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Literature summary for 1.10.3.2 extracted from

  • Nagai, M.; Kawata, M.; Watanabe, H.; Ogawa, M.; Saito, K.; Takesawa, T.; Kanda, K.; Sato, T.
    Important role of fungal intracellular laccase for melanin synthesis: purification and characterization of an intracellular laccase from Lentinula edodes fruit bodies (2003), Microbiology, 149, 2455-2462.
    View publication on PubMed
Search for more literature for 1.10.3.2

Inhibitors

Inhibitors Comment Organism Structure
HgCl2 1 mM, 67% residual activity Lentinula edodes
MnCl2 1 mM, 83% residual activity Lentinula edodes
SnCl2 1 mM, 69% residual activity Lentinula edodes

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.127
-
 2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) 30°C, pH 3.0 Lentinula edodes
0.35
-
 guaiacol 30°C, pH 4.0 Lentinula edodes
0.351
-
 2,6-dimethoxyphenol 30°C, pH 3.0 Lentinula edodes
1.22
-
 beta-(3,4-dihydroxyphenyl)alanine 30°C, pH 4.0 Lentinula edodes
1.39
-
 ferulic acid 30°C, pH 5.0 Lentinula edodes
1.72
-
 catechol 30°C, pH 3.0 Lentinula edodes
1.72
-
 4-phenylenediamine 30°C, pH 4.0 Lentinula edodes
24.6
-
 pyrogallol 30°C, pH 3.0 Lentinula edodes

Localization

Localization Comment Organism GeneOntology No. Textmining

Metals/Ions

Metals/Ions Comment Organism Structure
copper
-
 Lentinula edodes

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
53000
-
 gel filtration Lentinula edodes
58000
-
 1 * 58000, SDS-PAGE Lentinula edodes

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Lentinula edodes involved in melanin synthesis ?
-
 ?

Organism

Organism UniProt Comment Textmining
Lentinula edodes
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein 8.6% carbohydrate content Lentinula edodes

Purification (Commentary)

Purification (Comment) Organism
-
 Lentinula edodes

Source Tissue

Source Tissue Comment Organism Textmining
fruit fully browned gill Lentinula edodes
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
305
-
 pH 3.0, 30°C Lentinula edodes

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) + O2
-
 Lentinula edodes ? + H2O
-
 ?
2,6-dimethoxyphenol + O2
-
 Lentinula edodes ? + H2O
-
 ?
4-phenylenediamine + O2
-
 Lentinula edodes ?
-
 r
beta-(3,4-dihydroxyphenyl)alanine + O2 i.e. L-dopa Lentinula edodes beta-(3,4-dihydroxyphenyl)alanine quinone + H2O
-
 ?
catechol + O2
-
 Lentinula edodes ? + H2O
-
 ?
ferulic acid + O2
-
 Lentinula edodes ? + H2O
-
 ?
guaiacol + O2
-
 Lentinula edodes ? + H2O
-
 ?
additional information involved in melanin synthesis Lentinula edodes ?
-
 ?
additional information no substrate: tyrosine, veratryl alcohol Lentinula edodes ?
-
 ?
p-phenylenediamine + O2
-
 Lentinula edodes ? + H2O
-
 ?
pyrogallol + O2
-
 Lentinula edodes ? + H2O
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 58000, SDS-PAGE Lentinula edodes

Synonyms

Synonyms Comment Organism
Lcc2
-
 Lentinula edodes

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
40
-
-
 Lentinula edodes

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
30
-
 pH 6.0, stable for 16 h Lentinula edodes
50
-
 stable up to Lentinula edodes
60
-
 30 min, 33% loss of activity Lentinula edodes

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
3
-
 substrates 2,2’-azinobis(3-ethylbenzothiazoline-6-sulfonic acid), 2,6-dimethoxyphenol, catechol, pyrogallol Lentinula edodes
4
-
 substrates guaiacol, beta-(3,4-dihydroxyphenyl)alanine, p-phenylendiamine Lentinula edodes
5
-
 substrate ferulic acid Lentinula edodes

pH Stability

pH Stability pH Stability Maximum Comment Organism
6
-
 30°C, stable for 16 h Lentinula edodes

pI Value

Organism Comment pI Value Maximum pI Value
Lentinula edodes isoelectric focusing
-
 7.1