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Literature summary for 1.10.3.2 extracted from

  • Martin, C.; Pecyna, M.; Kellner, H.; Jehmlich, N.; Junghanns, C.; Benndorf, D.; von Bergen, M.; Schlosser, D.
    Purification and biochemical characterization of a laccase from the aquatic fungus Myrioconium sp. UHH 1-13-18-4 and molecular analysis of the laccase-encoding gene (2007), Appl. Microbiol. Biotechnol., 77, 613-624.
    View publication on PubMed
Search for more literature for 1.10.3.2

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0042
-
 syringaldazine 25°C, pH 6 Myrioconium sp.
0.0678
-
 2,6-dimethoxyphenol 25°C, pH 6 Myrioconium sp.
0.1049
-
 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonate) 25°C, pH 4 Myrioconium sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
72700
-
 matrix-assisted laser desorption/ionization-time of flightmass spectrometry Myrioconium sp.

Organism

Organism UniProt Comment Textmining
Myrioconium sp.
-
-
-
Myrioconium sp. UHH 1-13-18-4
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Myrioconium sp.

Source Tissue

Source Tissue Comment Organism Textmining
culture condition fungal cultures grown in malt extract medium Myrioconium sp.
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
80
-
-
 Myrioconium sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonate) + O2
-
 Myrioconium sp. ?
-
 ?
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonate) + O2
-
 Myrioconium sp. UHH 1-13-18-4 ?
-
 ?
2,6-dimethoxyphenol + O2
-
 Myrioconium sp. ?
-
 ?
2,6-dimethoxyphenol + O2
-
 Myrioconium sp. UHH 1-13-18-4 ?
-
 ?
syringaldazine + O2
-
 Myrioconium sp. ?
-
 ?
syringaldazine + O2
-
 Myrioconium sp. UHH 1-13-18-4 ?
-
 ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
70
-
 oxidation of 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonate) Myrioconium sp.

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
30 70 30°C: about 75% of maximal activity, 70°C: optimum, oxidation of 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonate) Myrioconium sp.

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.29
-
 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonate) 25°C, pH 4 Myrioconium sp.
2.96
-
 syringaldazine 25°C, pH 6 Myrioconium sp.
3.18
-
 2,6-dimethoxyphenol 25°C, pH 6 Myrioconium sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
2.5 3 oxidation of 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonate) Myrioconium sp.
6
-
 oxidation of syringaldazine Myrioconium sp.
6.5
-
 oxidation of 2,6-dimethoxyphenol Myrioconium sp.

pH Range

pH Minimum pH Maximum Comment Organism
2 5.5 pH 2.0: about 65% of maximal activity, pH 5.5: about 45% of maximal activity, oxidation of 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonate) Myrioconium sp.
5.5 6.5 pH 5.5: about 90% of maximal activity, pH 6.5: about 60% of maximal activity, oxidation of syringaldazine Myrioconium sp.

pH Stability

pH Stability pH Stability Maximum Comment Organism
4 6 15°C, 1 h, more than 80% of the initial laccase activity is retained Myrioconium sp.

pI Value

Organism Comment pI Value Maximum pI Value
Myrioconium sp. isoelectric focusing
-
 2.8