KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.018 | - |
syringaldazine | pH 7, holoCotA | Bacillus subtilis | |
0.056 | - |
K4Fe(CN)6 | pH 3, holoCotA | Bacillus subtilis | |
0.124 | - |
2,2'-azinobis(3-ethylbenzthiazoline-6-sulfonate) | pH 3, holoCotA | Bacillus subtilis | |
0.216 | - |
2,6-dimethoxyphenol | pH 7, holoCotA | Bacillus subtilis |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
copper | copper content of recombinant CotA laccase from Bacillus subtilis produced by Escherichia coli cells is shown to be strongly dependent on the presence of copper and oxygen in the culture media. In copper-supplemented media, a switch from aerobic to microaerobic conditions leads to the synthesis of a recombinant holoenzyme, while the maintenance of aerobic conditions results in the synthesis of a copper-depleted population of proteins. Strikingly, cells grown under microaerobic conditions accumulate up to 80fold more copper than aerobically grown cells. In vitro copper incorporation into apoenzymes is monitored by optical and electron paramagnetic resonance spectroscopy. This analysis reveals that copper incorporation into CotA laccase is a sequential process, with the type 1 copper center being the first to be reconstituted, followed by the type 2 and the type 3 copper centers | Bacillus subtilis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus subtilis | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Bacillus subtilis |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
1.52 | - |
recombinant enzyme from Escherichia coli AH3517 cells grown under aerobic conditions | Bacillus subtilis |
228.2 | - |
recombinant enzyme from Escherichia coli AH3517 cells grown under microaerobic conditions | Bacillus subtilis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2,2'-azinobis(3-ethylbenzthiazoline-6-sulfonate) + O2 | - |
Bacillus subtilis | ? | - |
? | |
2,6-dimethoxyphenol + O2 | - |
Bacillus subtilis | ? | - |
? | |
K4Fe(CN)6 + O2 | - |
Bacillus subtilis | ? | - |
? | |
syringaldazine + O2 | - |
Bacillus subtilis | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
CotA laccase | - |
Bacillus subtilis |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
29 | - |
2,6-dimethoxyphenol | pH 7, holoCotA | Bacillus subtilis | |
80 | - |
syringaldazine | pH 7, holoCotA | Bacillus subtilis | |
322 | - |
2,2'-azinobis(3-ethylbenzthiazoline-6-sulfonate) | pH 3, holoCotA | Bacillus subtilis | |
529 | - |
K4Fe(CN)6 | pH 3, holoCotA | Bacillus subtilis |