mg/ml protein, with 0.001 ml of reservoir solution, containing 0.2 M potassium chloride, 0.05 M HEPES, pH 7.5, and 35% v/v pentaerythritol propoxylate, at 20°C, X-ray diffraction structure determination and analysis at 1.7 A resolution, single-wavelength anomalous diffraction technique using Cu atoms Thermus thermophilus

Protein Variants

Protein Variants Comment Organism
additional information construction of a two-type domain laccase from a three-type domain laccase by eliminating the signal sequence domain Thermus thermophilus

Metals/Ions

Metals/Ions Comment Organism Structure
Cu2+ a multicopper protein Thermus thermophilus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Thermus thermophilus the enzyme oxidizes a range of phenolic and nonphenolic compounds using molecular oxygen as an electron acceptor to produce water with a broad substrate specificity ?
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?

Organism

Organism UniProt Comment Textmining
Thermus thermophilus
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two-domain-type laccase from a metagenome
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Purification (Commentary)

Purification (Comment) Organism
recombinant His-tagged two-type domain laccase from Escherichia coli strain BL21(DE3) by heat treatment, centrifugation, nickel affinity chromatography, and ultrafiltration Thermus thermophilus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information the enzyme oxidizes a range of phenolic and nonphenolic compounds using molecular oxygen as an electron acceptor to produce water with a broad substrate specificity Thermus thermophilus ?
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?

Synonyms

Synonyms Comment Organism
Benzenediol:oxygen oxidoreductase
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Thermus thermophilus