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Literature summary for 1.10.3.2 extracted from

  • Michniewicz, A.; Ullrich, R.; Ledakowicz, S.; Hofrichter, M.
    The white-rot fungus Cerrena unicolor strain 137 produces two laccase isoforms with different physico-chemical and catalytic properties (2006), Appl. Microbiol. Biotechnol., 69, 682-688.
    View publication on PubMed

Metals/Ions

Metals/Ions Comment Organism Structure
Cu2+ required, stimulates up to 0.2 mM, contains type I Cu+ and type III Cu+ Cerrena unicolor

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
57000
-
1 * 64000, isozyme Lacc I, SDS-PAGE, 1 * 57000, isozyme Lacc II, SDS-PAGE Cerrena unicolor
64000
-
1 * 64000, isozyme Lacc I, SDS-PAGE, 1 * 57000, isozyme Lacc II, SDS-PAGE Cerrena unicolor

Organism

Organism UniProt Comment Textmining
Cerrena unicolor
-
two isozymes Lacc I and Lacc II
-
Cerrena unicolor 137
-
two isozymes Lacc I and Lacc II
-

Purification (Commentary)

Purification (Comment) Organism
native enzyme 26fold by ultrafiltration/diafiltration, gel filtration and anion exchange chromatography Cerrena unicolor

Source Tissue

Source Tissue Comment Organism Textmining
additional information high production rate culture, overview Cerrena unicolor
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
Cerrena unicolor
206
-
purified enzyme Cerrena unicolor

Storage Stability

Storage Stability Organism
4°C, purified isozymes in 10 mM Na acetate buffer, pH 5.0, isozyme Lacc II is stable for at least 6 months, isozyme Lacc I shows 40% remaining activity after 6 months Cerrena unicolor

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) + O2
-
Cerrena unicolor ?
-
?
2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) + O2
-
Cerrena unicolor 137 ?
-
?
2,6-dimethoxyphenol + O2
-
Cerrena unicolor 3,3',5,5'-tetramethoxy-4-diphenoquinone + H2O
-
?
2,6-dimethoxyphenol + O2
-
Cerrena unicolor 137 3,3',5,5'-tetramethoxy-4-diphenoquinone + H2O
-
?
syringaldazine + O2
-
Cerrena unicolor ?
-
?
syringaldazine + O2
-
Cerrena unicolor 137 ?
-
?

Subunits

Subunits Comment Organism
monomer 1 * 64000, isozyme Lacc I, SDS-PAGE, 1 * 57000, isozyme Lacc II, SDS-PAGE Cerrena unicolor

Synonyms

Synonyms Comment Organism
Lacc
-
Cerrena unicolor

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
assay at Cerrena unicolor

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
5 75 high activity at 5°C, 25 and 30% of maximum activity at 60°C for isozymes Lacc I and II, 20% at 75°C Cerrena unicolor

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
25
-
purified isozymes Lacc I and Lacc II are stable Cerrena unicolor
50
-
60 min, purified isozyme Lacc I shows loss of 20% activity, purified isozyme Lacc II is stable Cerrena unicolor
70
-
20 min, purified isozyme Lacc I shows complete loss of activity, isozyme Lacc II 10% after 60 min Cerrena unicolor
80
-
rapid inactivation of purified isozyme Lacc II with 95% loss of activity within 10 min Cerrena unicolor

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
3.5 5.5 dependent on the substrate Cerrena unicolor

pH Range

pH Minimum pH Maximum Comment Organism
2.5 5.5 inactivation of isozyme Lacc I at pH 6.5, of isozyme Lacc II at pH 7.5 Cerrena unicolor

pH Stability

pH Stability pH Stability Maximum Comment Organism
3
-
partial inactivation of both isozymes, 60-64% remaining activity after 1 h Cerrena unicolor
7
-
isozyme Lacc I and isozyme Lacc II, stable Cerrena unicolor
10
-
isozyme Lacc II is stable, isozyme Lacc I loses 10% of activity within 1 h Cerrena unicolor

pI Value

Organism Comment pI Value Maximum pI Value
Cerrena unicolor isozyme Lacc I, isoelectric focusing
-
3.6
Cerrena unicolor isozyme Lacc II, isoelectric focusing
-
3.7