Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Cu2+ | required, stimulates up to 0.2 mM, contains type I Cu+ and type III Cu+ | Cerrena unicolor |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
57000 | - |
1 * 64000, isozyme Lacc I, SDS-PAGE, 1 * 57000, isozyme Lacc II, SDS-PAGE | Cerrena unicolor |
64000 | - |
1 * 64000, isozyme Lacc I, SDS-PAGE, 1 * 57000, isozyme Lacc II, SDS-PAGE | Cerrena unicolor |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Cerrena unicolor | - |
two isozymes Lacc I and Lacc II | - |
Cerrena unicolor 137 | - |
two isozymes Lacc I and Lacc II | - |
Purification (Comment) | Organism |
---|---|
native enzyme 26fold by ultrafiltration/diafiltration, gel filtration and anion exchange chromatography | Cerrena unicolor |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
additional information | high production rate culture, overview | Cerrena unicolor | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Cerrena unicolor |
206 | - |
purified enzyme | Cerrena unicolor |
Storage Stability | Organism |
---|---|
4°C, purified isozymes in 10 mM Na acetate buffer, pH 5.0, isozyme Lacc II is stable for at least 6 months, isozyme Lacc I shows 40% remaining activity after 6 months | Cerrena unicolor |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) + O2 | - |
Cerrena unicolor | ? | - |
? | |
2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) + O2 | - |
Cerrena unicolor 137 | ? | - |
? | |
2,6-dimethoxyphenol + O2 | - |
Cerrena unicolor | 3,3',5,5'-tetramethoxy-4-diphenoquinone + H2O | - |
? | |
2,6-dimethoxyphenol + O2 | - |
Cerrena unicolor 137 | 3,3',5,5'-tetramethoxy-4-diphenoquinone + H2O | - |
? | |
syringaldazine + O2 | - |
Cerrena unicolor | ? | - |
? | |
syringaldazine + O2 | - |
Cerrena unicolor 137 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 64000, isozyme Lacc I, SDS-PAGE, 1 * 57000, isozyme Lacc II, SDS-PAGE | Cerrena unicolor |
Synonyms | Comment | Organism |
---|---|---|
Lacc | - |
Cerrena unicolor |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at | Cerrena unicolor |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
5 | 75 | high activity at 5°C, 25 and 30% of maximum activity at 60°C for isozymes Lacc I and II, 20% at 75°C | Cerrena unicolor |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
purified isozymes Lacc I and Lacc II are stable | Cerrena unicolor |
50 | - |
60 min, purified isozyme Lacc I shows loss of 20% activity, purified isozyme Lacc II is stable | Cerrena unicolor |
70 | - |
20 min, purified isozyme Lacc I shows complete loss of activity, isozyme Lacc II 10% after 60 min | Cerrena unicolor |
80 | - |
rapid inactivation of purified isozyme Lacc II with 95% loss of activity within 10 min | Cerrena unicolor |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
3.5 | 5.5 | dependent on the substrate | Cerrena unicolor |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
2.5 | 5.5 | inactivation of isozyme Lacc I at pH 6.5, of isozyme Lacc II at pH 7.5 | Cerrena unicolor |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
3 | - |
partial inactivation of both isozymes, 60-64% remaining activity after 1 h | Cerrena unicolor |
7 | - |
isozyme Lacc I and isozyme Lacc II, stable | Cerrena unicolor |
10 | - |
isozyme Lacc II is stable, isozyme Lacc I loses 10% of activity within 1 h | Cerrena unicolor |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Cerrena unicolor | isozyme Lacc I, isoelectric focusing | - |
3.6 |
Cerrena unicolor | isozyme Lacc II, isoelectric focusing | - |
3.7 |