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Literature summary for 1.10.3.2 extracted from

  • Ryan, S.; Schnitzhofer, W.; Tzanov, T.; Cavaco-Paulo, A.; Gubitz, G.M.
    An acid-stable laccase from Sclerotium rolfsii with potential for wool dye decolorization (2003), Enzyme Microb. Technol., 33, 766-774.
No PubMed abstract available
Search for more literature for 1.10.3.2

Application

Application Comment Organism
degradation use of enzyme to decolourize textile dye Athelia rolfsii

Inhibitors

Inhibitors Comment Organism Structure
diethyldithiocarbamate 50% inhibition at 1.2 mM Athelia rolfsii
EDTA 50% inhibition at about 300 mM Athelia rolfsii
ethanol 50% inhibition at 36% Athelia rolfsii
L-cysteine 50% inhibition at 0.52 mM Athelia rolfsii
methanol 50% inhibition at 37% Athelia rolfsii
Sodium azide 50% inhibition at 0.009 mM Athelia rolfsii
sodium bromide 50% inhibition at 195 mM Athelia rolfsii
sodium chloride 50% inhibition at 52 mM Athelia rolfsii
Sodium fluoride 50% inhibition at 0.08 mM Athelia rolfsii
thiamine 50% inhibition at 0.95 mM Athelia rolfsii
thioglycolic acid 50% inhibition at 0.5 mM Athelia rolfsii

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0168
-
 Diamond Black PV 200 25°C, pH 4.5 Athelia rolfsii

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
59000
-
 x * 59000, SDS-PAGE Athelia rolfsii

Organism

Organism UniProt Comment Textmining
Athelia rolfsii
-
 isozymes SRL1, induced by 2,5-xylidine with culture medium
-

Purification (Commentary)

Purification (Comment) Organism
isozyme SRL1 Athelia rolfsii

Source Tissue

Source Tissue Comment Organism Textmining
sclerotium
-
 Athelia rolfsii
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
14.7
-
 25°C, pH 4.5 Athelia rolfsii

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) + O2 82% of activity with syringaldazine Athelia rolfsii ? + H2O
-
 ?
2,6-dimethoxyphenol + O2 60% of activity with syringaldazine Athelia rolfsii ? + H2O
-
 ?
catechol + O2 11% of activity with syringaldazine Athelia rolfsii ? + H2O
-
 ?
Diamond Black PV 200 + ? i.e. Mordant Black 9, Eriochrome Black PV, wool azo dye Athelia rolfsii 2-amino-1-phenol-4-sulfonic acid + 1,5-naphthalenediol + ?
-
 ?
guaiacol + O2 21% of activity with syringaldazine Athelia rolfsii ? + H2O
-
 ?
Indigo + ? textile dye Athelia rolfsii ?
-
 ?
Lancet Marine Blue + ? textile dye Athelia rolfsii ?
-
 ?
lignin sulfonic acid + O2 0.9% of activity with syringaldazine Athelia rolfsii ? + H2O
-
 ?
pyrogallol + O2 7% of activity with syringaldazine Athelia rolfsii ? + H2O
-
 ?
Remazol Brilliand Red + ? textile dye Athelia rolfsii ?
-
 ?
syringaldazine + O2
-
 Athelia rolfsii ?
-
 ?

Subunits

Subunits Comment Organism
? x * 59000, SDS-PAGE Athelia rolfsii

Synonyms

Synonyms Comment Organism
SRL1
-
 Athelia rolfsii

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
62
-
-
 Athelia rolfsii

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
60
-
 pH 4.5, 60 min, 50% residual activity Athelia rolfsii
80
-
 pH 4.5, less than 2 min, 50% residual activity Athelia rolfsii

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
80.4
-
 Diamond Black PV 200 25°C, pH 4.5 Athelia rolfsii

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4
-
-
 Athelia rolfsii

pH Stability

pH Stability pH Stability Maximum Comment Organism
2.5
-
 30°C, 8 min, 50% residual activity Athelia rolfsii
3
-
 30°C, 12 min, 50% residual activity Athelia rolfsii
4
-
 18°C, 1 week, 50% residual activity Athelia rolfsii

pI Value

Organism Comment pI Value Maximum pI Value
Athelia rolfsii isoelectric focusing
-
 4.9