Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
extracellular | - |
Lentinus tigrinus | - |
- |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Cu2+ | presence of type-1 site and type-3 binuclear copper in the bright blue colored purified enzyme | Lentinus tigrinus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
68500 | - |
1 * 68500, SDS-PAGE | Lentinus tigrinus |
69100 | - |
gel filtration | Lentinus tigrinus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Lentinus tigrinus | - |
- |
- |
Lentinus tigrinus CBS 577.79 | - |
- |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
glycoprotein | 6.9% N-glycosylation of the high mannose type, lectin-binding assays | Lentinus tigrinus |
Purification (Comment) | Organism |
---|---|
native enzyme 16.7fold from strain 577.79 by ultrafiltration, anion exchange chromatography, and gel filtration to homogeneity | Lentinus tigrinus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
additional information | produced in a bubble column reactor using glucose-containing medium supplemented with 2,5-xylidine under conditions of nitrogen sufficiency | Lentinus tigrinus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
1.71 | - |
crude extracellular enzyme in culture | Lentinus tigrinus |
317 | - |
purified enzyme | Lentinus tigrinus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
1-hydroxybenzotriazole + O2 | - |
Lentinus tigrinus | ? | - |
? | |
1-hydroxybenzotriazole + O2 | - |
Lentinus tigrinus CBS 577.79 | ? | - |
? | |
2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) + O2 | - |
Lentinus tigrinus | ? | - |
? | |
2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) + O2 | - |
Lentinus tigrinus CBS 577.79 | ? | - |
? | |
2,2,6,6-tetramethylpiperidin-N-oxide radical + O2 | - |
Lentinus tigrinus | ? | - |
? | |
2,2,6,6-tetramethylpiperidin-N-oxide radical + O2 | - |
Lentinus tigrinus CBS 577.79 | ? | - |
? | |
2,6-dimethoxyphenol + O2 | - |
Lentinus tigrinus | 3,3',5,5'-tetramethoxy-4-diphenoquinone + H2O | i.e. cerulignone | ? | |
2-methoxyphenol + O2 | - |
Lentinus tigrinus | ? | - |
? | |
4-methoxyphenol + O2 | - |
Lentinus tigrinus | ? | - |
? | |
4-methyl catechol + O2 | - |
Lentinus tigrinus | ? | - |
? | |
catechol + O2 | - |
Lentinus tigrinus | ? | - |
? | |
catechol + O2 | - |
Lentinus tigrinus CBS 577.79 | ? | - |
? | |
additional information | substrate specificity, overview | Lentinus tigrinus | ? | - |
? | |
additional information | substrate specificity, overview | Lentinus tigrinus CBS 577.79 | ? | - |
? | |
violuric acid + O2 | - |
Lentinus tigrinus | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 68500, SDS-PAGE | Lentinus tigrinus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
55 | - |
- |
Lentinus tigrinus |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | 55 | temperature-activity profile at pH 3.8 | Lentinus tigrinus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
purified enzyme, half-life: 281 min | Lentinus tigrinus |
60 | - |
purified enzyme, half-life: 25 min | Lentinus tigrinus |
70 | - |
purified enzyme, half-life: 4 min | Lentinus tigrinus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
2.6 | - |
1-hydroxybenzotriazole | pH 3.8, 55°C | Lentinus tigrinus | |
7.8 | - |
2,2,6,6-tetramethylpiperidin-N-oxide radical | pH 3.8, 55°C | Lentinus tigrinus | |
8.4 | - |
violuric acid | pH 3.8, 55°C | Lentinus tigrinus | |
17.7 | - |
2,6-dimethoxyphenol | pH 3.8, 55°C | Lentinus tigrinus | |
22.8 | - |
2-methoxyphenol | pH 3.8, 55°C | Lentinus tigrinus | |
34.4 | - |
catechol | pH 3.8, 55°C | Lentinus tigrinus | |
36.3 | - |
4-methoxyphenol | pH 3.8, 55°C | Lentinus tigrinus | |
93.9 | - |
4-methyl catechol | pH 3.8, 55°C | Lentinus tigrinus | |
365.6 | - |
2,6-dimethoxyphenol | pH 3.8, 55°C | Lentinus tigrinus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
3.7 | 3.8 | - |
Lentinus tigrinus |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
2.5 | 6.5 | 3% of maximal activity at pH 6.5, pH-activity profile at 30°C | Lentinus tigrinus |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Lentinus tigrinus |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Lentinus tigrinus | isoelectric focusing | - |
3.15 |