Any feedback?
Literature summary for 1.10.3.2 extracted from
- Kinetic studies on the reaction between Trametes villosa laccase and dioxygen (2006), J. Inorg. Biochem., 100, 1547-1557.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| gene lcc1, expression in Aspergillus oryzae as precursor enzyme | Trametes villosa |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | transient/stopped-flow and steady-state kinetics, modeling | Trametes villosa |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Cu2+ | blue multi-copper oxidase | Trametes villosa |  |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 63000 | - |
2 * 63000, recombinant enzyme, SDS-PAGE | Trametes villosa |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Trametes villosa | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant enzyme from Aspergillus oryzae by gel filtration, anion exchange chromatography, ammonium sulfate fractionation, hydrophobic interaction chromatography, and ultrafiltration | Trametes villosa |
Reaction
| Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|
| 4 benzenediol + O2 = 4 benzosemiquinone + 2 H2O | reaction mechanism of the reduction of dioxygen to water, second-order rate constant for the reaction with dioxygen, ping-pong mechanism, kinetic model | Trametes villosa |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 4-hydroxybenzoic acid + O2 | - |
Trametes villosa | ? | - |
? | |
| 4-hydroxyphenylacetic acid + O2 | second-order rate constant for the reaction with dioxygen | Trametes villosa | ? | - |
? | |
| guaiacolsulfonic acid + O2 | - |
Trametes villosa | ? | - |
? | |
| hydroquinone + O2 | - |
Trametes villosa | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 2 * 63000, recombinant enzyme, SDS-PAGE | Trametes villosa |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 25 | - |
assay at | Trametes villosa |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 10 | 35 | - |
Trametes villosa |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5 | - |
assay at | Trametes villosa |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Trametes villosa | isoelectric focusing | - |
3.5 |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
