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Literature summary for 1.10.3.2 extracted from

  • Quaratino, D.; Federici, F.; Petruccioli, M.; Fenice, M.; D'Annibale, A.
    Production, purification and partial characterisation of a novel laccase from the white-rot fungus Panus tigrinus CBS 577.79 (2007), Antonie van Leeuwenhoek, 91, 57-69.
    View publication on PubMed

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular
-
Lentinus tigrinus
-
-

Metals/Ions

Metals/Ions Comment Organism Structure
Cu2+ presence of type-1 site and type-3 binuclear copper in the bright blue colored purified enzyme Lentinus tigrinus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
68500
-
1 * 68500, SDS-PAGE Lentinus tigrinus
69100
-
gel filtration Lentinus tigrinus

Organism

Organism UniProt Comment Textmining
Lentinus tigrinus
-
-
-
Lentinus tigrinus CBS 577.79
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein 6.9% N-glycosylation of the high mannose type, lectin-binding assays Lentinus tigrinus

Purification (Commentary)

Purification (Comment) Organism
native enzyme 16.7fold from strain 577.79 by ultrafiltration, anion exchange chromatography, and gel filtration to homogeneity Lentinus tigrinus

Source Tissue

Source Tissue Comment Organism Textmining
additional information produced in a bubble column reactor using glucose-containing medium supplemented with 2,5-xylidine under conditions of nitrogen sufficiency Lentinus tigrinus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.71
-
crude extracellular enzyme in culture Lentinus tigrinus
317
-
purified enzyme Lentinus tigrinus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1-hydroxybenzotriazole + O2
-
Lentinus tigrinus ?
-
?
1-hydroxybenzotriazole + O2
-
Lentinus tigrinus CBS 577.79 ?
-
?
2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) + O2
-
Lentinus tigrinus ?
-
?
2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) + O2
-
Lentinus tigrinus CBS 577.79 ?
-
?
2,2,6,6-tetramethylpiperidin-N-oxide radical + O2
-
Lentinus tigrinus ?
-
?
2,2,6,6-tetramethylpiperidin-N-oxide radical + O2
-
Lentinus tigrinus CBS 577.79 ?
-
?
2,6-dimethoxyphenol + O2
-
Lentinus tigrinus 3,3',5,5'-tetramethoxy-4-diphenoquinone + H2O i.e. cerulignone ?
2-methoxyphenol + O2
-
Lentinus tigrinus ?
-
?
4-methoxyphenol + O2
-
Lentinus tigrinus ?
-
?
4-methyl catechol + O2
-
Lentinus tigrinus ?
-
?
catechol + O2
-
Lentinus tigrinus ?
-
?
catechol + O2
-
Lentinus tigrinus CBS 577.79 ?
-
?
additional information substrate specificity, overview Lentinus tigrinus ?
-
?
additional information substrate specificity, overview Lentinus tigrinus CBS 577.79 ?
-
?
violuric acid + O2
-
Lentinus tigrinus ?
-
?

Subunits

Subunits Comment Organism
monomer 1 * 68500, SDS-PAGE Lentinus tigrinus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
55
-
-
Lentinus tigrinus

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
25 55 temperature-activity profile at pH 3.8 Lentinus tigrinus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
50
-
purified enzyme, half-life: 281 min Lentinus tigrinus
60
-
purified enzyme, half-life: 25 min Lentinus tigrinus
70
-
purified enzyme, half-life: 4 min Lentinus tigrinus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.6
-
1-hydroxybenzotriazole pH 3.8, 55°C Lentinus tigrinus
7.8
-
2,2,6,6-tetramethylpiperidin-N-oxide radical pH 3.8, 55°C Lentinus tigrinus
8.4
-
violuric acid pH 3.8, 55°C Lentinus tigrinus
17.7
-
2,6-dimethoxyphenol pH 3.8, 55°C Lentinus tigrinus
22.8
-
2-methoxyphenol pH 3.8, 55°C Lentinus tigrinus
34.4
-
catechol pH 3.8, 55°C Lentinus tigrinus
36.3
-
4-methoxyphenol pH 3.8, 55°C Lentinus tigrinus
93.9
-
4-methyl catechol pH 3.8, 55°C Lentinus tigrinus
365.6
-
2,6-dimethoxyphenol pH 3.8, 55°C Lentinus tigrinus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
3.7 3.8
-
Lentinus tigrinus

pH Range

pH Minimum pH Maximum Comment Organism
2.5 6.5 3% of maximal activity at pH 6.5, pH-activity profile at 30°C Lentinus tigrinus

pH Stability

pH Stability pH Stability Maximum Comment Organism
additional information
-
-
Lentinus tigrinus

pI Value

Organism Comment pI Value Maximum pI Value
Lentinus tigrinus isoelectric focusing
-
3.15