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Literature summary for 1.10.3.2 extracted from

  • Saito, T.; Hong, P.; Kato, K.; Okazaki, M.; Inagaki, H.; Maeda, S.; Yokogawa, Y.
    Purification and characterization of an extracellular laccase of a fungus (family Chaetomiaceae) isolated from soil (2003), Enzyme Microb. Technol., 33, 520-526.
No PubMed abstract available

Application

Application Comment Organism
degradation use of enzyme in biodegradation of endocrine-disrupting chemicals such as bisphenol A and nonylphenol Chaetomiaceae

Inhibitors

Inhibitors Comment Organism Structure
dithiothreitol
-
Chaetomiaceae
kojic acid
-
Chaetomiaceae
L-cysteine
-
Chaetomiaceae
p-coumaric acid
-
Chaetomiaceae
thioglycolic acid
-
Chaetomiaceae

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.009
-
syringaldazine pH 7.0, 30°C Chaetomiaceae
0.012
-
2,6-dimethoxyphenol pH 7.0, 30°C Chaetomiaceae
0.016
-
guaiacol pH 7.0, 30°C Chaetomiaceae
0.023
-
pyrogallol pH 7.0, 30°C Chaetomiaceae
0.123
-
hydroquinone pH 7.0, 30°C Chaetomiaceae
0.212
-
N,N-dimethyl-1,4-phenylenediamine pH 7.0, 30°C Chaetomiaceae
0.223
-
4-hydroxyindole pH 7.0, 30°C Chaetomiaceae
0.652
-
2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) pH 7.0, 30°C Chaetomiaceae
1.05
-
catechol pH 7.0, 30°C Chaetomiaceae

Metals/Ions

Metals/Ions Comment Organism Structure
copper 4 atoms per protein molecule, a type I CuII, a type II CuII and a type III binuclear CuII Chaetomiaceae

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
73000
-
1 * 73000, SDS-PAGE Chaetomiaceae
80000
-
gel filtration Chaetomiaceae

Organism

Organism UniProt Comment Textmining
Chaetomiaceae
-
fungus isolated from soil, growth in presence of caffeic acid
-

Purification (Commentary)

Purification (Comment) Organism
-
Chaetomiaceae

Source Tissue

Source Tissue Comment Organism Textmining
culture supernatant
-
Chaetomiaceae
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) + O2
-
Chaetomiaceae ? + H2O
-
?
2,6-dimethoxyphenol + O2
-
Chaetomiaceae ? + H2O
-
?
4-hydroxyindole + O2
-
Chaetomiaceae ? + H2O
-
?
catechol + O2
-
Chaetomiaceae ? + H2O
-
?
guaiacol + O2
-
Chaetomiaceae ? + H2O
-
?
hydroquinone + O2
-
Chaetomiaceae ? + H2O
-
?
N,N-dimethyl-1,4-phenylenediamine + O2
-
Chaetomiaceae ? + H2O
-
?
pyrogallol + O2
-
Chaetomiaceae ? + H2O
-
?
syringaldazine + O2
-
Chaetomiaceae ?
-
?

Subunits

Subunits Comment Organism
monomer 1 * 73000, SDS-PAGE Chaetomiaceae

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
42
-
-
Chaetomiaceae

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
4
-
stable up to 12 days Chaetomiaceae
25
-
150h, 50% residaul activity Chaetomiaceae
40
-
20h, 50% residual activity Chaetomiaceae

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3 6 guaiacol pH 7.0, 30°C Chaetomiaceae
4
-
syringaldazine pH 7.0, 30°C Chaetomiaceae
10
-
pyrogallol pH 7.0, 30°C Chaetomiaceae
18
-
N,N-dimethyl-1,4-phenylenediamine pH 7.0, 30°C Chaetomiaceae
26
-
2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) pH 7.0, 30°C Chaetomiaceae
34
-
2,6-dimethoxyphenol pH 7.0, 30°C Chaetomiaceae
46
-
catechol pH 7.0, 30°C Chaetomiaceae
55
-
hydroquinone pH 7.0, 30°C Chaetomiaceae
128
-
4-hydroxyindole pH 7.0, 30°C Chaetomiaceae

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
-
Chaetomiaceae

pI Value

Organism Comment pI Value Maximum pI Value
Chaetomiaceae isoelectric focusing
-
3.5