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Literature summary for 1.10.3.2 extracted from

  • Vasdev, K.; Dhawan, S.; Kapoor, R.K.; Kuhad, R.C.
    Biochemical characterization and molecular evidence of a laccase from the birds nest fungus Cyathus bulleri (2005), Fungal Genet. Biol., 42, 684-693.
    View publication on PubMed
Search for more literature for 1.10.3.2

Cloned(Commentary)

Cloned (Comment) Organism
DNA and amino acid sequence determination and analysis Cyathus bulleri

Inhibitors

Inhibitors Comment Organism Structure
2-mercaptoethanol competitive, complete inhibition Cyathus bulleri
cetyltrimethyl ammonium bromide 50% inhibition Cyathus bulleri
HgCl2 competitive, complete inhibition Cyathus bulleri
KCN competitive, complete inhibition Cyathus bulleri
NaN3 competitive, complete inhibition Cyathus bulleri
SDS competitive, complete inhibition Cyathus bulleri

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
14
-
 ferulic acid pH 5.2, 45°C Cyathus bulleri
22
-
 guaiacol pH 5.2, 45°C Cyathus bulleri
48.6
-
 2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) pH 5.2, 45°C Cyathus bulleri
56
-
 2,6-dimethoxyphenol pH 5.2, 45°C Cyathus bulleri

Metals/Ions

Metals/Ions Comment Organism Structure
Cu2+ the gene sequence encodes copper-binding domains I and II Cyathus bulleri
additional information no effect by EDTA Cyathus bulleri

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
60000
-
 gel filtration Cyathus bulleri

Organism

Organism UniProt Comment Textmining
Cyathus bulleri Q8X1D1 birds nest fungus
-

Purification (Commentary)

Purification (Comment) Organism
native enzyme 95.7fold to homogeneity by anion exchange chromatography and gel filtration Cyathus bulleri

Source Tissue

Source Tissue Comment Organism Textmining
mycelium
-
 Cyathus bulleri
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
226
-
 purified enzyme Cyathus bulleri

Storage Stability

Storage Stability Organism
4°C, purified enzyme, 50 mM citrate phosphate, pH 5.0, no loss in activity after 1 month Cyathus bulleri

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) + O2
-
 Cyathus bulleri ?
-
 ?
2,6-dimethoxyphenol + O2
-
 Cyathus bulleri 3,3',5,5'-tetramethoxy-4-diphenoquinone + H2O
-
 ?
3,4-dihydroxybenzoic acid + O2
-
 Cyathus bulleri ?
-
 ?
ferulic acid + O2
-
 Cyathus bulleri ?
-
 ?
guaiacol + O2
-
 Cyathus bulleri ?
-
 ?
additional information substrate specificity, overview, no activity with tyrosine, phenol, 2,4-dichlorophenol, and 5-sulphosalycylic acid Cyathus bulleri ?
-
 ?
syringaldehyde + O2
-
 Cyathus bulleri ?
-
 ?
vanillin + O2
-
 Cyathus bulleri ?
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 60000, SDS-PAGE Cyathus bulleri

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
45
-
-
 Cyathus bulleri

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
25 70 stable at, inactivation above Cyathus bulleri
80
-
 complete inactivation Cyathus bulleri

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
5.2
-
 ferulic acid pH 5.2, 45°C Cyathus bulleri
5.5
-
 guaiacol pH 5.2, 45°C Cyathus bulleri
95.6
-
 guaiacol pH 5.2, 45°C Cyathus bulleri
180
-
 2,6-dimethoxyphenol pH 5.2, 45°C Cyathus bulleri
204
-
 2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) pH 5.2, 45°C Cyathus bulleri

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.2
-
-
 Cyathus bulleri

pH Range

pH Minimum pH Maximum Comment Organism
2 6 sharp decline in activity above pH 6.0 Cyathus bulleri

pI Value

Organism Comment pI Value Maximum pI Value
Cyathus bulleri isoelectric focusing
-
 3.7